An Atopic Preventive Drink (APD) reduces Th2 cytokines in LPS-treated RAW 264.7 cells
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초록
We analyzed the effects of an Atopic Preventive Drink (APD) on the regulation of Th2 cytokines using RAW 264.7 macrophage cells. In the evaluation of nitric oxide (NO) production in cells, NO production levels were shown to be elevated only in the APD-treated group in a dose-dependent manner. In the lipopolysaccharide (LPS) with APD-treated group, NO production significantly decreased as APD concentration increased. Further, mRNA expression levels and protein concentrations of pro-inflammatory cytokines in cells were determined. Th2 stimulatory cytokine (IL-1β) and Th2 cytokine (IL-6 and IL-10) levels were significantly reduced in the LPS with APD-treated group compared to the only LPS-treated group. mRNA expression levels of inflammatory-related genes (COX-2 and iNOS) were significantly reduced in the LPS with APD-treated group compared to the only LPS-treated group. These results suggest that APD has an anti-atopic effect by reducing mRNA and proteins expressions of Th2 cytokines and inflammatory-related genes.
Abstract
We analyzed the effects of an Atopic Preventive Drink (APD) on the regulation of Th2 cytokines using RAW 264.7 macrophage cells. In the evaluation of nitric oxide (NO) production in cells, NO production levels were shown to be elevated only in the APD-treated group in a dose-dependent manner. In the lipopolysaccharide (LPS) with APD-treated group, NO production significantly decreased as APD concentration increased. Further, mRNA expression levels and protein concentrations of pro-inflammatory cytokines in cells were determined. Th2 stimulatory cytokine (IL-1β) and Th2 cytokine (IL-6 and IL-10) levels were significantly reduced in the LPS with APD-treated group compared to the only LPS-treated group. mRNA expression levels of inflammatory-related genes (COX-2 and iNOS) were significantly reduced in the LPS with APD-treated group compared to the only LPS-treated group. These results suggest that APD has an anti-atopic effect by reducing mRNA and proteins expressions of Th2 cytokines and inflammatory-related genes.
참고문헌 (20)
- Bogdan C, "Nitric oxide and the immune response", Natl Immunol, 2, pp.907~916 (2001)
- Bruch-Gerharz D, "Nitric oxide and its implications in skin homeostasis and disease - a review", Arch Dermatol Res, 290, pp.643~651 (1998)
- Grewe M, "A role for Th1 and Th2 cells in the immunopathogenesis of atopic dermatitis", Immunol Today, 19, pp.359~361 (1998)
- Helmby H, "Interleukin 1 plays a major role in the development of Th2-mediated immunity", Eur J Immunol, 34, pp.3674~3681 (2004)
- Huynh NB, "The immunological benefits of green tea(Camellia sinensis)", Int J Biol, 9, pp.10~17 (2017)
- 임남경, "Inhibitory Effect of the Leaves of Rumex crispus L. on LPS-induced Nitric Oxide Production and the Expression of iNOS and COX-2 in Macrophages", Natural Product Sciences, 20(1), pp.51~57 (2014), 한국생약학회
- Khan MS, "Preventive effect of curcumin and quercetin against nitric oxide mediated modification of goat lung cystatin", J Agric Food Chem, 57, pp.6055~6059 (2009)
- Nakae S, "IL-1 is required for allergen-specific Th2 cell activation and the development of airway hypersensitivity response", Int Immunol, 15, pp.483~490 (2003)
- Park KH, "Topical application of Rosa multiflora root extract improves atopic dermatitis-like skin lesions induced by mite antigen in NC", Nga mice, 2014, pp.37, 178~183
- Qian GM, "Anti-inflammatory and antinociceptive effects of cordymin, a peptide purified from the medicinal mushroom Cordyceps sinensis", Nat Prod Res, 26, pp.2358~2362 (2012)
- Redington AE, "Increased expression of inducible nitric oxide synthase and cyclo-oxygenase-2 in the airway epithelium of asthmatic subjects and regulation by corticosteroid treatment", Thorax, 56, pp.351~357 (2001)
- Rowe A, "Expression of inducible nitric oxide synthase in atopic dermatitis", J Investig Dermatol, 4, pp.650~ (1995)
- "2016", 6(2), 후마니타스의학회
- Singh BN, "Green tea catechin, epigallocatechin-3-gallate(EGCG) : mechanisms, perspectives and clinical applications", Biochem Pharm, 82, pp.1807~1821 (2011)
- Skehan P, "New colorimetric cytotoxicity assay for anticancer-drug screening", J Natl Cancer Inst, 82, pp.1107~1112 (1990)
- Tokura Y, "Extrinsic and intrinsic types of atopic dermatitis", J Dermatol Sci, 58, pp.1~7 (2010)
- Won TJ, "Oral administration of Lactobacillus strains from kimchi inhibits atopic dermatitis in NC⁄Nga mice", J Appl Microbiol, 110, pp.1195~1202 (2011)
- Yamamoto M, "Contribution of itch-associated scratch behavior to the development of skin lesions in dermatophagoides farinaeinduced dermatitis model in NC", Nga mice, 2009, pp.301, 739~746
- Yang ML, "Anti-inflammatory principles from Cordyceps sinensis", J Nat Pro, 74, pp.1996~2000 (2011)
- 역약곤, "Preventive Effect of the Korean Traditional Health Drink (Taemyeongcheong) on Acetaminophen-Induced Hepatic Damage in ICR Mice", Preventive Nutrition and Food Science, 20(1), pp.52~59 (2015), 한국식품영양과학회[DOI]