Dietary Oxidized Linoleic Acids Modulate Fatty Acids in Mice
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Objective: An elevated concentration of oxidized lipids along with the abnormal accumulation of lipids has been linked to the formation of atheromatous plaque and the development of cardiovascular diseases. This study aims to investigate if consumption of different concentrations of dietary oxidized linoleic acid alters the distribution of long chain fatty acids (LCFAs) within the liver relative to plasma in mice. Methods: C57BL/6 male mice (n = 40) were divided into 4 groups: Standard chow as plain control (P group, n =10), Chow supplemented with linoleic acid 9 mg/mouse/day, linoleic control (C group, n=0), oxidized linoleic acid; 9 mg/mouse/day (A group, n=10) and oxidized linoleic acid 18 mg/mouse/day diet (B group, n=10). Liver and plasma samples were extracted, trans-esterified and subsequently analyzed using gas chromatography mass spectrometry (GC-MS) for LCFAs; palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid. Results: LCFA methyl esters were eluted and identified based on their respective physiochemical characteristics of GCMS assay with inter assay coefficient of variation percentage (CV%, 1.81-5.28%), limits of quantification and limit of detection values (2.021-11.402 mg/mL and 1.016-4.430 mg/mL) respectively. Correlation analysis of liver and plasma lipids of the mice groups yielded coefficients (r=0.96, 0.6, 0.8 and 0.33) with fatty acid percentage total of (16%, 10%, 16% and 58%) for the P, C, A and B groups respectively. Conclusion: The sustained consumption of a diet rich in oxidized linoleic acid disrupted fatty acid metabolism. The intake also resulted in elevated concentration of LCFAs that are precursors of bioactive metabolite molecule.
Abstract
Objective: An elevated concentration of oxidized lipids along with the abnormal accumulation of lipids has been linked to the formation of atheromatous plaque and the development of cardiovascular diseases. This study aims to investigate if consumption of different concentrations of dietary oxidized linoleic acid alters the distribution of long chain fatty acids (LCFAs) within the liver relative to plasma in mice. Methods: C57BL/6 male mice (n = 40) were divided into 4 groups: Standard chow as plain control (P group, n =10), Chow supplemented with linoleic acid 9 mg/mouse/day, linoleic control (C group, n=0), oxidized linoleic acid; 9 mg/mouse/day (A group, n=10) and oxidized linoleic acid 18 mg/mouse/day diet (B group, n=10). Liver and plasma samples were extracted, trans-esterified and subsequently analyzed using gas chromatography mass spectrometry (GC-MS) for LCFAs; palmitic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid. Results: LCFA methyl esters were eluted and identified based on their respective physiochemical characteristics of GCMS assay with inter assay coefficient of variation percentage (CV%, 1.81-5.28%), limits of quantification and limit of detection values (2.021-11.402 mg/mL and 1.016-4.430 mg/mL) respectively. Correlation analysis of liver and plasma lipids of the mice groups yielded coefficients (r=0.96, 0.6, 0.8 and 0.33) with fatty acid percentage total of (16%, 10%, 16% and 58%) for the P, C, A and B groups respectively. Conclusion: The sustained consumption of a diet rich in oxidized linoleic acid disrupted fatty acid metabolism. The intake also resulted in elevated concentration of LCFAs that are precursors of bioactive metabolite molecule.
참고문헌 (39)
- Whelan J, "Linoleic acid", Adv Nutr, 4, pp.311~312 (2013)
- Ahmed M, "Oxidation of lipids in foods", Sarhad J Agric, 32, pp.230~238 (2016)
- Vangaveti VN, "Hydroxyoctadecadienoic acids : Oxidised derivatives of linoleic acid and their role in inflammation associated with metabolic syndrome and cancer", Eur J Pharmacol, 785, pp.70~76 (2016)
- Marchix J, "Excessive dietary linoleic acid induces proinflammatory markers in rats", J Nutr Biochem, 26, pp.1434~1441 (2015)
- Kühn H, "Inflammation and immune regulation by 12", 15-lipoxygenases, 2006, pp.45, 334~356
- Takahashi H, "Comparative and stability analyses of 9-and 13-Oxo-octadecadienoic acids in various species of tomato", Biosci Biotechnol Biochem, 75, pp.1621~1624 (2011)
- Liermann W, "Effects of a maternal essential fatty acid and conjugated linoleic acid supplementation during late pregnancy and early lactation on hematologic and immunological traits and the oxidative and anti-oxidative status in blood plasma of neonatal calves", Animals(Basel), 11, pp.2168~ (2021)
- Houben T, "Oxidized LDL at the crossroads of immunity in non-alcoholic steatohepatitis", Biochim Biophys Acta Mol Cell Biol Lipids, 1862, pp.416~429 (2017)
