Effect of KH-BaRoKer-SeongJangTang based on traditional medicine theory on longitudinal bone growth
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
KH-BaRoKer-SeongJangTang (KBS) is a recently developed formulation by using traditional drugs considering traditional medical theory of Oriental books such as ShinNongBonChoGyeong and JuRye, which has been used to improve the growth of child in Korea. Although KBS is usually prescribed to many children who are in retard for their age, its pharmacological effects have not been fully understood in experimental models. The aim of this study was to evaluate the effects of KBS on bone growth. Growth plate thickness and bone parameters such as bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connection density (Conn.D), and total porosity were analyzed by means of microcomputed tomography. Serum insulin-like growth factor-I (IGF-I) levels were measured by enzyme-linked immunosorbent assay. Hepatic IGF-I mRNA expression was analyzed by real-time polymerase chain reaction. Phosphorylation of signal transducer and activator of transcription5 (STAT5) was investigated using Western blot analysis and immunohistochemistry. The thickness of growth plate was increased by KBS. BV/TV, Tb.Th, TbN, Conn.D, and total porosity were improved by KBS. Hepatic IGF-I mRNA and serum IGF-I levels were elevated by KBS. Phosphorylation of STAT5 was increased with administration of KBS. These results suggest that KBS would be helpful to children who are in retard for their age through the elevation of IGF-I.
Abstract
KH-BaRoKer-SeongJangTang (KBS) is a recently developed formulation by using traditional drugs considering traditional medical theory of Oriental books such as ShinNongBonChoGyeong and JuRye, which has been used to improve the growth of child in Korea. Although KBS is usually prescribed to many children who are in retard for their age, its pharmacological effects have not been fully understood in experimental models. The aim of this study was to evaluate the effects of KBS on bone growth. Growth plate thickness and bone parameters such as bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), connection density (Conn.D), and total porosity were analyzed by means of microcomputed tomography. Serum insulin-like growth factor-I (IGF-I) levels were measured by enzyme-linked immunosorbent assay. Hepatic IGF-I mRNA expression was analyzed by real-time polymerase chain reaction. Phosphorylation of signal transducer and activator of transcription5 (STAT5) was investigated using Western blot analysis and immunohistochemistry. The thickness of growth plate was increased by KBS. BV/TV, Tb.Th, TbN, Conn.D, and total porosity were improved by KBS. Hepatic IGF-I mRNA and serum IGF-I levels were elevated by KBS. Phosphorylation of STAT5 was increased with administration of KBS. These results suggest that KBS would be helpful to children who are in retard for their age through the elevation of IGF-I.
참고문헌 (39)
- Abad V, "The role of the resting zone in growth plate chondrogenesis", Endocrinology, 143, pp.1851~1857 (2002)
- Barnes PJ, "How corticosteroids control inflammation : Quintiles Prize Lecture 2005", Br J Pharmacol, 148, pp.245~254 (2006)
- Baron J, "Dexamethasone acts locally to inhibit longitudinal bone growth in rabbits", Am J Physiol, 263, pp.E489~E492 (1992)
- Blaser C, "Adjunctive dexamethasone affects the expression of genes related to inflammation, neurogenesis and apoptosis in infant rat pneumococcal meningitis", PLoS One, 6, pp.e17840~ (2011)
- Brooks AJ, "The growth hormone receptor : mechanism of activation and clinical implications", Nat Rev Endocrinol, 6, pp.515~525 (2010)
- Brynskov T, "Longstanding refractory pseudophakic cystoid macular edema resolved using intravitreal 0.7 mg dexamethasone implants", Clin Ophthalmol, 7, pp.1171~1174 (2013)
- Chae HJ, "Placenta hominis protects osteoporosis in ovariectomized rats", Immunopharmacol Immunotoxicol, 28, pp.165~173 (2006)
- Choi MJ, "Effect of dietary taurine and arginine supplementation on bone mineral density in growing female rats", Adv Exp Med Biol, 776, pp.335~345 (2013)
- Chrysis D, "Growth retardation induced by dexamethasone is associated with increased apoptosis of the growth plate chondrocytes", J Endocrinol, 176, pp.331~337 (2003)
- Chrysis D, "Dexamethasone induces apoptosis in proliferative chondrocytes through activation of caspases and suppression of the Akt-phosphatidylinositol3'-kinase signaling pathway", Endocrinology, 146, pp.1391~1397 (2005)
