배추 원형질체 배양에 미치는 다양한 처리의 효과와 원형질체 유래 캘러스로부터 신초 재분화
Effects of a variety of treatments affecting Chinese cabbage protoplast culture, and plant regeneration from protoplast-derived callus
1
조회
0
다운로드
2
인용
0
참고문헌
초록
Here we describe a procedure for Chinese cabbage protoplast culture and effects of various treatments. Chinese cabbage protoplasts were isolated from different parts of young seedlings as using an enzyme mixture, of which yield was maximized in seven hours around after digestion. The highest rate of initial cell division followed by micro-callus formation was obtained in the medium with 1.0 mg/L 2,4-D, 0.5 mg/L NAA, and 1.0 mg/L BA when the cotyledon-derived protoplasts were cultured. Initiation of cell division and micro-callus proliferation significantly depended upon Chinese cabbage genotype under a same culture circumstances. The micro-calli developed from cotyledon tissue of Norang-Bom cultivar successfully grew toward callus colonies on the solidified medium with 0.2 mg/L zeatin and 0.1 mM spermidine. The callus colonies generated de novo shoots at the maximum frequency of 4.3% on the medium with 5.0 mg/L BA and 1.0 mg/L NAA. Our results might be helpful for further studies to enhance the regeneration efficiency in Chinese cabbage protoplast culture.
Abstract
Here we describe a procedure for Chinese cabbage protoplast culture and effects of various treatments. Chinese cabbage protoplasts were isolated from different parts of young seedlings as using an enzyme mixture, of which yield was maximized in seven hours around after digestion. The highest rate of initial cell division followed by micro-callus formation was obtained in the medium with 1.0 mg/L 2,4-D, 0.5 mg/L NAA, and 1.0 mg/L BA when the cotyledon-derived protoplasts were cultured. Initiation of cell division and micro-callus proliferation significantly depended upon Chinese cabbage genotype under a same culture circumstances. The micro-calli developed from cotyledon tissue of Norang-Bom cultivar successfully grew toward callus colonies on the solidified medium with 0.2 mg/L zeatin and 0.1 mM spermidine. The callus colonies generated de novo shoots at the maximum frequency of 4.3% on the medium with 5.0 mg/L BA and 1.0 mg/L NAA. Our results might be helpful for further studies to enhance the regeneration efficiency in Chinese cabbage protoplast culture.
참고문헌 (33)
- Bannerot H., "Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea", Proc EUCARPIA Meeting Cruciferae., pp.52~54 (1974)
- Bengochea T., "Plant protoplasts: A biotechnological tool for plant improvement" (1986), Chapman and Hall
- Chi G. L., "Role of ethylene on de novo shoot regeneration from cotyledonary explants of Brassica campestris ssp. Pekinensis (lour) Olsson in vitro", Physiol Plantarum, 96, pp.178~183 (1991)
- Curuk S., "Regeneration in vitro from the hypocotyls of Cucumis species produces almost exclusively diploid shoots, and does not require light", HortScience, 38, pp.105~109 (2003)
- Gamborg O. L., "Nutrient requirement of suspension cultures of soybean root cells", Exp Cell Res, 50, pp.151~158 (1968)
- Han E. J., "Root & shoot regeneration from the protoplast of Brassica campestris ssp. pekinensis", Korean J Plant Tiss Cult, 17, pp.285~289 (1990)
- Heyn F. W., "Cytoplasmic genetic male sterility in Brassica napus", EUCARPIA Cruciferae News 1, 3, pp.34~35 (1978)
- Huxter T. J., "Shooting initiation in light- and dark-grown tobacco callus: the role of ethylene", Physiol Plantarum, 53, pp.319~326 (1981)
- In D. S., "Regeneration of symmetric protoplast fusion between cabbage (Brassica oleracea L.) and radish (Raphanus sativus L.)", J Plant Biotechnol, 35, pp.121~126 (2008)
- Jain R. K., "Genotypic and media effects on plant regeneration from cotyledon explant cultures of some Brassica species", Plant Cell Tiss Org Cult, 14, pp.197~206 (1988)
