본문 바로가기
KERD
논문KCI 등재

The Increased Expression of Matrix Metalloproteinase-9 Messenger RNA in the Non-lesional Skin of Patients with Large Plaque Psoriasis Vulgaris

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류피부과학

초록

Background: A difference of the interleukin-18 (IL-18) mRNA expression among several proinflammatory genes was previously observed between large plaque (LP) psoriasis patients (more than 5 cm lesions are typical) and small plaque (SP) psoriasis patients (1∼2 cm lesions are typical). Therefore, it is necessary to test whether there is any difference in the expression of the genes that activate IL-18 or the expression of genes that are induced by IL-18. Objective: To test the differential mRNA expressions of caspase-1, STAT-6, MMP-1, MMP-2, MMP-9 and TIMP-1 according to the clinical types of psoriasis vulgaris lesions in Korean patients, we have analyzed the skin samples of psoriasis vulgaris patients. Methods: The total cellular RNA of skin samples from groups of patient with LP and SP psoriasis was analyzed by performing real-time PCR (the Taqman method) to compare the differences in the mRNA expressions. Results: The caspase-1 and STAT-6 mRNA expression levels from the SP lesional skin of the patients were increased compared with the caspase-1 and STAT-6 mRNA expression levels from SP non-lesional skin or normal skin, but these expression levels from the SP non-lesional skin were not significantly different from those of the LP non-lesional skin. Among MMP-1, MMP-2, MMP-9 and TIMP-1, the expressions of MMP-1, MMP-2 and MMP-9 mRNA were increased in the SP lesional skin compared with those of the SP non-lesional skin. The MMP-1 mRNA expressions in both the LP and SP lesional skin were increased compared with those in the normal skin (p=0.028 and p=0.007 respectively). The MMP-9 mRNA expression in the LP non-lesional skin was elevated compared with the MMP-9 mRNA expression in the SP non-lesional skin (p=0.047). The TIMP-1 mRNA expression levels from the non-lesional skin and the lesional skin of the psoriasis patients and the normal skin samples were not significantly different. Conclusion: The increased expression of MMP-9 mRNA in the LP non-lesional skin compared to that of the SP non-lesional skin in the psoriatic skin suggests that the increased MMP-9 mRNA expression is related to the large size type of lesion.

Abstract

Background: A difference of the interleukin-18 (IL-18) mRNA expression among several proinflammatory genes was previously observed between large plaque (LP) psoriasis patients (more than 5 cm lesions are typical) and small plaque (SP) psoriasis patients (1∼2 cm lesions are typical). Therefore, it is necessary to test whether there is any difference in the expression of the genes that activate IL-18 or the expression of genes that are induced by IL-18. Objective: To test the differential mRNA expressions of caspase-1, STAT-6, MMP-1, MMP-2, MMP-9 and TIMP-1 according to the clinical types of psoriasis vulgaris lesions in Korean patients, we have analyzed the skin samples of psoriasis vulgaris patients. Methods: The total cellular RNA of skin samples from groups of patient with LP and SP psoriasis was analyzed by performing real-time PCR (the Taqman method) to compare the differences in the mRNA expressions. Results: The caspase-1 and STAT-6 mRNA expression levels from the SP lesional skin of the patients were increased compared with the caspase-1 and STAT-6 mRNA expression levels from SP non-lesional skin or normal skin, but these expression levels from the SP non-lesional skin were not significantly different from those of the LP non-lesional skin. Among MMP-1, MMP-2, MMP-9 and TIMP-1, the expressions of MMP-1, MMP-2 and MMP-9 mRNA were increased in the SP lesional skin compared with those of the SP non-lesional skin. The MMP-1 mRNA expressions in both the LP and SP lesional skin were increased compared with those in the normal skin (p=0.028 and p=0.007 respectively). The MMP-9 mRNA expression in the LP non-lesional skin was elevated compared with the MMP-9 mRNA expression in the SP non-lesional skin (p=0.047). The TIMP-1 mRNA expression levels from the non-lesional skin and the lesional skin of the psoriasis patients and the normal skin samples were not significantly different. Conclusion: The increased expression of MMP-9 mRNA in the LP non-lesional skin compared to that of the SP non-lesional skin in the psoriatic skin suggests that the increased MMP-9 mRNA expression is related to the large size type of lesion.

참고문헌 (44)

