Identification and characterization of QTLs and QTL interactions for Macro- and Micro-elements in rice (Oryza sativa L.) grain
2
조회
0
다운로드
0
인용
0
참고문헌
초록
Improvement of the macro- and micro-elements density of rice (Oryza sativa L.) is gradually becoming a new breeding objective. In this study, the genomic regions associated with potassium, calcium, magnesium and iron content in rice grain were identified and characterized by using a doubled haploid (DH) population. Fifty-six simple sequence repeat (SSR) and one hundred and twelve sequence tagged site (STS) markers were selected to construct the genetic linkage map of the DH population with a full length of 1808.3cM scanning 12 rice chromosomes. Quantitative trait loci (QTLs) were detected, and QTL effects and QTL interactions were calculated for five traits related to macro- and micro-elements in the DH population from a cross between ‘Samgang’ (Tongil) and ‘Nagdong’ (Japonica). Twelve QTLs were located on five chromosomes, consisting of two QTLs for potassium, three QTLs for calcium, two QTLs for magnesium, one QTL for iron content and four QTLs for the ratio of magnesium to potassium (Mg/K). Among them, qca1.1 was detected on chromosome 1 with an LOD value of 8.58 for calcium content. It explained 27% of phenotype variations with increasing effects from ‘Samgang’ allele. Furthermore, fifteen epistatic combinations with significant interactions were observed on ten chromosomes for five traits, which totally accounted for 4.19% to 12.72% of phenotype variations. The screening of relatively accurate QTLs will contribute to increase the efficiency of marker-assisted selection (MAS), and to accelerate the establishment of near-isogenic lines (NILs) and QTL pyramiding.
Abstract
Improvement of the macro- and micro-elements density of rice (Oryza sativa L.) is gradually becoming a new breeding objective. In this study, the genomic regions associated with potassium, calcium, magnesium and iron content in rice grain were identified and characterized by using a doubled haploid (DH) population. Fifty-six simple sequence repeat (SSR) and one hundred and twelve sequence tagged site (STS) markers were selected to construct the genetic linkage map of the DH population with a full length of 1808.3cM scanning 12 rice chromosomes. Quantitative trait loci (QTLs) were detected, and QTL effects and QTL interactions were calculated for five traits related to macro- and micro-elements in the DH population from a cross between ‘Samgang’ (Tongil) and ‘Nagdong’ (Japonica). Twelve QTLs were located on five chromosomes, consisting of two QTLs for potassium, three QTLs for calcium, two QTLs for magnesium, one QTL for iron content and four QTLs for the ratio of magnesium to potassium (Mg/K). Among them, qca1.1 was detected on chromosome 1 with an LOD value of 8.58 for calcium content. It explained 27% of phenotype variations with increasing effects from ‘Samgang’ allele. Furthermore, fifteen epistatic combinations with significant interactions were observed on ten chromosomes for five traits, which totally accounted for 4.19% to 12.72% of phenotype variations. The screening of relatively accurate QTLs will contribute to increase the efficiency of marker-assisted selection (MAS), and to accelerate the establishment of near-isogenic lines (NILs) and QTL pyramiding.
참고문헌 (16)
- Connolly E. L., "Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulation", Plant Cell, 14, pp.1347~1357 (2002)
- Hall J. L., "Transition metal transporters in plants", J Exp Bot, 54, pp.2601~2613 (2003)
- Graham R., "Breeding for micronutrient density in edible portions of staple food crops: conventional approaches", Field Crops Res, 60, pp.57~80 (1999)
- Ghandilyan A., "Progress in the genetic understanding of plant iron and zinc nutrition", Physiol Planta, 126, pp.407~417 (2006)
- Gregorio G. B., "Breeding for trace mineral density in rice", Food Nutr Bull, 21, pp.382~386 (2000)
- Joe Y. G., "QTL analysis of rice quality using a recombinant inbred population", J Agr Sci Chungbuk Nat’l Univ, 23, pp.147~164 (2006)
- Jung M. C., "Baseline study on essential and trace elements in polished rice from South Korea", Environ Geochem and Health, 27, pp.455~464 (2005)
- Kang H. J., "QTL mapping of genes related with grain chemical properties based on molecular map of rice", Korean J Crop Sci, 43, pp.199~204 (1998)
- Lander E. S., "MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations", Genomics, 1, pp.174~184 (1987)
- Phuong T. D., "Elemental content of Vietnamese rice Part1. Sampling, analysis and comprison with previous studies", Analyst, 124, pp.553~560 (1999)
- Salse J., "Synteny between Arabidopsis thaliana and rice at the genome level: a tool to identify conservation in the ongoing rice genome sequencing project", Nucleic Acids Research, 30, pp.2316~2328 (2002)
- Vreugdenhil D., "Natural variation and QTL analysis for cationic mineral content in seeds of Aradopsis thaliana", Plant Cell Environ, 27, pp.828~839 (2004)
- Wang D. L., "Mapping QTLs with epistatic and QTL x environmental interactions by mixed linear model approaches", Theor Appl Genet, 99, pp.1255~1264 (1999)
- Welch R. M., "Breeding for micronutrients in staple food crops from a human nutrition perspective", J Exp Bot, 55, pp.353~364 (2004)
- Yang T. J., "Construction of a linkage map in Capsicum annuum L. using RAPD markers and identification of two QTLs", J Plant Biotech, 1, pp.109~115 (1999)
- Zeng Z. B., "Precision mapping of quantitative trait loci", Genetics, 136, pp.1457~1468 (1994)