In vitro antioxidant and free radical scavenging activities of stem extract of Euphorbia trigona Miller
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Antioxidative and free radical scavenging properties of different stem extracts of Euphorbia trigona were evaluated and correlated with its total phenolic content. Aqueous, acetone and methanolic extracts of shade dried stem were obtained and were concentrated in vacuo. The antioxidant and free radical scavenging activities of stem extracts was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, deoxyribose degradation assay and Fe2+chelating assay. Total phenolic contents (TPC) were evaluated using Folin-Ciocalteu reagent. The results confirmed that the plant is a rich source of polyphenolic compounds which are invariably higher compared to other herbs. All extracts showed TPC in the range of 146.6 – 168.6 mg/g gallic acid equivalents at 300 µg/ml of extract. Among the three extracts ME showed highest scavenging activity as evidenced by maximum scavenging of DPPH (83.2%), OH• radicals (94.81%), Fe2+chelating activity (88.59%) and a high reducing power 0.623 at 300 µg/ml. Our results demonstrate that Euphorbia trigona, an unexplored xerophytic plant could be potential source of natural antioxidants and phytotherapeutic agents. The plant possess invariably high amount of polyphenolic compounds with a broad spectrum of antioxidant properties and could be further used for food, feed and pharmaceutical applications.
Abstract
Antioxidative and free radical scavenging properties of different stem extracts of Euphorbia trigona were evaluated and correlated with its total phenolic content. Aqueous, acetone and methanolic extracts of shade dried stem were obtained and were concentrated in vacuo. The antioxidant and free radical scavenging activities of stem extracts was determined by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, deoxyribose degradation assay and Fe2+chelating assay. Total phenolic contents (TPC) were evaluated using Folin-Ciocalteu reagent. The results confirmed that the plant is a rich source of polyphenolic compounds which are invariably higher compared to other herbs. All extracts showed TPC in the range of 146.6 – 168.6 mg/g gallic acid equivalents at 300 µg/ml of extract. Among the three extracts ME showed highest scavenging activity as evidenced by maximum scavenging of DPPH (83.2%), OH• radicals (94.81%), Fe2+chelating activity (88.59%) and a high reducing power 0.623 at 300 µg/ml. Our results demonstrate that Euphorbia trigona, an unexplored xerophytic plant could be potential source of natural antioxidants and phytotherapeutic agents. The plant possess invariably high amount of polyphenolic compounds with a broad spectrum of antioxidant properties and could be further used for food, feed and pharmaceutical applications.
참고문헌 (32)
- Aruoma OI, "Evidence consistent with a site-specific hydroxyl radical generation caused by iron ions bound to the deoxyribose molecule", J Inorg Biochem, 29, pp.289~299 (1987)
- Chizzola R, "Antioxidative properties of Thymus vulgaris leaves: comparison of different extracts and essential oil chemotypes", J Agr Food Chem, 56, pp.6897~6904 (2008)
- Conde-Hernandez LA, "Total phenolics and antioxidant activity of Piper auritum and Porophyllum ruderale", Food Chem, 142, pp.455~560 (2014)
- Dinis TC, "Action of phenolic derivates (acetoaminophen, salycilate and 5-aminosalycilate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers", Arch Biochem Biophys, 315, pp.161~169 (1994)
- Djeridane A, "Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds", Food Chem, 97, pp.654~660 (2006)
