Morphological Characteristics of Residual Skin Surface Components Collected from the Surface of Facial Skin in Women of Different Age
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Background: Problems of skin aging and its prevention currently attract increasing attention with the growth of human life expectancy. The morphology of the stratum corneum (SC) is well known, but investigation of age-related changes of its structure is difficult in the absence of non-invasive sampling methods. The residual skin surface components (RSSC) that overlay the SC can be easily collected non-invasively. Objective: The aim of this study was to examine morphology of RSSC samples collected from the surface of facial skin of healthy female volunteers of different age. Methods: RSSC samples were non-invasively collected from 53 adult female volunteers (22 aged in the range 18∼25 years and 31 aged in the range 50∼73 years). The samples were analysed microscopically. Results: Distinct age-related changes were determined for lipid droplet size, corneocyte desquamation level and lipid crystal count. There was a significant (p=0.0006) decrease in lipid droplet size among older women. Similarly, significantly (p=0.0401) lower lipid crystal numbers were present in the older group. Conversely, corneocyte desquamation was significantly higher (p=0.0007) in older women. No age-related difference in microbial presence in the RSSC could be detected. Result patterns were generally similar to those previously found in male volunteers; however gender-related differences in the absolute values were revealed. Conclusion: Non-invasively collected RSSC samples allow identifying age-related changes on facial skin surface. The results of this study highlight gender-dependence of distinct elements of age-associated impairment of epidermal barrier and can be employed for developing new approaches to prevent changes associated with skin aging.
Abstract
Background: Problems of skin aging and its prevention currently attract increasing attention with the growth of human life expectancy. The morphology of the stratum corneum (SC) is well known, but investigation of age-related changes of its structure is difficult in the absence of non-invasive sampling methods. The residual skin surface components (RSSC) that overlay the SC can be easily collected non-invasively. Objective: The aim of this study was to examine morphology of RSSC samples collected from the surface of facial skin of healthy female volunteers of different age. Methods: RSSC samples were non-invasively collected from 53 adult female volunteers (22 aged in the range 18∼25 years and 31 aged in the range 50∼73 years). The samples were analysed microscopically. Results: Distinct age-related changes were determined for lipid droplet size, corneocyte desquamation level and lipid crystal count. There was a significant (p=0.0006) decrease in lipid droplet size among older women. Similarly, significantly (p=0.0401) lower lipid crystal numbers were present in the older group. Conversely, corneocyte desquamation was significantly higher (p=0.0007) in older women. No age-related difference in microbial presence in the RSSC could be detected. Result patterns were generally similar to those previously found in male volunteers; however gender-related differences in the absolute values were revealed. Conclusion: Non-invasively collected RSSC samples allow identifying age-related changes on facial skin surface. The results of this study highlight gender-dependence of distinct elements of age-associated impairment of epidermal barrier and can be employed for developing new approaches to prevent changes associated with skin aging.
참고문헌 (35)
- Christensen K, "Ageing populations: the challenges ahead", Lancet, 374, pp.1196~1208 (2009)
- Fried LP, "Investing in health to create a third demographic dividend", Gerontologist, (56 Suppl 2), pp.S167~S177 (2016)
- Blume-Peytavi U, "Age-associated skin conditions and diseases: current perspectives and future options", Gerontologist, (56 Suppl 2), pp.S230~S242 (2016)
- Farage MA, "Characteristics of the aging skin", Adv Wound Care (New Rochelle), 2, pp.5~10 (2013)
