Can the Cytokine Analysis of the Scales on Alopecic Patch Predict the Response to Diphenylcyclopropenone Treatment in Alopecia Areata Patients?
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Background: Contact immune modulating therapy with diphenylcyclopropenone (DPCP) is a topical treatment option for extensive alopecia areata (AA). Because the response to DPCP treatment varies according to the patient, and it takes several months to evaluate the clinical effectiveness of the treatment, it is necessary to identify the factors that can predict the prognosis of the disease while treating with topical DPCP. Objective: In this study, cytokine levels in the scales of alopecic patches were investigated to identify whether they could predict response to DPCP during the early treatment period. Methods: Scale samples were taken from the alopecic patches in eight AA patients at 1 week, 2 months, and 4 months after DPCP sensitization. The patients were divided into responders and non-responders according to the clinical responses of DPCP treatment. Interferon (IFN)-gamma, interleukin (IL)-2, IL-12 and IL-10 levels of the subjects were compared in several perspectives. Results: Cytokine levels after 1 week of DPCP sensitization showed no statistically significant difference between two groups. After 4 months of treatment, IFN-gamma levels were significantly lower in responders than in non-responders. Conclusion: The results of this study show IFN-gamma levels in the scales of alopecic patches might possibly reflect the clinical response in AA patients treated with DPCP. However, initial cytokine levels could not predict the treatment response.
Abstract
Background: Contact immune modulating therapy with diphenylcyclopropenone (DPCP) is a topical treatment option for extensive alopecia areata (AA). Because the response to DPCP treatment varies according to the patient, and it takes several months to evaluate the clinical effectiveness of the treatment, it is necessary to identify the factors that can predict the prognosis of the disease while treating with topical DPCP. Objective: In this study, cytokine levels in the scales of alopecic patches were investigated to identify whether they could predict response to DPCP during the early treatment period. Methods: Scale samples were taken from the alopecic patches in eight AA patients at 1 week, 2 months, and 4 months after DPCP sensitization. The patients were divided into responders and non-responders according to the clinical responses of DPCP treatment. Interferon (IFN)-gamma, interleukin (IL)-2, IL-12 and IL-10 levels of the subjects were compared in several perspectives. Results: Cytokine levels after 1 week of DPCP sensitization showed no statistically significant difference between two groups. After 4 months of treatment, IFN-gamma levels were significantly lower in responders than in non-responders. Conclusion: The results of this study show IFN-gamma levels in the scales of alopecic patches might possibly reflect the clinical response in AA patients treated with DPCP. However, initial cytokine levels could not predict the treatment response.
참고문헌 (26)
- Gregoriou S, "Cytokines and other mediators in alopecia areata", Mediators Inflamm, 2010, pp.928030~ (2010)
- 박주희, "중증 원형탈모증에서 고용량 스테로이드 주기 요법과 Cyclosporine과 저용량 스테로이드 병합 요법의 비교 연구", 대한피부과학회지, 47(11), pp.1220~1226 (2009), 대한피부과학회
- Fiedler-Weiss VC, "Evaluation of anthralin in the treatment of alopecia areata", Arch Dermatol, 123, pp.1491~1493 (1987)[DOI]
- Mitchell AJ, "Topical photochemotherapy for alopecia areata", J Am Acad Dermatol, 12, pp.644~649 (1985)[DOI]
- Berth-Jones J, "Diphencyprone is not detectable in serum or urine following topical application", Acta Derm Venereol, 74, pp.312~313 (1994)
- Rokhsar CK, "Efficacy of topical sensitizers in the treatment of alopecia areata", J Am Acad Dermatol, 39, pp.751~761 (1998)[DOI]
- Higgins E, "Topical immunotherapy: unapproved uses, dosages, or indications", Clin Dermatol, 20, pp.515~521 (2002)[DOI]
- Ohlmeier MC, "Topical immunotherapy with diphenylcyclopropenone of patients with alopecia areata-a large retrospective study on 142 patients with a self-controlled design", J Eur Acad Dermatol Venereol, 26, pp.503~507 (2012)[DOI]
- Barahmani N, "Serum T helper 1 cytokine levels are greater in patients with alopecia areata regardless of severity or atopy", Clin Exp Dermatol, 35, pp.409~416 (2010)[DOI]
- Tembhre MK, "T-helper and regulatory T-cell cytokines in the peripheral blood of patients with active alopecia areata", Br J Dermatol, 169, pp.543~548 (2013)[DOI]
- Ito T, "The role of cytokines and chemokines in the T-cell-mediated autoimmune process in alopecia areata", Exp Dermatol, 23, pp.787~791 (2014)[DOI]
- Zoller M, "The progressive state, in contrast to the stable or regressive state of alopecia areata, is reflected in peripheral blood mononuclear cells", Exp Dermatol, 13, pp.435~444 (2004)[DOI]
- Dreher F, "Quantification of stratum corneum removal by adhesive tape stripping by total protein assay in 96-well microplates", Skin Res Technol, 11, pp.97~101 (2005)[DOI]
- Hendrix SW, "Optimization of the skin multiple analyte profile bioanalytical method for determination of skin biomarkers from D-Squame tape samples", Skin Res Technol, 13, pp.330~342 (2007)[DOI]
- De Jongh CM, "Stratum corneum cytokines and skin irritation response to sodium lauryl sulfate", Contact Dermatitis, 54, pp.325~333 (2006)[DOI]
- de Jongh CM, "Cytokines at different stratum corneum levels in normal and sodium lauryl sulphate-irritated skin", Skin Res Technol, 13, pp.390~398 (2007)[DOI]
- Voegeli R, "Increased stratum corneum serine protease activity in acute eczematous atopic skin", Br J Dermatol, 161, pp.70~77 (2009)
- Olsen EA, "Alopecia areata investigational assessment guidelines--part II. National Alopecia Areata Foundation", J Am Acad Dermatol, 51, pp.440~447 (2004)[DOI]
- Olsen EA, "SALT II: a new take on the Severity of Alopecia Tool (SALT) for determining percentage scalp hair loss", J Am Acad Dermatol, 75, pp.1268~1270 (2016)[DOI]
- Alkhalifah A, "Alopecia areata update: part II. Treatment", J Am Acad Dermatol, 62, pp.191~202 (2010)[DOI]
- Happle R, "Antigenic competition as a therapeutic concept for alopecia areata", Arch Dermatol Res, 267, pp.109~114 (1980)[DOI]
- Herbst V, "Diphenylcyclopropenone treatment of alopecia areata induces apoptosis of perifollicular lymphocytes", Eur J Dermatol, 16, pp.537~542 (2006)
- Hoffmann R, "Cytokine mRNA levels in Alopecia areata before and after treatment with the contact allergen diphenylcyclopropenone", J Invest Dermatol, 103, pp.530~533 (1994)[DOI]
- Teraki Y, "Cytokines in alopecia areata: contrasting cytokine profiles in localized form and extensive form (alopecia universalis)", Acta Derm Venereol, 76, pp.421~423 (1996)
- Freyschmidt-Paul P, "Interleukin-10-deficient mice are less susceptible to the induction of alopecia areata", J Invest Dermatol, 119, pp.980~982 (2002)[DOI]
- 박주희, "아토피피부염 환자에서 APDDR-0801 보습 제품의 치료 효능", 대한피부과학회지, 48(2), pp.93~100 (2010), 대한피부과학회