Distribution of Malassezia Species on the Scalp in Korean Seborrheic Dermatitis Patients
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Background: Malassezia species play an important role in the pathogenesis of seborrheic dermatitis. In particular, M. restricta and M. globosa are considered to be the predominant organisms in seborrheic dermatitis of Western countries. However, species distribution of Malassezia in seborrheic dermatitis has not been clearly determined yet in Asia. Objective: To identify the distribution of Malassezia species on the scalp of seborrheic dermatitis patients in Korea using 26S rDNA PCR-RFLP analysis. Methods: A total of 40 seborrheic dermatitis patients and 100 normal healthy volunteers were included in this study. For the identification of Malassezia species, the scalp scales of the subjects were analyzed by 26S rDNA PCR-RFLP analysis. Results: The most commonly identified Malassezia species were M. restricta in the seborrheic dermatitis patients, and M. globosa in the normal controls. In the seborrheic dermatitis group, M. restricta was identified in 47.5%, M. globosa in 27.5%, M. furfur in 7.5%, and M. sympodialis in 2.5% of patients. In the healthy control group, M. globosa was identified in 32.0%,M. restricta in 25.0%, M. furfur in 8.0%, M. obtusa in 6.0%,M. slooffiae in 6.0%, and M. sympodialis in 4.0% of subjects. Conclusion: M. restricta is considered to be the most important Malassezia species in Korean seborrheic dermatitis patients. (Ann Dermatol 23(2) 156∼161, 2011)
Abstract
Background: Malassezia species play an important role in the pathogenesis of seborrheic dermatitis. In particular, M. restricta and M. globosa are considered to be the predominant organisms in seborrheic dermatitis of Western countries. However, species distribution of Malassezia in seborrheic dermatitis has not been clearly determined yet in Asia. Objective: To identify the distribution of Malassezia species on the scalp of seborrheic dermatitis patients in Korea using 26S rDNA PCR-RFLP analysis. Methods: A total of 40 seborrheic dermatitis patients and 100 normal healthy volunteers were included in this study. For the identification of Malassezia species, the scalp scales of the subjects were analyzed by 26S rDNA PCR-RFLP analysis. Results: The most commonly identified Malassezia species were M. restricta in the seborrheic dermatitis patients, and M. globosa in the normal controls. In the seborrheic dermatitis group, M. restricta was identified in 47.5%, M. globosa in 27.5%, M. furfur in 7.5%, and M. sympodialis in 2.5% of patients. In the healthy control group, M. globosa was identified in 32.0%,M. restricta in 25.0%, M. furfur in 8.0%, M. obtusa in 6.0%,M. slooffiae in 6.0%, and M. sympodialis in 4.0% of subjects. Conclusion: M. restricta is considered to be the most important Malassezia species in Korean seborrheic dermatitis patients. (Ann Dermatol 23(2) 156∼161, 2011)
참고문헌 (23)
- Janik MP, "Yeast infections: candidiasis, pityriasis versicolor. In Fitzpatrick’s dermatology in general medicine. 7th ed", pp.1822~1830 (2008), New York: McGraw-Hill
- Guého E, "The genus Malassezia with description of four new species", Antonie Van Leeuwenhoek, 69, pp.337~355 (1996)
- Sugita T, "Antifungal activities of tacrolimus and azole agents against the eleven currently accepted Malassezia species", J Clin Microbiol, 43, pp.2824~2829 (2005)
- Zisova LG, "Malassezia species and seborrheic dermatitis", Folia Med (Plovdiv), 51, pp.23~33 (2009)
- Gupta AK, "Skin diseases associated with Malassezia species", J Am Acad Dermatol, 51, pp.785~798 (2004)
- Morishita N, "Molecular analysis of Malassezia microflora from patients with pityriasis versicolor", Mycopathologia, 161, pp.61~65 (2006)
- Sugita T, "Quantitative analysis of cutaneous Malassezia in atopic dermatitis patients using real-time PCR", Microbiol Immunol, 50, pp.549~552 (2006)
- Gupta AK, "Identification and typing of Malassezia species by amplified fragment length polymorphism and sequence analyses of the internal transcribed spacer and large-subunit regions of ribosomal DNA", J Clin Microbiol, 42, pp.4253~4260 (2004)
- Gandra RF, "Genotyping by RAPD-PCR analyses of Malassezia furfur strains from pityriasis versicolor and seborrhoeic dermatitis patients", Mycopathologia, 162, pp.273~280 (2006)
- Guillot J, "A single PCR-restriction endonuclease analysis for rapid identification of Malassezia species", Lett Appl Microbiol, 31, pp.400~403 (2000)
- Makimura K, "Species identification and strain typing of Malassezia species stock strains and clinical isolates based on the DNA sequences of nuclear ribosomal internal transcribed spacer 1 regions", J Med Microbiol, 49, pp.29~35 (2000)
- Sugita T, "Sequence diversity of the intergenic spacer region of the rRNA gene of Malassezia globosa colonizing the skin of patients with atopic dermatitis and healthy individuals", J Clin Microbiol, 41, pp.3022~3027 (2003)
- 장수정, "The Investigation on the Distribution of Malassezia Yeasts on the Normal Korean Skin by 26S rDNA PCR-RFLP", Annals of Dermatology, 21(1), pp.18~26 (2009), 대한피부과학회
- 오병호, "Isolation of 19 strains of Malassezia dermatis from healthy human skin in Korea", JOURNAL OF DERMATOLOGY, 35, pp.772~777, BLACKWELL PUBLISHING
- González A, "Physiological and molecular characterization of atypical isolates of Malassezia furfur", J Clin Microbiol, 47, pp.48~53 (2009)
- Zomorodian K, "Distribution of Malassezia species in patients with psoriasis and healthy individuals in Tehran, Iran", J Cutan Pathol, 35, pp.1027~1031 (2008)
- Mirhendi H, "A simple PCR-RFLP method for identification and differentiation of 11 Malassezia species", J Microbiol Methods, 61, pp.281~284 (2005)
- Clift DC, "Seborrheic dermatitis and malignancy. An investigation of the skin flora", Acta Derm Venereol, 68, pp.48~52 (1988)
- 임세웅, "환자 피부 비늘 및 임상주에서 이중 중합효소 연쇄반응(Nested PCR)을 이용한 Malassezia 균종의 동정", 대한피부과학회지, 46(4), pp.446~452 (2008), 대한피부과학회
- Sugita T, "Quantitative analysis of the cutaneous Malassezia microbiota in 770 healthy Japanese by age and gender using a real-time PCR assay", Med Mycol, 48, pp.229~233 (2010)
- Gaitanis G, "Distribution of Malassezia species in pityriasis versicolor and seborrhoeic dermatitis in Greece. Typing of the major pityriasis versicolor isolate M. globosa", Br J Dermatol, 154, pp.854~859 (2006)
- Tajima M, "Molecular analysis of Malassezia microflora in seborrheic dermatitis patients: comparison with other diseases and healthy subjects", J Invest Dermatol, 128, pp.345~351 (2008)
- Takahata Y, "Cutaneous Malassezia flora in atopic dermatitis differs between adults and children", Br J Dermatol, 157, pp.1178~1182 (2007)