Anticholinesterase activity of Cinnamomum zeylanicum L. leaf extract
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Cinnamomum zeylanicum ( C. zeylanicum ) is a tropical evergreen tree of Lauraceae family. It is one of the oldest culinary spices known and used traditionally in many cultures for centuries. In addition to its culinary uses, cinnamon also possesses as a folk remedy of many health disease condition including analgesic, antiseptic, antispasmodic, aphrodisiac, astringent, carminative, haemostatic, insecticidal, and parasiticide and memory enhancing property. This study was aimed to assess the acetylcholinesterase and butyrylcholinesterase inhibitory activity of standardized methanol extract of the C. zeylanicum . Gas chromatography - mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analysis were done to identify the presence of eugenol as chemical component and support the neuroprotective activity in the extract. Anticholinesterase inhibitory activity of crude methanol extract of C. zeylanicum leaves and cinnamon oil were evaluated by 96-well microtiter plate assay and thin layer chromatography bioassay detection methods. This study revealed that cinnamon oil (IC 50 : 45.88 ± 1.94 μg/ml) has better anticholinesterase activity than methanol extract (IC 50 : 77.78 ± 0.03 μg/ml). In HPLC analysis, retention time of eugenol in cinnamon oil was found to be 15.81 min which was comparable with the retention time (15.99 min) of the reference standard, eugenol. Seven chemical compounds were identified by GC-MS analysis, in which eugenol as an important phytoconstituents. Thus the phytochemicals from C. zeylanicum methanol leaves extract could be developed as potential source of anticholinesterase activity, with particular benefit in the symptomatic treatment of Alzheimer’s disease.
Abstract
Cinnamomum zeylanicum ( C. zeylanicum ) is a tropical evergreen tree of Lauraceae family. It is one of the oldest culinary spices known and used traditionally in many cultures for centuries. In addition to its culinary uses, cinnamon also possesses as a folk remedy of many health disease condition including analgesic, antiseptic, antispasmodic, aphrodisiac, astringent, carminative, haemostatic, insecticidal, and parasiticide and memory enhancing property. This study was aimed to assess the acetylcholinesterase and butyrylcholinesterase inhibitory activity of standardized methanol extract of the C. zeylanicum . Gas chromatography - mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analysis were done to identify the presence of eugenol as chemical component and support the neuroprotective activity in the extract. Anticholinesterase inhibitory activity of crude methanol extract of C. zeylanicum leaves and cinnamon oil were evaluated by 96-well microtiter plate assay and thin layer chromatography bioassay detection methods. This study revealed that cinnamon oil (IC 50 : 45.88 ± 1.94 μg/ml) has better anticholinesterase activity than methanol extract (IC 50 : 77.78 ± 0.03 μg/ml). In HPLC analysis, retention time of eugenol in cinnamon oil was found to be 15.81 min which was comparable with the retention time (15.99 min) of the reference standard, eugenol. Seven chemical compounds were identified by GC-MS analysis, in which eugenol as an important phytoconstituents. Thus the phytochemicals from C. zeylanicum methanol leaves extract could be developed as potential source of anticholinesterase activity, with particular benefit in the symptomatic treatment of Alzheimer’s disease.
