원내 Alcohol류 소독제의 살균력 및 안정성 비교

Abstract

The purpose of this experiment is to examine antimicrobial effectiveness and stability of the 70% isopropyl alcohol (IPA) and 80% ethanol (ETOH). The experimental disinfectants are 70% IPA, 80% ETOH, 0.5% chlorhexidine gluconate, 0.5% chlorhexidine gluconate in 80% ETOH, 0.5% chlorhexidine gluconate in 70% IPA. The experimental type of culture strain is same for the antimicrobial effectiveness test in USP(= Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans, Aspergillus niger), and modified antimicrobial effectiveness test is adopted for disinfectants. The method used in this experiment is to maintain the antimicrobial effectiveness and stability for 0,7,14,21,28 days in the condition of open disinfectants bottles. Five culture strains of 0.1 ml are exposed to disinfectants of 5 ml for 15, 30 seconds, and 1, 5, 10, 15 minutes. After that, exposed 5 culture strains are inoculated with TSA (Trypticase soy agar) and SDA (Sabouraud dextroseagar). The result of antimicrobial effectiveness test shows the counts of cultured colony. 5 culture strains are sterilized within 15 seconds for 80% ETOH, 0.5% chlorhexidine gluconate in 80% ETOH. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Candida albicans are sterilized within 15 seconds for 70% IPA, 0.5% chlorhexidine gluconate in 70% IPA, but Aspergillus niger is sterilized after 5 minutes for 70% IPA, 0.5% chlorhexidine gluconate in 70% IPA. In the perspective of stability, the antimicrobial effectiveness shows similar result when adjusting the test period from 0,7,14,21 to 28 days. In conclusion, 80% ETOH is superior to 70% IPA in the bactericidal activity.