- Garelnabi M, "Dietary oxidized linoleic acid lowers triglycerides via APOA5", APOClll dependent mechanisms, 2008, pp.199, 304~309
- de Carvalho CC, "The various roles of fatty acids", Molecules, 23, pp.2583~ (2018)
- Carta G, "Palmitic acid : physiological role, metabolism and nutritional implications", Front Physiol, 8, pp.902~ (2017)
- Shen MC, "Dietary stearic acid leads to a reduction of visceral adipose tissue in athymic nude mice", PLoS One, 9, pp.e104083~ (2014)
- Harvey KA, "Oleic acid inhibits stearic acid-induced inhibition of cell growth and pro-inflammatory responses in human aortic endothelial cells", J Lipid Res, 51, pp.3470~3480 (2010)
- Høstmark AT, "Percentages of oleic acid and arachidonic acid are inversely related in phospholipids of human sera", Lipids Health Dis, 12, pp.106~ (2013)
- Fiehn O, "Metabolomics by gas chromatography-mass spectrometry : combined targeted and untargeted profiling", Curr Protoc Mol Biol, 114, pp.1~ (2016)
- Barnes S, "Training in metabolomics research. I. Designing the experiment, collecting and extracting samples and generating metabolomics data", J Mass Spectrom, 51, pp.461~475 (2016)
- Patel DP, "Metabolic profiling by gas chromatography-mass spectrometry of energy metabolism in high-fat diet-fed obese mice", PLoS One, 12, pp.e0177953~ (2017)
- Sundekilde UK, "An integrated multi-omics analysis defines key pathway alterations in a diet-induced obesity mouse model", Metabolites, 10, pp.80~ (2020)
- Reis A, "A comparison of five lipid extraction solvent systems for lipidomic studies of human LDL", J Lipid Res, 54, pp.1812~1824 (2013)
- Raimondi S, "Getting lipids from glycerol : new perspectives on biotechnological exploitation of Candida freyschussii", Microb Cell Fact, 13, pp.83~ (2014)
- Michael-Jubeli R, "High-temperature gas chromatography-mass spectrometry for skin surface lipids profiling", J Lipid Res, 52, pp.143~151 (2011)
- Nicoara SC, "Development and optimization of a gas chromatography", mass spectrometry method for the analysis of thermochemolytic degradation products of phthiocerol dimycocerosate waxes found in Mycobacterium tuberculosis, 2013, pp.27, 2374~2382
- Zhang H, "GC-MS profiling of volatile components in different fermentation products of cordyceps sinensis mycelia", Molecules, 22, pp.1800~ (2017)
- Silva Figueiredo P, "Fatty acids consumption : the role metabolic aspects involved in obesity and its associated disorders", Nutrients, 9, pp.1158~ (2017)
- Alves-Bezerra M, "Triglyceride metabolism in the liver", Compr Physiol, 8, pp.1~8 (2017)
- Jump DB, "Fatty acid-regulated transcription factors in the liver", Annu Rev Nutr, 33, pp.249~269 (2013)
- Ipsen DH, "Molecular mechanisms of hepatic lipid accumulation in non-alcoholic fatty liver disease", Cell Mol Life Sci, 75, pp.3313~3327 (2018)
- Garelnabi M, "Dietary oxidized linoleic acid modulates plasma lipids beyond triglycerides metabolism", J Lipids, 2017, pp.1645828~ (2017)
- Massey KA, "Lipidomics of oxidized polyunsaturated fatty acids", Free Radic Biol Med, 59, pp.45~55 (2013)
- Parthasarathy S, "Lipid peroxidation and decomposition--conflicting roles in plaque vulnerability and stability", Biochim Biophys Acta, 1781, pp.221~231 (2008)
- Piper K, "Eicosanoids : atherosclerosis and cardiometabolic health", J Clin Transl Endocrinol, 19, pp.100216~ (2020)
- Clarke SD, "Peroxisome proliferator-activated receptors : a family of lipid-activated transcription factors", Am J Clin Nutr, 70, pp.566~571 (1999)
- Ramsden CE, "Lowering dietary linoleic acid reduces bioactive oxidized linoleic acid metabolites in humans", Prostaglandins Leukot Essent Fatty Acids, 87, pp.135~141 (2012)
- Adam AC, "High dietary arachidonic acid levels induce changes in complex lipids and immune-related eicosanoids and increase levels of oxidised metabolites in zebrafish(Danio rerio)", Br J Nutr, 117, pp.1075~1085 (2017)
- Sonnweber T, "Arachidonic acid metabolites in cardiovascular and metabolic diseases", Int J Mol Sci, 19, pp.3285~ (2018)
- Lagarde M, "Oxygenated metabolism of PUFA : analysis and biological relevance", Biochim Biophys Acta, 1851, pp.307~ (2015)
- Guyenet SJ, "Increase in adipose tissue linoleic acid of US adults in the last half century", Adv Nutr, 6, pp.660~664 (2015)
- den Hartigh LJ, "10E, 12Z-conjugated linoleic acid impairs adipocyte triglyceride storage by enhancing fatty acid oxidation, lipolysis, and mitochondrial reactive oxygen species", J Lipid Res, 54, pp.2964~2978 (2013)
- Umeno A, "Singlet oxygen induced products of linoleates, 10-and 12-(Z, E)-hydroxyoctadecadienoic acids(HODE), can be potential biomarkers for early detection of type 2 diabetes", PLoS One, 8, pp.e63542~ (2013)