Effects of Particulate Matter in a Mouse Model of Oxazolone-Induced Atopic Dermatitis
3
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Background: Recent epidemiological studies have demonstrated that air pollution is associated with the inflammatory response and may aggravate inflammatory skin diseases such as atopic dermatitis (AD). However, it is unclear whether particulate matter (PM) aggravates AD symptoms. Objective: The aim of this study was to investigate whether PM exposure affects the skin barrier dysfunction and aggravates AD symptoms using human keratinocytes (HaCaT) cells and a mouse model of oxazolone-induced AD-like skin. Methods: Standard reference material (SRM) 1649b, which mainly comprises polycyclic aromatic hydrocarbons, was used as the reference PM. HaCaT cells and mouse model of oxazolone-induced AD-like skin were treated with PM. The mRNA or protein expression levels of stratum corneum (SC) and tight junction (TJ) proteins, inflammatory cytokines, as well as clinical and histological changes of the AD-like skin of mouse model were evaluated. The expression of genes and proteins was analyzed by real-time polymerase chain reaction and Western blotting. Levels of inflammatory cytokines were measured by enzyme-linked immunosorbent assay. Results: The results revealed that PM downregulates the expression levels of several SC and TJ-related proteins in the mouse model with AD-like skin. Clinically, epidermal and dermal thickness was significantly increased and dermal inflammation was prominent in PM treated AD-like skin. Conclusion: In conclusion, we found that PM aggravates skin barrier dysfunction, clinically augmenting epidermal and dermal thickening with dermal inflammation in AD-like skin. These results suggest that PM may trigger the exacerbation of AD symptoms via skin barrier dysfunction-related mechanisms.
Abstract
Background: Recent epidemiological studies have demonstrated that air pollution is associated with the inflammatory response and may aggravate inflammatory skin diseases such as atopic dermatitis (AD). However, it is unclear whether particulate matter (PM) aggravates AD symptoms. Objective: The aim of this study was to investigate whether PM exposure affects the skin barrier dysfunction and aggravates AD symptoms using human keratinocytes (HaCaT) cells and a mouse model of oxazolone-induced AD-like skin. Methods: Standard reference material (SRM) 1649b, which mainly comprises polycyclic aromatic hydrocarbons, was used as the reference PM. HaCaT cells and mouse model of oxazolone-induced AD-like skin were treated with PM. The mRNA or protein expression levels of stratum corneum (SC) and tight junction (TJ) proteins, inflammatory cytokines, as well as clinical and histological changes of the AD-like skin of mouse model were evaluated. The expression of genes and proteins was analyzed by real-time polymerase chain reaction and Western blotting. Levels of inflammatory cytokines were measured by enzyme-linked immunosorbent assay. Results: The results revealed that PM downregulates the expression levels of several SC and TJ-related proteins in the mouse model with AD-like skin. Clinically, epidermal and dermal thickness was significantly increased and dermal inflammation was prominent in PM treated AD-like skin. Conclusion: In conclusion, we found that PM aggravates skin barrier dysfunction, clinically augmenting epidermal and dermal thickening with dermal inflammation in AD-like skin. These results suggest that PM may trigger the exacerbation of AD symptoms via skin barrier dysfunction-related mechanisms.
참고문헌 (47)
- Kim KE, "Air pollution and skin diseases : adverse effects of airborne particulate matter on various skin diseases", Life Sci, 152, pp.126~134 (2016)
- Lee CW, "Eupafolin ameliorates COX-2 expression and PGE2production in particulate pollutants-exposed human keratinocytes through ROS", MAPKs pathways, 2016, pp.189, 300~309
- Pan TL, "The impact of urban particulate pollution on skin barrier function and the subsequent drug absorption", J Dermatol Sci, 78, pp.51~60 (2015)
- Kappos AD, "Health effects of particles in ambient air", Int J Hyg Environ Health, 207, pp.399~407 (2004)
- Anderson JO, "Clearing the air : a review of the effects of particulate matter air pollution on human health", J Med Toxicol, 8, pp.166~175 (2012)
- Mancebo SE, "Recognizing the impact of ambient air pollution on skin health", J Eur Acad Dermatol Venereol, 29, pp.2326~2332 (2015)
- Martinelli N, "Air particulate matter and cardiovascular disease : a narrative review", Eur J Intern Med, 24, pp.295~302 (2013)
- Brook RD, "Particulate matter air pollution and cardiovascular disease : an update to the scientific statement from the American Heart Association", Circulation, 121, pp.2331~2378 (2010)
- Krutmann J, "Pollution and skin : from epidemiological and mechanistic studies to clinical implications", J Dermatol Sci, 76, pp.163~168 (2014)
- Hänel KH, "Cytokines and the skin barrier", Int J Mol Sci, 14, pp.6720~6745 (2013)
- Rawlings AV, "Moisturization and skin barrier function", Dermatol Ther, 17(Suppl 1), pp.43~48 (2004)
- Wickett RR, "Structure and function of the epidermal barrier", Am J Infect Control, 34(Suppl 2), pp.S98~S110 (2006)
- Egawa G, "Barrier dysfunction in the skin allergy", Allergol Int, 67, pp.3~11 (2018)