- Davey HW, "STAT5b-deficient mice are growth hormone pulse-resistant. Role of STAT5b in sex-specific liver p450 expression", J Biol Chem, 274, pp.35331~35336 (1999)
- de Vos AM, "Human growth hormone and extracellular domain of its receptor: crystal structure of the complex", Science, 255, pp.306~312 (1992)
- Fock RA, "Effects of protein-energy malnutrition on NF-kappaB signalling in murine peritoneal macrophages", Inflammation, 33, pp.101~109 (2010)
- Giuliano C, "Role of dexamethasone in the prevention of migraine recurrence in the acute care setting : a review", Postgrad Med, 124, pp.110~115 (2012)
- Han NR, "Kanamycin activates caspase-1 in NC", Nga mice, 2011, pp.20, 659~663
- Harris L, "Inflammatory bowel disease causes reversible suppression of osteoblast and chondrocyte function in mice", Am J Physiol Gastrointest Liver Physiol, 296, pp.G1020~G1029 (2009)
- Kim HJ, "Bone-protecting effect of safflower seeds in ovariectomized rats", Calcif Tissue Int, 71, pp.88~94 (2002)
- King B, "Weight control, endocrine hormones and cancer prevention", Exp Biol Med, 238, pp.502~508 (2013)
- Kugelberg M, "Glucocorticoid eye drops inhibit growth in the newborn rabbit", Acta Paediatr, 94, pp.1096~1101 (2005)
- Le Roith D, "The somatomedin hypothesis : 2001", Endocr Rev, 22, pp.53~74 (2001)
- Lee YS, "Determination of mineral content in methanolic safflower (Carthamus tinctorius L.) seed extract and its effect on osteoblast markers", Int J Mol Sci, 10, pp.292~305 (2009)
- Lui JC, "Effects of glucocorticoids on the growth plate", Endocr Dev, 20, pp.187~193 (2011)
- Moon PD, "Epigallocatechin-3-O-gallate inhibits the production of thymic stromal lymphopoietin by the blockade of caspase-1", NF-κB pathway in mast cells, 2012, pp.42, 2513~2519
- Moon PD, "Thymic stromal lymphopoietin is expressed and produced by caspase-1", NF-κB pathway in mast cells, 2011, pp.54, 239~243
- Moon PD, "Suppression of thymic stromal lymphopoietin production by rutin in mast cells", Food Chem, 133, pp.76~81 (2012)
- Ngueguim FT, "Evaluation of Cameroonian plants towards experimental bone regeneration", J Ethnopharmacol, 141, pp.331~337 (2012)
- Pennisi P, "L-arginine supplementation normalizes bone turnover and preserves bone mass in streptozotocin-induced diabetic rats", J Endocrinol Invest, 32, pp.546~551 (2009)
- Puignero V, "Effects of cyclosporine and dexamethasone on IgE antibody response in mice, and on passive cutaneous anaphylaxis in the rat", Int Arch Allergy Immunol, 108, pp.142~147 (1995)
- Rabkin R, "Growth hormone resistance in uremia, a role for impaired JAK", STAT signaling, 2005, pp.20, 313~318
- Rahman A, "Pooled analysis of two clinical trials comparing the clinical outcomes of topical ciprofloxacin", dexamethasone otic suspension and polymyxin B, hydrocortisone otic suspension for the treatment of acute otitis externa in adults and children, pp.Clin Ther, 2007[DOI]
- Reeves PG, "AIN-93 purified diets for laboratory rodents : final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet", J Nutr, 123, pp.1939~1951 (1993)
- Sharan K, "A novel quercetin analogue from a medicinal plant promotes peak bone mass achievement and bone healing after injury and exerts an anabolic effect on osteoporotic bone : the role of aryl hydrocarbon receptor as a mediator of osteogenic action", J Bone Miner Res, 26, pp.2096~2111 (2011)
- Soheilian M, "Randomized trial of intravitreal clindamycin and dexamethasone versus pyrimethamine, sulfadiazine, and prednisolone in treatment of ocular toxoplasmosis", Ophthalmology, 118, pp.134~141 (2011)
- Thompson BT, "Glucocorticoids and acute lung injury", Critl Care Med, 31, pp.S253~S257 (2003)
- Weng CY, "MAP kinase pathway is involved in IGF-1-stimulated proliferation of human retinal pigment epithelial cells(hRPE)", Curr Eye Res, 34, pp.867~876 (2009)
- Wershil BK, "Dexamethasone or cyclosporin A suppress mast cell-leukocyte cytokine cascades. Multiple mechanisms of inhibition of IgE- and mast cell-dependent cutaneous inflammation in the mouse", J Immunol, 154, pp.1391~1398 (1995)
- Woelfle J, "Mechanisms of growth hormone (GH) action. Identification of conserved Stat5 binding sites that mediate GH-induced insulin-like growth factor-I gene activation", J Biol Chem, 278, pp.51261~51266 (2003)
- Youreva V, "Insulin-like growth-factor-1-induced PKB signaling and Egr-1 expression is inhibited by curcumin in A-10 vascular smooth muscle cells", Can J Physiol Pharmacol, 91, pp.241~247 (2013)
- Zhang R, "Effects of TGF-beta1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations", J Orthop Surg Res, 1, pp.9~ (2006)