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Role of Arachidonic Acid in Promoting Hair Growth

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초록

Background: Arachidonic acid (AA) is an omega-6 polyunsaturated fatty acid present in all mammalian cell membranes, and involved in the regulation of many cellular processes, including cell survival, angiogenesis, and mitogenesis. The dermal papilla, composed of specialized fibroblasts located in the bulb of the hair follicle, contributes to the control of hair growth and the hair cycle. Objective: This study investigated the effect of AA on hair growth by using in vivo and in vitro models. Methods: The effect of AA on human dermal papilla cells (hDPCs) and hair shaft elongation was evaluated by MTT assay and hair follicle organ culture, respectively. The expression of various growth and survival factors in hDPCs were investigated by western blot or immunohistochemistry. The ability of AA to induce and prolong anagen phase in C57BL/6 mice was analyzed. Results: AA was found to enhance the viability of hDPCs and promote the expression of several factors responsible for hair growth, including fibroblast growth factor-7 (FGF-7) and FGF-10. Western blotting identified the role of AA in the phosphorylation of various transcription factors (ERK, CREB, and AKT) and increased expression of Bcl-2 in hDPCs. In addition, AA significantly promoted hair shaft elongation, with increased proliferation of matrix keratinocytes, during ex vivo hair follicle culture. It was also found to promote hair growth by induction and prolongation of anagen phase in telogen-stage C57BL/6 mice. Conclusion: This study concludes that AA plays a role in promoting hair growth by increasing the expression of growth factors in hDPCs and enhancing follicle proliferation and survival.

Abstract

Background: Arachidonic acid (AA) is an omega-6 polyunsaturated fatty acid present in all mammalian cell membranes, and involved in the regulation of many cellular processes, including cell survival, angiogenesis, and mitogenesis. The dermal papilla, composed of specialized fibroblasts located in the bulb of the hair follicle, contributes to the control of hair growth and the hair cycle. Objective: This study investigated the effect of AA on hair growth by using in vivo and in vitro models. Methods: The effect of AA on human dermal papilla cells (hDPCs) and hair shaft elongation was evaluated by MTT assay and hair follicle organ culture, respectively. The expression of various growth and survival factors in hDPCs were investigated by western blot or immunohistochemistry. The ability of AA to induce and prolong anagen phase in C57BL/6 mice was analyzed. Results: AA was found to enhance the viability of hDPCs and promote the expression of several factors responsible for hair growth, including fibroblast growth factor-7 (FGF-7) and FGF-10. Western blotting identified the role of AA in the phosphorylation of various transcription factors (ERK, CREB, and AKT) and increased expression of Bcl-2 in hDPCs. In addition, AA significantly promoted hair shaft elongation, with increased proliferation of matrix keratinocytes, during ex vivo hair follicle culture. It was also found to promote hair growth by induction and prolongation of anagen phase in telogen-stage C57BL/6 mice. Conclusion: This study concludes that AA plays a role in promoting hair growth by increasing the expression of growth factors in hDPCs and enhancing follicle proliferation and survival.

참고문헌 (44)