참고문헌 (27)
- Adams RP, "Identification of Essential Oils by Ion Trap Mass Spectroscopy" (2001), Academic Press
- Bhadra S, "Cholinesterase inhibition activity of Marsilea quadrifolia Linn. an edible leafy vegetable from West Bengal, India", Nat Prod Res, 26, pp.1519~1522 (2012)
- Bhadra S, "Anticholinesterase activity of standardized extract of Illicium verum Hook.f. fruits", Fitoterapia, 82, pp.342~346 (2011)
- Boga M, "Antioxidant and anticholinesterase activities of eleven edible plants", Pharm Biol, 49, pp.290~295 (2011)
- Chaudhary SK, "Angiotensin converting enzyme inhibition activity of fennel and coriander oils from India", Nat Prod Comm, 8, pp.671~672 (2013)
- Ellman GL, "A new and rapid colorimetric determination of acetylcholinesterase activity", Biochem Pharm, 7, pp.88~95 (1961)
- Gholamhoseinian A, "Screening the methanol extracts of some Iranian plants for acetylcholinesterase inhibitory activity", Res Pharm Sci, 4, pp.105~112 (2009)
- Jaganathan SK, "Antiproliferative and molecular mechanism of eugenol-induced apoptosis in cancer cells", Molecules, 17, pp.6290~6304 (2012)
- Kermasha S, "Separation and Characterization of Pepper-Contaminated cinnamon Using HPLC Analyses", J Food Sci Tech, 27, pp.578~582 (1994)
- Kovats E, "Gas chromatographic characterization of organic substances in the retention index system" (1965), Marcel Dekker
- Kumar NS, "Acetylcholinesterase inhibitory potential of a carbazole alkaloid, Mahanimbine from Murraya koenigii", Phytother Res, 24, pp.629~631 (2010)
- Kumar P, "Kinetics of enzyme inhibition by active molluscicidal agents ferulic acid, umbelliferone, eugenol and limonene in the nervous tissue of snail Lymnaea acuminate", Phytother Res, 23, pp.172~177 (2009)
- Laekeman G., "Assessment report on Cinnamomum verum J. S. Presl, cortex and corticisaetheroleum" (2011), European Medicines Agency
- Mallavarapu GR, "Investigation of the essential oil of cinnamon leaf grown at Bangalore and Hyderabad", Flavour Frag J, 10, pp.239~242 (1995)
- Mateos-Martin ML, "Profile of urinary and fecal proanthocyanidin metabolites from common cinnamon (Cinnamomum zeylanicum L.) in rats", Mol Nutr Food Res, 56, pp.671~675 (2012)
- Mukherjee PK, "Protective effect of biflavones from Araucaria bidwillii Hook in rat cerebral ischemia", reperfusion induced oxidative stress, 2007, pp.178, 221~228
- Mukherjee PK, "Evaluation of Herbal Medicinal Products", pp.3~12 (2009), Pharmaceutical Press
- Mukherjee PK, "Screening of Indian medicinal plants for acetylcholinesterase inhibitory activity", Phytother Res, 21, pp.1142~1145 (2007)
- Nyadjeu P, "Antihypertensive and vasorelaxant effects of Cinnamomum zeylanicum stem bark aqueous extract in rats", J Complement Integr Med, 8 (2011)
- O'Shea SK, "Isolation and analysis of essential oils from spices", J Chem Educ, 89, pp.665~668 (2012)
- Prasad SN, "Neuroprotective Efficacy of Eugenol and Isoeugenol in Acrylamide-Induced Neuropathy in rats: Behavioral and Biochemical evidence", Neurochem Res, 38, pp.330~345 (2013)
- Rana VS, "Chemical variability in the essential oil of Cinnamomum tamala L. Leaves from India", Nat Prod Res, 26, pp.1355~1357 (2012)
- Ranasinghe P, "Medicinal properties of ‘true’cinnamon (Cinnamomum zeylanicum): a systematic review", BMC Complement Altern Med, 13, pp.275~284 (2013)
- Ross MSF, "Analysis of cinnamon oils by high-pressure liquid chromatography", J Chromatogr, 118, pp.273~275 (1976)
- Trajano VN, "Inhibitory effect of the essential oil from Cinnamomum zeylanicum Blume leaves on some food-related bacteria", Food Sci Tech, 30, pp.771~775 (2010)
- White P, "Neocortical cholinergic neurons in elderly people", Lancet, 309, pp.668~671 (1977)
- Yang CY, "Efficacy and reliability of huperzine A in mild and moderate Alzheimer's disease", Chin J Clin Rehabil, 7, pp.4258~4259 (2003)