  1. Lowes MA, "Pathogenesis and therapy of psoriasis", Nature, 445, pp.866~873 (2007)
  2. Austin LM, "The majority of epidermal T cells in Psoriasis vulgaris lesions can produce type 1 cytokines, interferon-gamma, interleukin-2, and tumor necrosis factor-alpha, defining TC1(cytotoxic T lymphocyte) and TH1 effector populations: a type 1 differentiation bias is also measured in circulating blood T cells in psoriatic patients", J Invest Dermatol, 113, pp.752~759 (1999)
  3. Jariwala SP, "The role of dendritic cells in the immunopathogenesis of psoriasis", Arch Dermatol Res, 299, pp.359~366 (2007)
  4. Terui T, "Role of neutrophils in induction of acute inflammation in T-cell-mediated immune dermatosis, psoriasis: a neutrophil-associated inflammationboosting loop", Exp Dermatol, 9, pp.1~10 (2000)
  5. Nickoloff BJ, "Characterization of lymphocyte-dependent angiogenesis using a SCID mouse: human skin model of psoriasis", J Investig Dermatol Symp Proc, 통권 : 5, pp.67~73 (2000)
  6. Bowcock AM, "Insights into psoriasis and other inflammatory diseases from large-scale gene expression studies", Hum Mol Genet, 10, pp.1793~1805 (2001)
  7. Rottman JB, "Potential role of the chemokine receptors CXCR3, CCR4, and the integrin alphaEbeta7 in the pathogenesis of psoriasis vulgaris", Lab Invest, 81, pp.335~347 (2001)
  8. Lew W, "Psoriasis vulgaris: cutaneous lymphoid tissue supports T-cell activation and "Type 1" inflammatory gene expression", Trends Immunol, 25, pp.295~305 (2004)
  9. Lew W, "Psoriasis genomics: analysis of proinflammatory (type 1) gene expression in large plaque(Western) and small plaque (Asian) psoriasis vulgaris", Br J Dermatol, 150, pp.668~676 (2004)
  10. Dinarello CA, "Interleukin-1 beta, interleukin-18, and the interleukin-1 beta converting enzyme", Ann N Y Acad Sci, 856, pp.1~11 (1998)
  11. Abraham M, "The role of IL-18 and IL-12 in the modulation of matrix metalloproteinases and their tissue inhibitors in monocytic cells", Int Immunol, 14, pp.1449~1457 (2002)
  12. Zhang B, "IL-18 increases invasiveness of HL-60 myeloid leukemia cells: up-regulation of matrix metalloproteinases-9 (MMP-9) expression", Leuk Res, 28, pp.91~95 (2004)
  13. Tamai K, "Interferon-gamma coordinately upregulates matrix metalloprotease (MMP)-1 and MMP-3, but not tissue inhibitor of metalloproteases(TIMP), expression in cultured keratinocytes", J Invest Dermatol, 104, pp.384~390 (1995)
  14. Hebenstreit D, "Signaling mechanisms, interaction partners, and target genes of STAT6", Cytokine Growth Factor Rev, 17, pp.173~188 (2006)
  15. Kruse N, "Quantification of cytokine mRNA expression by RT PCR in samples of previously frozen blood", J Immunol Methods, 210, pp.195~203 (1997)
  16. Heid CA, "Real time quantitative PCR", Genome Res, 6, pp.986~994 (1996)
  17. van Ruissen F, "Differential effects of detergents on keratinocyte gene expression", J Invest Dermatol, 110, pp.358~363 (1998)
  18. Mauviel A, "Cytokine regulation of metalloproteinase gene expression", J Cell Biochem, 53, pp.288~295 (1993)
  19. Saren P, "TNF-alpha and IL-1beta selectively induce expression of 92-kDa gelatinase by human macrophages", J Immunol, 157, pp.4159~4165 (1996)
  20. Lavigne MC, "Human bronchial epithelial cells express and secrete MMP-12", Biochem Biophys Res Commun, 324, pp.534~546 (2004)
  21. Wolk K, "IL-22 regulates the expression of genes responsible for antimicrobial defense, cellular differentiation, and mobility in keratinocytes: a potential role in psoriasis", Eur J Immunol, 36, pp.1309~1323 (2006)
  22. Opdenakker G, "Gelatinase B: a tuner and amplifier of immune functions", Trends Immunol, 22, pp.571~579 (2001)
  23. Fleischmajer R, "Basement membrane alterations in psoriasis are accompanied by epidermal overexpression of MMP-2 and its inhibitor TIMP-2", J Invest Dermatol, 115, pp.771~777 (2000)
  24. Suomela S, "Metalloelastase (MMP-12) and 92-kDa gelatinase (MMP-9) as well as their inhibitors, TIMP-1 and -3, are expressed in psoriatic lesions", Exp Dermatol, 10, pp.175~183 (2001)
  25. Thewes M, "Stromelysin-3 (ST-3): immunohistochemical characterization of the matrix metalloproteinase(MMP)-11 in benign and malignant skin tumours and other skin disorders", Clin Exp Dermatol, 24, pp.122~126 (1999)
  26. Suomela S, "Matrix metalloproteinase-19 is expressed by keratinocytes in psoriasis", Acta Derm Venereol, 83, pp.108~114 (2003)
  27. Nestle FO, "Plasmacytoid predendritic cells initiate psoriasis through interferon-alpha production", J Exp Med, 202, pp.135~143 (2005)
  28. Nestle FO, "Defining upstream elements of psoriasis pathogenesis: an emerging role for interferon alpha", J Invest Dermatol, 125, pp.xiv~xv (2005)
  29. Ninomiya T, "Significance of serum matrix metalloproteinases and their inhibitors on the antifibrogenetic effect of interferon- alfa in chronic hepatitis C patients", Intervirology, 44, pp.227~231 (2001)
  30. Flisiak I, "Effect of psoriasis activity on metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 in plasma and lesional scales", Acta Derm Venereol, 86, pp.17~21 (2006)
  31. Flisiak I, "Effect of psoriasis treatment on plasma concentrations of metalloproteinase-1 and tissue inhibitor of metalloproteinases-1", J Eur Acad Dermatol Venereol, 19, pp.418~421 (2005)
  32. Feliciani C, "The 72-kDa and the 92-kDa gelatinases, but not their inhibitors TIMP-1 and TIMP-2, are expressed in early psoriatic lesions", Exp Dermatol, 6, pp.321~327 (1997)
  33. Nickoloff BJ, "Cellular localization of interleukin-8 and its inducer, tumor necrosis factor-alpha in psoriasis", Am J Pathol, 138, pp.129~140 (1991)
  34. Ettehadi P, "Elevated tumour necrosis factor-alpha (TNF-alpha) biological activity in psoriatic skin lesions", Clin Exp Immunol, 96, pp.146~151 (1994)
  35. Uyemura K, "The cytokine network in lesional and lesion-free psoriatic skin is characterized by a T-helper type 1 cellmediated response", J Invest Dermatol, 101, pp.701~705 (1993)
  36. Schlaak JF, "T cells involved in psoriasis vulgaris belong to the Th1 subset", J Invest Dermatol, 102, pp.145~149 (1994)
  37. Nickoloff BJ, "Role of interferon-gamma in cutaneous trafficking of lymphocytes with emphasis on molecular and cellular adhesion events", Arch Dermatol, 124, pp.1835~1843 (1988)
  38. Szabo SK, "Identification and quantitation of interferon-gamma producing T cells in psoriatic lesions: localization to both CD4+ and CD8+ subsets", J Invest Dermatol, 111, pp.1072~1078 (1998)
  39. Giannelli G, "Altered expression of basement membrane proteins and their integrin receptors in lichen planus: possible pathogenetic role of gelatinases A and B", Lab Invest, 74, pp.1091~1104 (1996)
  40. Niimi Y, "Increased expression of matrix metalloproteinase-2, matrix metalloproteinase-9 and matrix metalloproteinase-13 in lesional skin of bullous pemphigoid", Int Arch Allergy Immunol, 139, pp.104~113 (2006)
  41. Cordiali-Fei P, "Decreased levels of metalloproteinase-9 and angiogenic factors in skin lesions of patients with psoriatic arthritis after therapy with anti-TNF-alpha", J Autoimmune Dis, 3, pp.5~ (2006)
  42. Cordiali-Fei P, "Effective therapy with anti-TNF-alpha in patients with psoriatic arthritis is associated with decreased levels of metalloproteinases and angiogenic cytokines in the sera and skin lesions", Ann N Y Acad Sci, 1110, pp.578~589 (2007)
  43. Papathoma AS, "Role of matrix metalloproteinase-9 in progression of mouse skin carcinogenesis", Mol Carcinog, 31, pp.74~82 (2001)
  44. Itoh T, "Experimental metastasis is suppressed in MMP-9-deficient mice", Clin Exp Metastasis, 17, pp.177~181 (1999)