  1. Paine E., "Arachidonic acid activates mitogen-activated protein(MAP)kinase-activated protein kinase 2 and mediates adhesion of a human breast carcinoma cell line to collagen type IV through a p38 MAP kinase-dependent pathway", J Biol Chem, 275, pp.11284~11290 (2000)
  2. Clària J, "Regulation of cell proliferation and apoptosis by bioactive lipid mediators", Recent Pat Anticancer Drug Discov, 1, pp.369~382 (2006)
  3. Cabral M., "Role of arachidonic acid metabolites on the control of non-differentiated intestinal epithelial cell growth", Int J Biochem Cell Biol, 45, pp.1620~1628 (2013)
  4. Bogatcheva NV., "Arachidonic acid cascade in endothelial pathobiology", Microvasc Res, 69, pp.107~127 (2005)
  5. Tragni E., "Arachidonic acid metabolism in HEL", 30 murine epidermal cell line, 1988, pp.280, 437~442
  6. Wen ZH., "Critical role of arachidonic acid-activated mTOR signaling in breast carcinogenesis and angiogenesis", Oncogene, 32, pp.160~170 (2013)
  7. Nishizawa Y., "Regulatory role of arachidonic acid-derived metabolites for proliferation of transformed murine Leydig cell in serum-free culture condition", Anticancer Res, 10, pp.317~322 (1990)
  8. Paus R., "In search of the"hair cycle clock" : a guided tour", Differentiation, 72, pp.489~511 (2004)
  9. Paus R., "The biology of hair follicles", N Engl J Med, 341, pp.491~497 (1999)
  10. Yang CC., "Review of hair follicle dermal cells", J Dermatol Sci, 57, pp.2~11 (2010)
  11. Skolnik P., "Human essential fatty acid deficiency : treatment by topical application of linoleic acid", Arch Dermatol, 113, pp.939~941 (1977)
  12. Janssen CI., "Long-chain polyunsaturated fatty acids(LCPUFA)from genesis to senescence : the influence of LCPUFA on neural development, aging, and neurodegeneration", Prog Lipid Res, 53, pp.1~17 (2014)
  13. Sasaki S., "Influence of prostaglandin F2alpha and its analogues on hair regrowth and follicular melanogenesis in a murine model", Exp Dermatol, 14, pp.323~328 (2005)
  14. 권오상, "Promotive Effect of Minoxidil Combined with All-trans Retinoic Acid (tretinoin) on Human Hair Growth in Vitro", Journal of Korean Medical Science, 22(2), pp.283~289 (2007), 대한의학회
  15. Messenger AG, "The culture of dermal papilla cells from human hair follicles", Br J Dermatol, 110, pp.685~689 (1984)
  16. Choi SJ., "Effects of glucocorticoid on human dermal papilla cells in vitro", J Steroid Biochem Mol Biol, 135, pp.24~29 (2013)
  17. Philpott MP., "Human hair growth in vitro", J Cell Sci, 97, pp.463~471 (1990)
  18. Yoon SY., "Induction of hair growth by insulin-like growth factor-1 in 1,763 MHz radiofrequency-irradiated hair follicle cells", PLoS One, 6, pp.e28474~ (2011)
  19. Paus R., "The induction of anagen hair growth in telogen mouse skin by cyclosporine A administration", Lab Invest, 60, pp.365~369 (1989)
  20. Jo SJ., "Valproic acid promotes human hair growth in in vitro culture model", J Dermatol Sci, 72, pp.16~24 (2013)
  21. Kim HH., "Photoprotective and anti-skin-aging effects of eicosapentaenoic acid in human skin in vivo", J Lipid Res, 47, pp.921~930 (2006)
  22. Xing-Ji Jin, "Prevention of UV-Induced Skin Damages by 11,14,17-Eicosatrienoic Acid in Hairless Mice In Vivo", Journal of Korean Medical Science, 25(6), pp.930~937 (2010), 대한의학회
  23. Yoon SY., "A role of placental growth factor in hair growth", J Dermatol Sci, 74, pp.125~134 (2014)
  24. Kwack MH., "Minoxidil activates β-catenin pathway in human dermal papilla cells : a possible explanation for its anagen prolongation effect", J Dermatol Sci, 62, pp.154~159 (2011)
  25. du Cros DL, "Fibroblast growth factor and epidermal growth factor in hair development", J Invest Dermatol, 101(1 Suppl), pp.106S~113S (1993)
  26. Marchese C., "Fibroblast growth factor 10 induces proliferation and differentiation of human primary cultured keratinocytes", J Invest Dermatol, 116, pp.623~628 (2001)
  27. Danilenko DM., "Keratinocyte growth factor is an important endogenous mediator of hair follicle growth, development, and differentiation. Normalization of the nu", nu follicular differentiation defect and amelioration of chemotherapy-induced alopecia, 1995, pp.147, 145~154
  28. Shimaoka S., "Hepatocyte growth factor", scatter factor expressed in follicular papilla cells stimulates human hair growth in vitro, 1995, pp.165, 333~338
  29. Jindo T., "Hepatocyte growth factor", scatter factor stimulates hair growth of mouse vibrissae in organ culture, 1994, pp.103, 306~309
  30. Hicklin DJ., "Role of the vascular endothelial growth factor pathway in tumor growth and angiogenesis", J Clin Oncol, 23, pp.1011~1027 (2005)
  31. Hwang KA., "Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by increasing the cysteine level via fibroblast growth factors 2 and 7 in an organ culture of mouse vibrissae hair follicles", Int J Mol Med, 29, pp.195~201 (2012)
  32. Müller-Röver S., "A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages", J Invest Dermatol, 117, pp.3~15 (2001)
  33. Botchkarev VA., "Molecular control of epithelial-mesenchymal interactions during hair follicle cycling", J Investig Dermatol Symp Proc, 8, pp.46~55 (2003)
  34. Kawano M., "Comprehensive analysis of FGF and FGFR expression in skin : FGF18 is highly expressed in hair follicles and capable of inducing anagen from telogen stage hair follicles", J Invest Dermatol, 124, pp.877~885 (2005)
  35. Konger RL., "Growth regulation of primary human keratinocytes by prostaglandin E receptor EP2 and EP3 subtypes", Biochim Biophys Acta, 1401, pp.221~234 (1998)
  36. Colombe L., "Prostanoid receptors in anagen human hair follicles", Exp Dermatol, 17, pp.63~72 (2008)
  37. Iino M., "Adenosine stimulates fibroblast growth factor-7 gene expression via adenosine A2b receptor signaling in dermal papilla cells", J Invest Dermatol, 127, pp.1318~1325 (2007)
  38. Seok Yong Ahn, "Effect of IGF-I on Hair Growth Is Related to the Anti-Apoptotic Effect of IGF-I and Up-Regulation of PDGF-A and PDGF-B", Annals of Dermatology, 24(1), pp.26~31 (2012), 대한피부과학회
  39. Brash AR., "Lipoxygenase metabolism of endogenous and exogenous arachidonate in leukocytes : GC-MS analyses of incubations in H2(18)O buffers", Prostaglandins Leukot Med, 23, pp.149~154 (1986)
  40. Sautebin L., "Preferential utilization of endogenous arachidonate by cyclo-oxygenase in incubations of human platelets", FEBS Lett, 157, pp.173~178 (1983)
  41. Geng L., "Topical or systemic 16, 16 dm prostaglandin E2 or WR-2721(WR-1065)protects mice from alopecia after fractionated irradiation", Int J Radiat Biol, 61, pp.533~537 (1992)
  42. Hanson WR., "Subcutaneous or topical administration of 16, 16 dimethyl prostaglandin E2 protects from radiation-induced alopecia in mice", Int J Radiat Oncol Biol Phys, 23, pp.333~337 (1992)
  43. Malkinson FD., "Prostaglandins protect against murine hair injury produced by ionizing radiation or doxorubicin", J Invest Dermatol, 101(1 Suppl), pp.135S~137S (1993)
  44. Roenigk HH Jr, "New topical agents for hair growth", Clin Dermatol, 6, pp.119~121 (1988)

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