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Ethanol Extract of Peanut Sprout Exhibits a Potent Anti-Inflammatory Activity in Both an Oxazolone-Induced Contact Dermatitis Mouse Model and Compound 48/80-Treated HaCaT Cells

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주제분류피부과학

초록

Background: We developed an ethanol extract of peanutsprouts (EPS), a peanut sprout-derived natural product,which contains a high level of trans-resveratrol (176.75 μg/ml)and was shown to have potent antioxidant activity. Objective:We evaluated the potential anti-inflammatory activity of EPSby measuring its antioxidant potential in skin. Methods: Theanti-inflammatory activity of EPS was tested using two modelsof skin inflammation: oxazolone (OX)-induced contact dermatitisin mice and compound 48/80-treated HaCaT cells. Asbiomarkers of skin inflammation, cyclooxygenase-2 (COX-2)and nerve growth factor (NGF) levels were measured. Results: OX-induced contact dermatitis was suppressedmarkedly in mice that were treated with an ointment containing5% EPS as evidenced by a decrease in the extent of scalingand thickening (p<0.05) and supported by a histologicalstudy. COX-2 (messenger RNA [mRNA] and protein) andNGF (mRNA) levels, which were upregulated in the skin ofOX-treated mice, were suppressed markedly in the skin ofOX+EPS-treated mice. Consistent with this, compound48/80-induced expression of COX-2 (mRNA and protein)and NGF (mRNA) in HaCaT cells were suppressed by EPStreatment in a dose-dependent manner. As an inhibitor ofNF-κB, IκB protein levels were dose-dependently upregulatedby EPS. Fluorescence-activated cell sorting (FACS) analysisrevealed that EPS scavenged compound 48/80-inducedreactive oxygen species (ROS) in HaCaT cells. Conclusion:EPS exerts a potent anti-inflammatory activity via its antioxidantactivity in both mouse skin and compound48/80-treated HaCaT cells in vitro. Compound 48/80-treatedHaCaT cells are a useful new in vitro model of skininflammation.

Abstract

Background: We developed an ethanol extract of peanutsprouts (EPS), a peanut sprout-derived natural product,which contains a high level of trans-resveratrol (176.75 μg/ml)and was shown to have potent antioxidant activity. Objective:We evaluated the potential anti-inflammatory activity of EPSby measuring its antioxidant potential in skin. Methods: Theanti-inflammatory activity of EPS was tested using two modelsof skin inflammation: oxazolone (OX)-induced contact dermatitisin mice and compound 48/80-treated HaCaT cells. Asbiomarkers of skin inflammation, cyclooxygenase-2 (COX-2)and nerve growth factor (NGF) levels were measured. Results: OX-induced contact dermatitis was suppressedmarkedly in mice that were treated with an ointment containing5% EPS as evidenced by a decrease in the extent of scalingand thickening (p<0.05) and supported by a histologicalstudy. COX-2 (messenger RNA [mRNA] and protein) andNGF (mRNA) levels, which were upregulated in the skin ofOX-treated mice, were suppressed markedly in the skin ofOX+EPS-treated mice. Consistent with this, compound48/80-induced expression of COX-2 (mRNA and protein)and NGF (mRNA) in HaCaT cells were suppressed by EPStreatment in a dose-dependent manner. As an inhibitor ofNF-κB, IκB protein levels were dose-dependently upregulatedby EPS. Fluorescence-activated cell sorting (FACS) analysisrevealed that EPS scavenged compound 48/80-inducedreactive oxygen species (ROS) in HaCaT cells. Conclusion:EPS exerts a potent anti-inflammatory activity via its antioxidantactivity in both mouse skin and compound48/80-treated HaCaT cells in vitro. Compound 48/80-treatedHaCaT cells are a useful new in vitro model of skininflammation.

참고문헌 (34)

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