퉁퉁마디로부터 색소체 외막 단백질 유전자의 분리 및 발현분석
Molecular Cloning and Characterization of Outer Envelope Membrane Protein from Salicornia herbacea
1
조회
0
다운로드
5
인용
0
참고문헌
초록
Abstract
Complementary DNA encoding chloroplast outer envelope membrane protein (OEP) from the halophyte Salicornia herbacea has been cloned and sequenced. The full length cDNA is 596 bp and encodes a polypeptide of 91 amino acid residues with a molecular mass of 8.9 kDa. The expression level of ShOEP increased by salt, drought and ABA treatments. ShOEP expression was largely induced in roots and shoots by high salts. The biological function of ShOEP was examined by yeast complementation. ShOEP can suppress Na+ sensitivity of yeast mutant (cnb ) in the presence of salt. These results suggest that ShOEP is a salt inducible gene and may have functions in the regulation of plant salt stress.
참고문헌 (13)
- Brent R, "Current protocols in molecular Biology" (1989), Greene Publishing Associates and Wiley-Interscience
- Binzel ML, "Intracellular compartmentation of ions in salt adapted tobacco cells", 86, pp.607~614 (1988)
- Church GM, "Genomic sequencing", 81, pp.1991~5 (1984)
- Delauney AJ, "Proline biosynthesis and osmoregulation in plants", 4, pp.215~223 (1993)
- Ferro M,, "Integral membrane proteins of the chloroplast envelope: Identification and subcellular localization of new transporters", 99, pp.11487~11492 (2002)
- Fischer K, "The 24 kDa outer envelope membrane protein from spinach chloroplasts in vivo expression and import pathway of a protein with unusual properties", pp.167~177 (1994)
- Fl gge UI, "Transport in and out of plastids: does the outer envelope membrane control the flow?", 5(4), pp.135~137 (2000)
- Garbarino J, "Rapid induction of Na+", H+ exchange activity in barley root tonoplast, 1989, pp.89, 1~4
- Hanson AD, "Osmoprotective compounds in the Plumbaginaceae a natural experiment in metabolic engineering of stress tolerance", pp.306~310 (1994)
- Linke D,, "In vitro reconstitution and biophysical characterization of OEP16, an outer envelope pore protein of pea chloroplasts", 39, pp.11050~11056 (2000)
- Vartanian N, "Characterization of a novel protein induced by progressive or rapid drought and salinity in Brassica napus leaves", per(llet jc), pp.1486~1493 (1992)
- Sambrook J, "Molecular cloning: A laboratory manual, Ed 3" (2000)
- Shure M, "Molecular identification and isolation of the waxy locus in maize", 35, pp.225~33 (1983)