관련 논문AI추천

Gene Expression Analysis of Inflammatory Cytokines in Korean Psoriatic Patients강래형, 박철종, 백순영, 윤영훈, 전지훈, 최용범대한피부과학회 · Annals of Dermatology2017 · 제29권 제4호Tissue and Serum Inflammatory Cytokine Levels in Korean Psoriasis Patients: A Comparison between Plaque and Guttate Psoriasis김유리, 노남경, 안규중, 윤혜정, 이양원, 최용범, 한송희대한피부과학회 · Annals of Dermatology2015 · 제27권 제6호Comparison of Th1 and Th17 Inflammatory Cytokine Profiles Between Chronic Plaque and Acute Guttate Psoriasis권미연, 박철종, 박효진, 이경호, 주현정, 최인혜대한피부과학회 · Annals of Dermatology2022 · 제34권 제3호Comparison of the Expression Profile of JunB, c-Jun, and S100A8 (Calgranulin A) in Psoriasis Vulgaris and Guttate Psoriasis박철종, 우소연, 이경호, Ji Hoon Chun, Kyung Jin Kim대한피부과학회 · Annals of Dermatology2009 · 제21권 제1호Connective Tissue Growth Factor Neutralization Aggravates the Psoriasis Skin Lesion: The Analysis of Psoriasis Model Mice and PatientsHideoki Ogawa, Hiroshi Tsushima, Iwao Sekigawa, Keigo Ikeda, Kenji Takamori, Kunihiro Hayakawa, Maki Fujishiro, Shinji Morimoto, Takuya Hirai, Tomoko Miyashita, Yasushi Suga, Yuko Yoshida대한피부과학회 · Annals of Dermatology2018 · 제30권 제1호The Expression and Role of Krüppel-Like Factor 4 in Psoriasis구상현, 김광중, 김광호, 박영한, 박은주, 조은별, 박새보미대한피부과학회 · Annals of Dermatology2014 · 제26권 제6호

이 간행물의 다른 논문 (13건)