Hair growth promotion by photobiomodulation therapy using different parameters: animal study
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Background: Photobiomodulation therapy (PBMT) devices for hair loss have been popular for many years. But most treatment modules are decided empirically, and no objectively accepted parameters have been proposed. We attempted to identify the optimal PBMT parameters for hair growth.Methods: A dome-shaped laser diode (LD)/light emitting diode (LED) module was applied to C57BL/6 mice. Each device had different LD modules in terms of numbers, total energy, and treatment times. Negative control and 3% minoxidil positive control were co-investigated. The 3 mW LDs with 5, 10, and 15 mm spacing were applied 3 or 5 times a week.Results: The dome-shaped LD/LED modules effectively induced earlier telogen to anagen transitions and increased hair follicle density. The effect was greater with decreasing spacing and increasing dose and treatment numbers. Immunohistochemistry analysis showed an increased β-catenin/Sonic Hedgehog expression similar to minoxidil, and an increased proliferation rate of fibroblast growth factor-7 (keratinocyte growth factor).Conclusion: Increasing LD/LED module numbers, power, and treatment numbers may increase the effect of PBMT on hair regrowth.
Abstract
Background: Photobiomodulation therapy (PBMT) devices for hair loss have been popular for many years. But most treatment modules are decided empirically, and no objectively accepted parameters have been proposed. We attempted to identify the optimal PBMT parameters for hair growth.Methods: A dome-shaped laser diode (LD)/light emitting diode (LED) module was applied to C57BL/6 mice. Each device had different LD modules in terms of numbers, total energy, and treatment times. Negative control and 3% minoxidil positive control were co-investigated. The 3 mW LDs with 5, 10, and 15 mm spacing were applied 3 or 5 times a week.Results: The dome-shaped LD/LED modules effectively induced earlier telogen to anagen transitions and increased hair follicle density. The effect was greater with decreasing spacing and increasing dose and treatment numbers. Immunohistochemistry analysis showed an increased β-catenin/Sonic Hedgehog expression similar to minoxidil, and an increased proliferation rate of fibroblast growth factor-7 (keratinocyte growth factor).Conclusion: Increasing LD/LED module numbers, power, and treatment numbers may increase the effect of PBMT on hair regrowth.
참고문헌 (27)
- Hadshiew IM, "Burden of hair loss : stress and the underestimated psychosocial impact of telogen effluvium and androgenetic alopecia", J Invest Dermatol, 123, pp.455~457 (2004)
- Anders JJ, "Low-level light", laser therapy versus photobiomodulation therapy, 2015, pp.33, 183~184
- Barolet D, "Light-emitting diodes(LEDs)in dermatology", Semin Cutan Med Surg, 27, pp.227~238 (2008)
- Adamskaya N, "Light therapy by blue LED improves wound healing in an excision model in rats", Injury, 42, pp.917~921 (2011)
- Zhang WW, "Light-emitting diode phototherapy : pain relief and underlying mechanisms", Lasers Med Sci, 37, pp.2343~2352 (2022)
- Komine N, "Activation of the extracellular signal-regulated kinase signal pathway by light emitting diode irradiation", Lasers Med Sci, 25, pp.531~537 (2010)
- Otsu W, "Blue light-emitting diode irradiation promotes transcription factor EB-mediated lysosome biogenesis and lysosomal cell death in murine photoreceptorderived cells", Biochem Biophys Res Commun, 526, pp.479~484 (2020)
- Chamorro E, "Effects of lightemitting diode radiations on human retinal pigment epithelial cells in vitro", Photochem Photobiol, 89, pp.468~473 (2013)
- Lee SY, "A prospective, randomized, placebo-controlled, double-blinded, and split-face clinical study on LED phototherapy for skin rejuvenation : clinical, profilometric, histologic, ultrastructural, and biochemical evaluations and comparison of three different treatment settings", J Photochem Photobiol B, 88, pp.51~67 (2007)
- Lee SY, "Blue and red light combination LED phototherapy for acne vulgaris in patients with skin phototype IV", Lasers Surg Med, 39, pp.180~188 (2007)
- Nguyen JK, "A dose-ranging, parallel group, split-face, singleblind phase II study of light emitting diode-red light(LED-RL)for skin scarring prevention : study protocol for a randomized controlled trial", Trials, 20, pp.432~ (2019)
- Lanzafame RJ, "The growth of human scalp hair mediated by visible red light laser and LED sources in males", Lasers Surg Med, 46, pp.373~ (2014)
- Avci P, "Low-level laser therapy for fat layer reduction : a comprehensive review", Lasers Surg Med, 45, pp.349~357 (2013)
- Jackson RF, "A double-blind, placebocontrolled randomized trial evaluating the ability of low-level laser therapy to improve the appearance of cellulite", Lasers Surg Med, 45, pp.141~147 (2013)
- Zhang Y, "cDNA microarray analysis of gene expression profiles in human fibroblast cells irradiated with red light", J Invest Dermatol, 120, pp.849~857 (2003)
- Barolet D, "Regulation of skin collagen metabolism in vitro using a pulsed 660 nm LED light source : clinical correlation with a singleblinded study", J Invest Dermatol, 129, pp.2751~2759 (2009)
- Lueangarun S, "A systematic review and metaanalysis of randomized controlled trials of United States Food and Drug Administration-approved, home-use, lowlevel light", laser therapy devices for pattern hair loss : device design and technology, 2021, pp.14, E64~E75
- Suchonwanit P, "Low-level laser therapy for the treatment of androgenetic alopecia in Thai men and women : a 24-week, randomized, double-blind, sham device-controlled trial", Lasers Med Sci, 34, pp.1107~1114 (2019)
- Kwon TR, "Potential synergistic effects of human placental extract and minoxidil on hair growth-promoting activity in C57BL", 6J mice, 2015, pp.40, 672~681
- Müller-Röver S, "A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages", J Invest Dermatol, 117, pp.3~15 (2001)
- York K, "A review of the treatment of male pattern hair loss", Expert Opin Pharmacother, 21, pp.603~612 (2020)
- Avci P, "Low-level laser(light)therapy(LLLT)for treatment of hair loss", Lasers Surg Med, 46, pp.144~151 (2014)
- Zerini I, "Cellulite treatment : a comprehensive literature review", J Cosmet Dermatol, 14, pp.224~240 (2015)
- Kim WS, "Is light-emitting diode phototherapy(LED-LLLT)really effective?", Laser Ther, 20, pp.205~215 (2011)
- Iino M, "Adenosine stimulates fibroblast growth factor-7 gene expression via adenosine A2b receptor signaling in dermal papilla cells", J Invest Dermatol, 127, pp.1825~ (2007)
- Enshell-Seijffers D, "β-catenin activity in the dermal papilla regulates morphogenesis and regeneration of hair", Dev Cell, 18, pp.633~642 (2010)
- St-Jacques B, "Sonic hedgehog signaling is essential for hair development", Curr Biol, 8, pp.1058~1068 (1998)