본문 바로가기
KERD
논문KCI 등재

특정 미생물에 대한 Chlorhexidine 감수성 평가

Assessment of the Susceptibility of Chlorhexidine to Specific Microorganisms

이정현|배성숙
한국치위생감염관리학회 (Korean society of Infection Control and Prevention in Dental Hygiene)|2023.09|한국임상치위생학회지 (Korean Journal of Clinical Dental Hygiene)|제11권 제3호|pp. 199-207|DOI 10.12972/kjcdh.20230019

0

조회

0

다운로드

0

인용

0

참고문헌

키워드

클로르헥시딘ChlorhexidineMicrobial sensitivity testsDisinfectant소독·살균제미생물 감수성 검사

초록

.

Abstract

Objectives: This study aimed to identify the range of susceptibility of E. coli, S. enterica, P. aeruginosa, and S. aureus to chlorhexidine. Methods: First, an antibacterial evaluation was conducted using the zone of inhibition by disc diffusion method. For the statistical analyses, Shapiro-Wilk was used to verify normality, Kruskal-Wallis' H analysis was performed for comparison between groups, and Bonferroni was carried out as a post-hoc analysis. Results: The zone of inhibition for each concentration of chlorhexidine by disc diffusion was different for each strain at 2%, 1%, and 0.1% (p<0.05). The Minimal Inhibitory Concentration (MIC) of chlorhexidine were 0.003% for E. coli, 0.005% for S. enterica, 0.009% for P. aeruginosa, and 0.006% for S. aureus; the Minimal Bactericidal Concentration (MBC) was measured as 0.1% for E. coli, 0.1% for S. enterica, 0.2% for P. aeruginosa, and 0.1% for S. aureus. Conclusions: The results of this study confirmed that the disc diffusion, MIC, MBC, and sensitivity of chlorhexidine to specific microorganisms are different depending on the microorganism type. Therefore, in the hospital environment, frequently used disinfectants should be managed more frequently, and susceptibility evaluation should be monitored more regularly.

참고문헌 (30)

  1. Schmalz G, "Biocompatibility of dental materials", pp.350~352 (2009), Springer
  2. Langslet A, "Chlorhexidine treatment of oral candidiasis in seriously diseased children", Acta Paediatr Scand, 63(6), pp.809~811 (1974)[DOI]
  3. FDA drug safety communication, "FDA requests label changes and single-use packaging for some over-the-counter topical antiseptic products to decrease risk of infection", Clin Infect Dis, 58(3), pp.1~2 (2014)
  4. 고수희, "오염된 0.5% 클로르헥시딘 수용액 제품과 관련된 Burkholderia cepacia 가성균혈증의 반복 유행", 의료관련감염관리, 22(1), pp.1~8 (2017), 대한의료관련감염관리학회
  5. Park C, "Identification of survival mechanisms for disinfectants resistance and the resistant-related factors", Korea Centers for Disease Control and Prevention, 10~ (2020)
  6. Russell AD, "Biocide use and antibiotic resistance : the relevance of laboratory findings to clinical and environmental situations", Lancet Infect Dis, 3(12), pp.794~803 (2003)[DOI]
  7. Löe H, "The effect of mouth rinses and topical application of chlorhexidine on the development of dental plaque andgingivitis in man", J Periodontal Res, 5(2), pp.79~83 (1970)[DOI]
  8. Renton Harper P, "A comparison of chlorhexidine, cetylpyridinium chloride, triclosan, and C31G mouthrinse products for plaque inhibition", J Periodontol, 67(5), pp.486~489 (1996)[DOI]
  9. Ainamo J, "Control of plaque by chemical agents", J Clin Periodontol, 4(5), pp.23~35 (1977)[DOI]
  10. 최희준, "사람치주인대섬유모세포에 의한 골결절 형성시 Chlorhexidine의 효과", Journal of Periodontal & Implant Science, 36(2), pp.375~383 (2006), 대한치주과학회
  11. "Infection Prevention & Control in Dental Settings.Glossary of terms for infection prevention and control in dental settings" (2023)[URL]
  12. Marc CS, "WHO model formulary 2008", pp.321~323 (2008), World Health Organization
  13. National Institute of Food and Drug Safety Evaluation, "Microbial Limit Test", Ministry of Food and Drug Safety, 41~46 (2019)
  14. Melvin PW, "M02 Performance standards for antimicrobial disk susceptibility tests", pp.17~40 (2018), Clinical and Laboratory Standards Institute
  15. Block C, "Association between intensity of chlorhexidine use and micro-organisms of reduced susceptibility in a hospital environment", J Hosp Infect, 51(3), pp.201~206 (2002)[DOI]
  16. 박종철, "Streptococcus mutans에 대한클로르헥시딘과 Erythrosine 광역동 치료의 상승효과", 대한소아치과학회지, 40(4), pp.241~246 (2013), 대한소아치과학회[DOI]
  17. 유민석, "국내 시판 중인 구강세정제의 구강미생물에 대한 항미생물 효과", 대한구강보건학회지, 41(3), pp.188~193 (2017), 대한예방치과·구강보건학회
  18. Poole K, "Mechanisms of bacterial biocide and antibiotic resistance", J Appl Microbiol, 92(1), pp.55S~64S (2002)[DOI]
  19. Emilson CG, "Susceptibility of various microorganisms to chlorhexidine", Scand J Dent Res, 85(4), pp.255~265 (1977)[DOI]
  20. A study for management of contamination prevention of human use antiseptic product, "Kim JH", Ministry of Food and Drug safety, 76~77 (2014)
  21. Korea Centers for Disease Control and Prevention, "Guideline for disinfection and sterilization in healthcare facilities", Korea Centers for Disease Control and Prevention, 41~42 (2014)
  22. Lamfon H, "Susceptibility of Candida albicans biofilms grown in the constant depth film fermentor to chlorhexidine, miconazole and fluconazole : a longitudinal study", J Antimicrob Chemother, 53(2), pp.383~385 (2004)[DOI]
  23. HØiby N, "The clinical impact of bacterial biofilms", Int J Oral Sci, 3(2), pp.55~65 (2011)[DOI]
  24. Bonez PC, "Chlorhexidine activity against bacterial biofilms", Am J Infect Control, 41(12), pp.119~122 (2013)[DOI]
  25. Totè K, "Inhibitory effect of biocides on the viable masses and matrices of Staphylococcus aureus and Pseudomonas aeruginosa biofilms", Appl Environ Microbiol, 76(10), pp.3135~3142 (2010)[DOI]
  26. Addy M, "Comparison of the in vivo and in vitro antibacterial properties of povidone iodine and chlorhexidine gluconate mouth rinses", J Clin Periodontol, 5(3), pp.198~205 (1978)[DOI]
  27. 임형섭, "구강소독제의 적용 시간 및 기계적 세척 여부에 따른 타액 내 세균수의 감소 효과 Salivary bacterial counts on application time of oral antiseptic agents and mechanical irrigation", Maxillofacial Plastic Reconstructive Surgery, 35(3), pp.155~160 (2013), 대한악안면성형재건외과학회
  28. Irizarry L, "Reduced susceptibility of methicillin-resistant Staphylococcus aureus to cetylpyridinium chloride and chlorhexidine", Chemotherapy, 42(4), pp.248~252 (1996)[DOI]
  29. "Infection Control. Disinfection and Sterilization. Disinfection. Regulatory Framework for Disinfectants and Sterilants" (2023)[URL]
  30. 김석호, "Salmonella Serovars from Foodborne and Waterborne Diseases in Korea, 1998-2007: Total Isolates Decreasing Versus Rare Serovars Emerging", Journal of Korean Medical Science, 25(12), pp.1693~1699 (2010), 대한의학회

