Quantitative trait loci controlling the amino acid content in rice (Oryza sativa L.)
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The amino acid composition of rice is a major concern of rice breeders because amino acids are among the most important nutrient components in rice. In this study, a genetic map was constructed with a population of 134 recombinant inbred lines (RILs) from a cross between Dasanbyeo (Tongil-type indica) and TR22183 (temperate japonica), as a means to detect the main and epistatic effect quantitative trait loci (QTLs) for the amino acid content (AAC). Using a linkage map which covered a total of 1458 cM based on 239 molecular marker loci, a total of six main-effect QTLs (M-QTLs) was identified for the content of six amino acids that were mapped onto chromosome 3. For all the M-QTLs, the TR22183 allele increased the trait values. The QTL cluster (flanked by id3015453 and id3016090) on chromosome 3 was associated with the content of five amino acids. The phenotypic variation, explained by the individual QTLs located in this cluster, ranged from 10.2 to 12.4%. In addition, 26 epistatic QTLs (Ep-QTLs) were detected and the 25 loci involved in this interaction were distributed on all nine chromosomes. Both the M-QTLs and Ep-QTLs detected in this study will be useful in breeding programs which target the development of rice with improved amino acid composition.
Abstract
The amino acid composition of rice is a major concern of rice breeders because amino acids are among the most important nutrient components in rice. In this study, a genetic map was constructed with a population of 134 recombinant inbred lines (RILs) from a cross between Dasanbyeo (Tongil-type indica) and TR22183 (temperate japonica), as a means to detect the main and epistatic effect quantitative trait loci (QTLs) for the amino acid content (AAC). Using a linkage map which covered a total of 1458 cM based on 239 molecular marker loci, a total of six main-effect QTLs (M-QTLs) was identified for the content of six amino acids that were mapped onto chromosome 3. For all the M-QTLs, the TR22183 allele increased the trait values. The QTL cluster (flanked by id3015453 and id3016090) on chromosome 3 was associated with the content of five amino acids. The phenotypic variation, explained by the individual QTLs located in this cluster, ranged from 10.2 to 12.4%. In addition, 26 epistatic QTLs (Ep-QTLs) were detected and the 25 loci involved in this interaction were distributed on all nine chromosomes. Both the M-QTLs and Ep-QTLs detected in this study will be useful in breeding programs which target the development of rice with improved amino acid composition.
참고문헌 (25)
- D’Mello JPF, "Amino Acids in Animal Nutrition", pp.1~14 (2003), CABI Publishing
- Duan M, "Profiling the expression of genes controlling rice grain quality", Plant Mol. Biol, 59, pp.165~178 (2005)
- Fan J, "Highly parallel SNP genotyping", Cold Spring Harb. Symp. Quant. Biol, 68, pp.69~78 (2003)
- Henderson JW, "Rapid, accurate, sensitive and reproducible HPLC analysis of amino acids", Agilent Technol. Tech, pp.1~10 (2000)
- Wenzhu Jiang, "Identification of QTLs for Seed Germination Capability after Various Storage Periods Using Two RIL Populations in Rice", Molecules and Cells, 31(4), pp.385~392 (2011), 한국분자세포생물학회
- Jiang XL, "Quantitative trait loci controlling amino acid contents in wheat (Triticum aestivum L.)", Aust. J. Crop Sci, 7, pp.820~829 (2013)
- Liu HY, "QTL analysis for gossypol and protein contents in upland cottonseeds with two different genetic systems across environments", Euphytica, 188, pp.453~463 (2012)
- Liu HY, "QTL mapping based on different genetic systems for essential amino acid contents in cottonseeds in different environments", PLoS One, 8, pp.e57531~ (2013)
- Lu K, "Genetic dissection of amino acid content in rice grain", J Sci Food Agric, 89, pp.2377~2382 (2009)
- Maclean JL, "Rice almanac", pp.6~7 (2002), CABI Publishing
- Mather KA, "The extent of linkage disequilibrium in rice (Oryza sativa L.)", Genetics, 177, pp.2223~2232 (2007)
- Meng L, "QTL IciMapping:integrated software for genetic linkage map construction and quantitative trait locus mapping in bi-parental populations", Crop J, 3, pp.265~279 (2015)
- Munck L, "Exploring the phenotypic expression of a regulatory proteome-altering gene by spectroscopy and chemometrics", Anal. Chim. Acta, 446, pp.171~186 (2001)
- Navea IP, "Identification of quantitative trait loci for panicle length and yield related traits under different water and P application conditions in tropical region in rice (Oryza sativa L.)", Euphytica, 213(2), pp.37~ (2017)
- Panthee DR, "Quantitative trait loci controlling sulfur containing amino acids, methionine and cysteine, in soybean seeds", Theor Appl Genet, 112, pp.546~553 (2006)
- Quampah A, "Mapping of quantitative trait loci for oil content in cotton seed kernel", J. Genet, 91, pp.289~295 (2012)
- Michael J. Thomson, "High-Throughput SNP Genotyping to Accelerate Crop Improvement", Plant Breeding and Biotechnology, 2(3), pp.195~212 (2014), 한국육종학회[DOI]
- Thomson MJ, "High-throughput single nucleotide polymorphism genotyping for breeding applications in rice using the BeadXpress platform", Mol. Breed, 29, pp.875~886 (2012)
- Wang LQ, "The QTL controlling amino acid content in grains of rice (Oryza sativa) are co-localized with the regions involved in the amino acid metabolism pathway", Mol. Breed, 21, pp.127~137 (2008)
- Wen J, "QTL mapping based on the embryo and maternal genetic systems for non-essential amino acids in rapeseed (Brassica napus L.) meal", J. Sci. Food Agric, 96, pp.465~473 (2016)
- Energy and protein requirements, "World Health Organization", World Health Organization, ~, pp.WHO Tech. Rep. Ser. 522 (1973)
- Wright MH, "ALCHEMY: a reliable method for automated SNP genotype calling for small batch sizes and highly homozygous populations", Bioinformatics, 26, pp.2952~2960 (2010)
- Zhao W, "Association analysis of the amino acid contents in rice", J. Integr. Plant Biol, 51, pp.1126~1137 (2009)
- Zheng X, "QTL analysis of maternal and endosperm genomes for Histidine and Arginine in rice (Oryza sativa L.) across environments", Acta. Agron. Sin, 34, pp.369~363 (2008)
- Zhou Y, "Overexpression of aspartate aminotransferase genes in rice resulted in altered nitrogen metabolism and increased amino acid content in seeds", Theor. Appl. Genet, 118, pp.1381~1390 (2009)