Validation of chrysophanol and cordycepin as marker compounds for standardization of a new herbal mixture AST2017-01
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Rumex crispus (RC) or Cordyceps militaris (CM) has been used traditionally to treat various diseases and has been also consumed as a functional food made by humanitas medicine concept. We prepared a new herbal mixture, AST2017-01 which is mainly composed of processed (Beopje)-RC (P-RC) and -CM (P-CM). This study aims to validate marker compounds (chrysophanol and cordycepin) in P-RC and P-CM and water extracted-RC and -CM using liquid chromatography-tandem mass spectrometry. In addition, we analyzed contents of chrysophanol and cordycepin in AST2017-01. The linarites of chrysophanol and cordycepin were obtained in calibration curve with a coefficient of correlation of 0.999. The results showed that the concentrations of chrysophanol and cordycepin in P-RC and P-CM were almost 1.70 and 1.23 fold higher than that in RC and CM, respectively. Furthermore, contents of chrysophanol and cordycepin in the AST2017-01 are approximately 0.13% and 0.028%, respectively. In conclusion, these results indicate that chrysophanol and cordycepin were validated as marker compounds in the AST2017-01.
Abstract
Rumex crispus (RC) or Cordyceps militaris (CM) has been used traditionally to treat various diseases and has been also consumed as a functional food made by humanitas medicine concept. We prepared a new herbal mixture, AST2017-01 which is mainly composed of processed (Beopje)-RC (P-RC) and -CM (P-CM). This study aims to validate marker compounds (chrysophanol and cordycepin) in P-RC and P-CM and water extracted-RC and -CM using liquid chromatography-tandem mass spectrometry. In addition, we analyzed contents of chrysophanol and cordycepin in AST2017-01. The linarites of chrysophanol and cordycepin were obtained in calibration curve with a coefficient of correlation of 0.999. The results showed that the concentrations of chrysophanol and cordycepin in P-RC and P-CM were almost 1.70 and 1.23 fold higher than that in RC and CM, respectively. Furthermore, contents of chrysophanol and cordycepin in the AST2017-01 are approximately 0.13% and 0.028%, respectively. In conclusion, these results indicate that chrysophanol and cordycepin were validated as marker compounds in the AST2017-01.
참고문헌 (24)
- Eun Jeong Cho, "The cytoprotective effect of Rumex Aquaticus Herba extract against hydrogen peroxide-induced oxidative stress in AGS cells", Archives of Pharmacal Research, 39(12), pp.1739~1747 (2016), 대한약학회
- Das SK, "Medicinal uses of the mushroom Cordyceps militaris: Current state and prospects", Fitoterapia, 81, pp.961~968 (2010)
- Doui M, "Chemical differences between steamed rhubarbs with or without pre-processing with liquor", J Trad Med, 27, pp.109~114 (2010)
- Ha BG, "Antidiabetic effect of nepodin, a component of Rumex roots, and its modes of action in vitro and in vivo", Biofactors, 40, pp.436~447 (2014)
- 박돈희, "소리쟁이 추출물의 항균 및 항산화 활성에 관한 연구", Korean Chemical Engineering Research(HWAHAK KONGHAK), 44(1), pp.81~86 (2006), 한국화학공학회
- KFDA, "Guideline for standard of health functional food", pp.6~13 (2017), Korea Food & Drug Administration
- Kim HG, "Cordycepin inhibits lipopolysaccharide-induced inflammation by the suppression of NF-kappaB through Akt and p38 inhibition in RAW 264.7 macrophage cells", Eur J Pharmacol, 545, pp.192~199 (2006)
- 김진숙, "한약재 수치에 관한 연구(II) - 오수유, 황기의 수치전·후 지표물질의 함량분석 -", 생약학회지, 33(4), pp.305~307 (2002), 한국생약학회
- i mobilization and fibrinogen binding via phosphorylation of IP3R and VASP, "Archives of Pharmacal Research", 2015, 81~97, pp.대한약학회
- 이종록, "감초 지표성분 분석법 확립 및 liquiritigenin의 함량 증대를 위한 감초의 수치에 관한 연구", 대한한의학 방제학회지, 18(1), pp.145~154 (2010), 대한한의학방제학회
- 이명재, "Anti-Oxidative and Anti-Inflammatory Effects of QGC in Cultured Feline Esophageal Epithelial Cells", The Korean Journal of Physiology & Pharmacology, 17(1), pp.81~87 (2013), 대한약리학회
- Lee YM, "Studies on the processing of herbal medicines(VI), HPLC analysis of standard compounds of unprocessed and processed herbal medicines", Korean J Orien Med, 9, pp.69~72 (2003)
- Orbán-Gyapai O, "Flavonoids isolated from Rumex aquaticus exhibit neuroprotective and neurorestorative properties by enhancing neurite outgrowth and synaptophysin", CNS Neurol Disord Drug Targets, 13, pp.1458~1464 (2014)
- Qian G, "Optimization and validation of a chromatographic method for the simultaneous quantification of six bioactive compounds in Rhizoma et Radix Polygoni Cuspidati", J Pharm Pharmacol, 60, pp.107~113 (2008)
- Rao YK, "Constituents isolated from Cordyceps militaris suppress enhanced inflammatory mediator's production and human cancer cell proliferation", J Ethnopharmacol, 131, pp.363~367 (2010)
- Ruma IM, "Extract of Cordyceps militaris inhibits angiogenesis and suppresses tumor growth of human malignant melanoma cells", Int J Oncol, 45, pp.209~218 (2014)
- Ryu HW, "Suffruticosol A isolated from Paeonia lactiflora seedcases attenuates airway inflammation in mice induced by cigarette smoke and LPS exposure", J Functional Foods, 17, pp.774~784 (2015)
- Seo KH, "Mangosenone F, a furanoxanthone from Garciana mangostana, induces reactive oxygen species-mediated apoptosis in lung cancer cells and decreases xenograft tumor growth", Phytother Res, 29, pp.1753~1760 (2015)
- 신용욱, "한약의 수치에 관한 연구(제 7보) - 치자의 수치에 의한 성분변화 및 생리활성 -", 생약학회지, 34(1), pp.45~54 (2003), 한국생약학회
- Song J, "Cordyceps militaris induces tumor cell death via the caspase dependent mitochondrial pathway in HepG2 and MCF 7 cells", Mol Med Rep, 13, pp.5132~5140 (2016)
- Won SY, "Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris", J Ethnopharmacol, 96, pp.555~561 (2005)
- Yoou MS, "Cordycepin Suppresses Thymic Stromal Lymphopoietin Expression via Blocking Caspase-1 and Receptor-Interacting Protein 2 Signaling Pathways in Mast Cells", Biol Pharm Bull, 39, pp.90~96 (2016)
- Yoou MS, "Cordycepin diminishes thymic stromal lymphopoietin-induced interleukin-13 production", Eur J Pharmacol, 802, pp.1~6 (2017)
- Yue K, "The genus Cordyceps : a chemical and pharmacological review", J Pharm Pharmacol, 65, pp.474~493 (2013)