관련 논문AI추천

흡인성 폐렴원인균에 대한 구강 양치액의 항균력 평가김성훈, 박혜진, 정은하, 한선영한국치위생감염관리학회 · 한국임상치위생학회지2023 · 제11권 제1호Vancomycin내성 및 감수성 Enterococcus faecalis 에 대한여러 항균제의 시험관내 항균력김신무, 신용섭, 심은숙, 이규식, 정수진, 정윤섭, 정재옥대한임상검사과학회 · 대한임상검사과학회지1997 · 제29권 제1호Povidone-Iodine과 Chlorhexidine의 적용시간에 따른 타액 내 세균수의 비교국중기, 김민정, 김서윤, 김수관, 김은석, 김재진, 김학균, 노기표, 박순낭대한구강악안면외과학회 · 대한구강악안면외과학회지2009 · 제35권 제5호Streptococcus mutans균에 대한 기능성 치약의 항균 효과김기림, 문소정, 황세미한국치위생감염관리학회 · 한국임상치위생학회지2019 · 제7권 제2호무알코올 구강양치액의 흡인성폐렴 원인균에 대한 항균 효과김성훈, 김태우, 박혜진, 한선영한국치위생감염관리학회 · 한국임상치위생학회지2023 · 제11권 제3호Cefixime의 치주병원성 세균 6종에 대한 항균 효과국중기, 김병옥, 김화숙, 박문규, 장현선대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제2호

이 간행물의 다른 논문 (6건)

치위생행위 규명을 위한 타당도 평가배수명, 곽선희, 신보미, 한수연, 임희정, 신선정pp. 133-151예방중심 계속구강관리사업에 참여한 치과위생사의 임상리더십 경험에 대한 연구: 포커스 그룹 인터뷰를 중심으로신보미, 배수명, 이효진pp. 153-16520-59세 성인의 비만에 대한 스트레스와 치주건강 관련성박은선pp. 167-176웹기반 선행학습에 따른 치위생(학)과 학생의 자기주도적 학습능력과 수용치석제거술기 수행자신감, 학습만족도에 관한 연구김해선pp. 177-185우리나라 성인의 구강검진 수검 경험에 영향을 미치는 요인: 다수준 분석을 이용하여곽선희pp. 187-198
원문 보기