- Al-Nuaimi Y, "Skin health in older age", Maturitas, 79, pp.256~264 (2014)
- Jenkins G, "Molecular mechanisms of skin ageing", Mech Ageing Dev, 123, pp.801~810 (2002)
- Vierkötter A, "Environmental influences on skin aging and ethnic-specific manifestations", Dermatoendocrinol, 4, pp.227~231 (2012)
- Kuwazuru O, "Skin wrinkling morphology changes suddenly in the early 30s", Skin Res Technol, 18, pp.495~503 (2012)
- Stevenson S, "Effect of estrogens on skin aging and the potential role of SERMs", Clin Interv Aging, 2, pp.283~297 (2007)
- Emmerson E, "The role of estrogen deficiency in skin ageing and wound healing", Biogerontology, 13, pp.3~20 (2012)
- Anunciato TP, "Carotenoids and polyphenols in nutricosmetics, nutraceuticals, and cosmeceuticals", J Cosmet Dermatol, 11, pp.51~54 (2012)
- Jenkins G, "Wrinkle reduction in post-menopausal women consuming a novel oral supplement: a double-blind placebo-controlled randomized study", Int J Cosmet Sci, 36, pp.22~31 (2014)
- Proksch E, "The skin: an indispensable barrier", Experim Dermatol, 17, pp.1063~1072 (2008)
- Menon GK, "The structure and function of the stratum corneum", Int J Pharm, 435, pp.3~9 (2012)
- Shetage SS, "Effect of ethnicity, gender and age on the amount and composition of residual skin surface components derived from sebum, sweat and epidermal lipids", Skin Res Technol, 20, pp.97~107 (2014)
- Schmid-Wendtner MH, "The pH of the skin surface and its impact on the barrier function", Skin Pharmacol Physiol, 19, pp.296~302 (2006)
- Ali SM, "Skin pH: from basic science to basic skin care", Acta Derm Venereol, 93, pp.261~267 (2013)
- Chalyk NE, "Agerelated differences in morphological characteristics of residual skin surface components collected from the surface of facial skin of healthy male volunteers", Skin Res Technol, 23, pp.212~220 (2017)
- González-Serva A, "Demonstration of polarizable crystals in fresh comedonal extracts: sebum crystallizes", Acta Derm Venereol, 84, pp.418~421 (2004)
- Tóth BI, ""Sebocytes'makeup": novel mechanisms and concepts in the physiology of the human sebaceous glands", Pflugers Arch, 461, pp.593~606 (2011)
- Thiele JJ, "The antioxidant network of the stratum corneum", Curr Probl Dermatol, 29, pp.26~42 (2001)
- Passi S, "Lipophilic antioxidants in human sebum and aging", Free Radic Res, 36, pp.471~477 (2002)
- Luebberding S, "Age-related changes in skin barrier function - quantitative evaluation of 150 female subjects", Int J Cosmet Sci, 35, pp.183~190 (2013)
- Boireau-Adamezyk E, "Age-dependent changes in stratum corneum barrier function", Skin Res Technol, 20, pp.409~415 (2014)
- Shah MG, "Estrogen and skin. An overview", Am J Clin Dermatol, 2, pp.143~150 (2001)
- Piérard GE, "Revisiting the cutaneous impact of oral hormone replacement therapy", Biomed Res Int, 2013, pp.971760~ (2013)
- Slominski A, "Steroidogenesis in the skin: implications for local immune functions", J Steroid Biochem Mol Biol, 137, pp.107~123 (2013)
- Chang KC, "Estrogen receptor beta is a novel therapeutic target for photoaging", Mol Pharmacol, 77, pp.744~750 (2010)
- Zouboulis CC, "Sexual hormones in human skin", Horm Metab Res, 39, pp.85~95 (2007)
- Seo YJ, "Regional difference in sebum production by androgen susceptibility in human facial skin", Exp Dermatol, 23, pp.70~72 (2014)
- Chu M, "Documentation of normal stratum corneum scaling in an average population: features of differences among age, ethnicity and body site", Br J Dermatol, 164, pp.497~507 (2011)
- Pomari E, "Intracrine sex steroid synthesis and signaling in human epidermal keratinocytes and dermal fibroblasts", FASEB J, 29, pp.508~524 (2015)
- Inoue T, "The role of estrogen-metabolizing enzymes and estrogen receptors in human epidermis", Mol Cell Endocrinol, 344, pp.35~40 (2011)
- Zapata HJ, "The microbiota and microbiome in aging: potential implications in health and age-related diseases", J Am Geriatr Soc, 63, pp.776~781 (2015)
- Luebberding S, "Skin physiology in men and women: in vivo evaluation of 300 people including TEWL, SC hydration, sebum content and skin surface pH", Int J Cosmet Sci, 35, pp.477~483 (2013)