- Li Q, "Effects of ambient fine particles PM2.5 on human HaCaT cells", Int J Environ Res Public Health, 14, pp.72~ (2017)
- Zhao R, "Nasal epithelial barrier disruption by particulate matter ≤2.5 μm via tight junction protein degradation", J Appl Toxicol, 38, pp.678~687 (2018)
- Lee CW, "Urban particulate matter down-regulates filaggrin via COX2expression", PGE2 production leading to skin barrier dysfunction, 2016, pp.6, 27995~
- Jin SP, "Urban particulate matter in air pollution penetrates into the barrierdisrupted skin and produces ROS-dependent cutaneous inflammatory response in vivo", J Dermatol Sci, 91, pp.175~183 (2018)
- Lademann J, "Penetration of microparticles into human skin", Hautarzt, 55, pp.1117~1119 (2004)
- Ahn K, "The role of air pollutants in atopic dermatitis", J Allergy Clin Immunol, 134, pp.993~999 (2014)
- Cork MJ, "Epidermal barrier dysfunction in atopic dermatitis", J Invest Dermatol, 129, pp.1892~1908 (2009)
- Kim JY, "Time-dependent progression from the acute to chronic phases in atopic dermatitis induced by epicutaneous allergen stimulation in NC", Nga mice, 2014, pp.23, 53~57
- Jung Y, "Eupatilin, an activator of PPARα, inhibits the development of oxazolone-induced atopic dermatitis symptoms in Balb", c mice, 2018, pp.496, 508~514
- Morgenstern V, "Atopic diseases, allergic sensitization, and exposure to traffic-related air pollution in children", Am J Respir Crit Care Med, 177, pp.1331~1337 (2008)
- Kim J, "Symptoms of atopic dermatitis are influenced by outdoor air pollution", J Allergy Clin Immunol, 132, pp.495~498.e1 (2013)
- Huss-Marp J, "Influence of short-term exposure to airborne Der p 1 and volatile organic compounds on skin barrier function and dermal blood flow in patients with atopic eczema and healthy individuals", Clin Exp Allergy, 36, pp.338~345 (2006)
- Pénard-Morand C, "Long-term exposure to closeproximity air pollution and asthma and allergies in urban children", Eur Respir J, 36, pp.33~40 (2010)
- Suárez-Varela MM, "The impact of atmospheric pollutants on the prevalence of atopic eczema in 6-7-year-old schoolchildren in Spain;ISAAC Phase III", Iran J Allergy Asthma Immunol, 12, pp.220~227 (2013)
- 김혜원, "Improvement of Atopic Dermatitis Severity after Reducing Indoor Air Pollutants", Annals of Dermatology, 25(3), pp.292~297 (2013), 대한피부과학회
- Kim M, "Particulate matter induces pro-inflammatory cytokines via phosphorylation of p38 MAPK possibly leading to dermal inflammaging", Exp Dermatol, 28, pp.809~815 (2019)
- Kim JH, "Afzelin suppresses proinflammatory responses in particulate matterexposed human keratinocytes", Int J Mol Med, 43, pp.2516~2522 (2019)
- Jin H, "Animal models of atopic dermatitis", J Invest Dermatol, 129, pp.31~40 (2009)
- Man MQ, "Characterization of a hapten-induced, murine model with multiple features of atopic dermatitis: structural, immunologic, and biochemical changes following single versus multiple oxazolone challenges", J Invest Dermatol, 128, pp.79~86 (2008)
- Kim SY, "Effects of inhaled particulate matter on the central nervous system in mice", Neurotoxicology, 67, pp.169~177 (2018)
- 김병곤, "Long-Term Effects of Diesel Exhaust Particles on Airway Inflammation and Remodeling in a Mouse Model", Allergy, Asthma & Immunology Research, 8(3), pp.246~256 (2016), 대한천식알레르기학회
- Hanifin JM, "Evolving concepts of pathogenesis in atopic dermatitis and other eczemas", J Invest Dermatol, 129, pp.320~322 (2009)
- Kim BE, "Loricrin and involucrin expression is down-regulated by Th2 cytokines through STAT-6", Clin Immunol, 126, pp.332~337 (2008)
- Gruber R, "Filaggrin genotype in ichthyosis vulgaris predicts abnormalities in epidermal structure and function", Am J Pathol, 178, pp.2252~2263 (2011)
- De Benedetto A, "Tight junction defects in patients with atopic dermatitis", J Allergy Clin Immunol, 127, pp.773~786 (2011)
- Lehmann AD, "Diesel exhaust particles modulate the tight junction protein occludin in lung cells in vitro", Part Fibre Toxicol, 6, pp.26~ (2009)
- Stojadinovic O, "Deregulation of keratinocyte differentiation and activation : a hallmark of venous ulcers", J Cell Mol Med, 12, pp.2675~2690 (2008)
- Shreffler WG, "The major glycoprotein allergen from Arachis hypogaea, Ara h 1, is a ligand of dendritic cell-specific ICAM-grabbing nonintegrin and acts as a Th2adjuvant in vitro", J Immunol, 177, pp.3677~3685 (2006)
- Traidl-Hoffmann C, "Pollen-associated phytoprostanes inhibit dendritic cell interleukin-12 production and augment T helper type 2 cell polarization", J Exp Med, 201, pp.627~636 (2005)
- Di Nardo A, "Ceramide and cholesterol composition of the skin of patients with atopic dermatitis", Acta Derm Venereol, 78, pp.27~30 (1998)
- Lefebvre MA, "Evaluation of the impact of urban pollution on the quality of skin : a multicentre study in Mexico", Int J Cosmet Sci, 37, pp.329~338 (2015)
- Lefebvre MA, "Consequences of urban pollution upon skin status. A controlled study in Shanghai area", Int J Cosmet Sci, 38, pp.217~223 (2016)
- Liao Z, "The impact of particulate matter (PM2.5) on skin barrier revealed by transcriptome analysis:focusing on cholesterol metabolism", Toxicol Rep, 7, pp.1~9 (2019)
- Proksch E, "Skin barrier function, epidermal proliferation and differentiation in eczema", J Dermatol Sci, 43, pp.159~169 (2006)