- Jee S. O., "Protoplast culture of flower cabbage (Brassica oleracea L. var. acephala DC. forma tricolor Hort.), I. Factors affecting cell division in protoplast culture", J Kor Soc Hort Sci, 30, pp.96~107 (1989)
- Jee S. O., "Protoplast culture of flower cabbage (Brassica oleracea L. var. acephala DC. forma tricolor Hort.), II. Effect of plant growth regulators on plant regeneration", Korean J Plant Tiss Cult, 16, pp.15~24 (1989)
- Jee S. O., "Protoplast culture of flowering cabbage (Brassica oleracea L. var. acephala DC. forma tricolor Hort.), III. Plant regeneration from fused protoplasts", Korean J Plant Tiss Cult, 16, pp.79~92 (1989)
- Jourdan P. S., "Synthesis of male-sterile, triazine-resistant Brassica napus by somatic hybridization between cytoplasmic male-sterile B. oleracea and atrazine-resistant B. campestris", Theor Appl Genet, 78, pp.445~455 (1989)
- Kao K. N., "requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media", Planta, 126, pp.105~110 (1975)
- Kirti P. B., "Transfer of Ogu cytoplasmic male sterility to Brassica juncea and improvement of the male sterile line through somatic cell fusion", Theor Appl Genet, 91, pp.517~521 (1995)
- Kumar P. P., "The role of ethylene and carbon dioxide in differentiation of shoot buds in excised cotyledons of Pinus radiate in vitro", Physiol Plantarum, 69, pp.244~252 (1987)
- Leshem B., "Cytokinin and white light coact to enhance polypeptide metabolism and shoot regeneration in cultured melon cotyledons", J Plant Physiol, 145, pp.291~295 (1995)
- Lian YJ, "Production and characterization of somatic hybrids between Brassica campestris L. ssp pekinensis and Brassica oleracea L. var capitata", J Plant Biotechnol, 3, pp.33~38 (2001)
- Menczel L, "High frequency of fusion induced in freely suspended protoplast mixtures by polyethylene glycol and dimethylsulfoxide at high pH", Plant Cell Rep, 3, pp.196~198 (1984)
- Murashige T., "Revised medium for rapid growth and bioassays with tobacco tissue culture", Physiol Plantarum, 15, pp.473~497 (1962)
- Murata M., "Callus initiation and regeneration capacities in Brassica species", Plant Cell Tiss Org Cult, 11, pp.111~123 (1987)
- Narasimhulu S. B., "Species specific shoot regeneration response of cotyledonary explants of Brassicas", Plant Cell Rep, 7, pp.104~106 (1988)
- Nitch J.P ., "Haploid plants from pollen grains", Science, 163, pp.85~87 (1969)
- Ogura H, "Studies on the new male sterility in Japanese radish with special reference to utilization of this sterility toward the practical raising of hybrid seeds", Mem Fac Agric, 6, pp.39~78 (1968), Kagoshima Univ.
- Pelletier G., "Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion", Mol Gen Genet, 191, pp.244~250 (1983)
- Pierik R. L. M., "In vitro culture of higher plants" (1987), Martinus Nijhoff Pubishers
- Pua E. C., "Cellular and molecular aspects of ethylene on plant morphogenesis of recalcitrant Brassica species in vitro", Bot Bull Acad Sin, 34, pp.191~209 (1993)
- Ren J. P., "Improved resistance to bacterial soft rot by protoplast fusion between Brassica rapa and B. oleracea", Theor Appl Genet, 100, pp.810~819 (2000)
- Saha S., "Synergistic effect of kinetin and benzyl adenine plays a vital role in high frequency regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria) in relation to ethylene production", Breeding Sci, 57, pp.197~202 (2007)
- Sakai T., "Intergeneric transfer of cytoplasmic male sterility between Raphanus sativus (cms line) and Brassica napus through cytoplast-protoplast fusion", eor Appl Genet, 80, pp.421~427 (1990)
- Songstad D.D ., "Effect of 1-aminocyclopropane-1 carboxylic acid, silver nitrate and non-bornadiene on plant regeneration from maize callus cultures", Plant Cell Rep, 7, pp.262~265 (1988)
- Williams P. H., "Chinese Cabbage in : The origins and development of cytoplasmic male sterile Chinese cabbage", pp.293~300 (1981), Asian Vegetable Research and Development Center