한지형 마늘에 있어서 기내뿌리로부터 식물체 재분화
Plant regeneration from callus derived root of northen type in garlic (Allium sativum L.)
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초록
본 연구는 마늘의 형질전환 체계를 확립하기 위해 캘 러스 형성과 식물체 재분화에 효과적인 방법을 개발하고 자 수행되었다. 가장 적합한 캘러스 형성 배지는 ‘단양 종’과 의성종 마늘 모두 1.0 mg/L 2,4-D 와 0.2 mg/L IAA 였다. 캘러스 형성율은 ‘단양종’ 마늘은 81.2% 였고 의성 종 마늘은 76.1%였다. 캘러스 형성을 유도한지 8주 후 형 성된 캘러스를 재분화 배지에서 7주 동안 배양하였다. 가 장 적합한 식물체 재분화 배지는 ‘단양종’ 마늘의 경우 5 mg/L kinetin와 1 mg/L NAA 였고 ‘의성종’ 마늘의 경우는 10 mg/L BAP였다. 재분화된 식물체는 하우스에 이식하 였는데, 거의 모두가 생존하였다. 기내뿌리를 이용한 식 물체 재분화 체계는 마늘 형질전환을 위해 유용하게 이 용 될 것이다.
Abstract
This study was conducted to develop an effective production of callus induction and plant regeneration system for garlic transformation. The best callus production occurred on in vitro root segment initially cultured on MS medium with 1.0 mg/L 2,4-D and 0.2 mg/L IAA in both ‘Danyang’ and ‘Euseong’. The frequency of callus formation were 81.2% ‘Danyang’ and 76.1% ‘Euseong’. Eight weeks after callus induction, callus lines were transferred to regeneration medium during 7 weeks. The best shoot regeneration medium was MS supplemented with 5 mg/L Kinetin and 1 mg/L NAA for ‘Danyang’ and MS supplemented with 10 mg/L BAP for ‘Euseong’. The frequency of shoot regeneration were 51.5% ‘Danyang’ and 56.6% ‘Euseong’ The plantlets were acclimatized and transferred to the greenhouse with almost survival. This in vitro regeneration system should be useful for garlic transformation.
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