Petunia에 도입된 bar Gene의 세대진전에 따른발현 양상
Expression in Successive Generations of bar Gene Introduced in Petunia
0
조회
0
다운로드
0
인용
0
참고문헌
초록
Agrobacterium을 이용하여 도입된 유전자의 세대진전, 교잡 등에 따른 유전적 안정성을 확인코자, 형질 전환으로부터 얻어진 bar 유전자가 도입된 형질전환 식물체들을 상호 교배, 여교배, T4세대 까지의 자식의 반복 등에 의해 유전적 안정성을 검토 하였다. 조합이나 계통에 따라서는 일부 멘델식 분리를 따르지 않고 제초제 Basta에 대한 저항성이 사라지거나 저항성개체보다 감수성개체가 기대치보다 많은 등의 경우가 있었으나, 대부분 멘델식 분리를따르고 있어 세대진전, 교배 등에 의해서도 유전적 안정성이 높게 유지됨을 확인할 수 있었다.
Abstract
This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacterium-mediated transformation, in successive generation, or after crossing or back-crossing. Some of different 25 transgenic plants were used in crossing and back-crossing to wild type, or repeated-selfing to T4 generation. On the processing of experiment, it was found that some lines lost their resistant ability to herbicide basta, or showed non-Mendelian segregation mode: produced much more susceptible segregants than resistant plants. Even though there are exceptional cases, which was off from expected, the genetic stability of bar gene introduced could be confirmed strongly, because in almost case, the segregation of resistant and susceptible plants to basta was done under Mendelian-law according to single gene dominant model.서 론
참고문헌 (14)
- Christou P, "Inheritance and expression of foreign genes in transgenic soybean plants", 86, pp.1991~1995 (1989)
- Cooley J, "Molecular and genetic characteriztion of elite transgenic rice plants produced by electric-discharge particle acceleration", 90, pp.97~104 (1995)
- Gahakwa D,, "Transgenic rice as a system to study the stability of transgene expression: multiple heterologous transgenes show similar behaviour in diverse genetic backgrounds", 101, pp.388~399 (2000)
- Ha YM, "Expression and inheritance of bar gene in petunia hybrida transformed with Agrobacterium", 30, pp.143~149 (2003)
- James C, "Global review of commercialized transgenic crop", 24 (2003)
- Kuai B, "Regeneration of fertile transgenic tall fescue plants with a stable highly expressed foreign gene", 58, pp.149~154 (1999)
- Lee SW, "Current research on the development of genetically modified plants in Korea", 30, pp.1~6 (2003)
- Rathore KS, "Use of bar as a selectable marker gene and for the production of herbicide-resistant rice plants from protoplasts", 21, pp.871~884 (1993)
- McCabe MS,, "Increased stable inheritance of herbicide resistance in transgenic lettuce carrying a petE promoter-bar gene compared with a CaMV 35S-bar gene", 99, pp.587~592 (1999)
- Ulian EC, "DNA methylation and expression of NPTII in transgenic petunias and progeny", 92, pp.976~981 (1996)
- Ulian EC, "Expression and inheritance pattern of two foreign genes in petunia", 88, pp.433~440 (1994)
- Umbeck P, "Inheritance and expression of genes for kanamycin and chloramphenicol resistance in transgenic cotton plants", 29, pp.196~201 (1989)
- Won YJ, "Establishment of a new breeding scheme for rapid release of variety using bar gene transformed rice", 31, pp.7~11 (2004)
- Zhang S,, "Variation in the inheritance of expression among subclones for unselected (uidA) and selected (bar) transgenes in maize (Zea mays L", 92, pp.752~761 (1996)