- Foti MC, "Electron-transfer reaction of cinnamic acids and their methyl esters with the DPPH radical in alcoholic solutions", J Org Chem, 69, pp.2309~2314 (2004)
- Hall CA, "Antioxidant methodology in vivo and in vitro concepts", pp.2~29 (1997), AOCS Press
- Halliwell B, "The deoxyribose method: A simple "test-tube" assay for determination of rate constants for reactions of hydroxyl radicals", Analyt Biochem, 165, pp.215~219 (1987)
- Halliwell B, "Free radicals in biology and medicine" (1999), Oxford University Press
- Hecker E, "New toxic, irritant and cocarcinogenic diterpene esters from Euphorbiaceae and from Thymelaeaceae", Pure App Chem, 49, pp.1423~1431 (1977)
- Honda K, "Oxidative stress and redox iron in Alzheimer's disease", Ann N Y Acad Sci, 1012, pp.179~182 (2004)
- Khare CP, "Indian Medicinal Plants: An Illustrated Dictionary" (2007), Springer Science & Business Media
- Kumar V, "Antioxidant and DNA damage protecting activities of Eulophia nuda Lindl", Free Rad Antiox, 3, pp.55~60 (2013)
- Marian V, "Free radicals and antioxidants in normal physiological functions and human disease", Int J Biochem Cell Biol, 39, pp.44~84 (2007)
- Nagmoti DM, "Antioxidant activity and free radical-scavenging potential of Pithecellobium dulce Benth seed extracts", Free Rad Antiox, 2, pp.37~43 (2012)
- Oyaizu M, "Studies on products of browning reaction: antioxidative activities of products of browning reaction prepared from glucosamine", Jpn J Nutr, 44, pp.307~315 (1986)
- Ozsoy N, "Antioxidant activity of Smilax excelsa L. leaf extracts", Food Chem, 110, pp.571~583 (2008)
- Ridnour LA, "Nitric oxide regulates angiogenesis through a functional switch involving thrombospondin-1", Proc Natl Acad Sci USA, 102, pp.13147~13152 (2005)
- Robbins RJ, "Phenolic acids in foods : An overview of analytical methodology", J Agri Food Chem, 51, pp.2866~2887 (2003)
- Salar RK, "Stress influenced increase in phenolic content and radical scavenging capacity of Rhodotorula glutinis CCY 20-2-26", 3 Biotech, 3, pp.53~60 (2013)
- Raj Kumar Salar, "Modulation of Phenolic Content and Antioxidant Activity of Maize by Solid State Fermentation with Thamnidium elegans CCF 1456", Biotechnology and Bioprocess Engineering, 17(1), pp.109~116 (2012), 한국생물공학회
- Salar RK, "Evaluation of antioxidant activity and radical scavenging property of Ficus bengalensis L. applying various spectroscopic and spin-trapping methods", J Biol Active Prod Nat, 1, pp.248~261 (2011)
- Salar RK, "Antimicrobial and free radical scavenging activity of extracts of some Indian medicinal plants", J Med Plant Res, 4, pp.2313~2320 (2010)
- Salar RK, "Free radical scavenging activity, phenolic contents and phytochemical evaluation of different extracts of stem bark of Butea monosperma (Lam.) Kuntze", Front Life Sci, 5, pp.107~116 (2011)
- Shahat AA, "Polyphenolic content and antioxidant activity of some wild Saudi Arabian asteraceae plants", Asian Pac J Trop Med, 7, pp.545~551 (2014)
- Stohs SJ, "Oxidative mechanisms in the toxicity of metal ions", Free Radic Biol Med, 18, pp.321~336 (1995)
- Surendraraj KH, "Antioxidant Potential of Water Hyacinth (Eichornia crassipes): In Vitro Antioxidant Activity and Phenolic Composition", J Aquat Food Prod Technol, 22, pp.11~26 (2013)
- Tada M, "Toxic diterpernes from Euphorbia trigona (Saiunkaku: an indoor foliage plant in Japan)", Agric Biol Chem, 53, pp.425~430 (1989)
- Valko M, "Free radicals, metals and antioxidants in oxidative stress-induced cancer", Chem Biol Interact, 160, pp.1~40 (2006)
- ". Available from :" (2008)[URL]
- Yen GC, "Antioxidant activity of various tea extracts in relation to their antimutagenicity", J Agri Food Chem, 43, pp.27~32 (1995)
- Yu L, "Antioxidant properties of hard winter wheat extracts", Food Chem, 78, pp.457~461 (2002)