국내콩 형질전환 기술개발
Development of genetic transformation method of Korean soybean
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그 동안 국내연구진에 의해서 발표된 논문을 중심으로 국내 콩형질전환기술의 개발현황을 알아보았다. 국내에 서 사용되고 있는 콩형질전환방법은 전 세계적으로 가장 보편적으로 사용되고 있는 자엽마디절에 아그로박테리 움을 이용하여 유전자를 도입하는 방법이 가장 높은 빈 도로 이용되었고, 그 외에 초음파방법, in planta방법, 그 리고 발아종자의 분열조직을 이용한 방법 등 비교적 다 양한 방법들이 사용되었다. 그러나 결과에 있어서는 재 현성이나 안정적인 형질전환의 효율이 외국의 사례에 비 해 높게 나오지는 않았다. 최근에 본 연구진에 의해서 개 발된 상대적으로 높은 형질전환결과를 바탕으로 고효율 형질전환기술을 위한 고려사항이 6가지 제안되었다. 제 안된 고려사항은 1) 형질전환 순응형 품종의 스크린 2) 정확한 부위로의 유전자 도입 3) 형질전환 직후에서부터 약 4주 정도에 이르는 기간 동안의 신초형성 4) 효율적인 선발조건 5) 효율적인 신초신장유도 그리고 6) 효율적인 발근유도이다
Abstract
Current status of soybean transformation method in Korera was reviewed with recent publications. Most frequently used method for genetic transformation was Agrobacterium-mediated transformation on cotyledonary node which is most popular method used in foreign country. In addition to this, various methods such as sonication- mediated transformation, in planta transformation, and transformation on meristem tissue of germinating seed, have been tried in Korea, even though their efficiencies on repeatability and stability were relatively low. Based on the promising results developed recently by reviewer, several important considerations for successful soybean transformations were suggested. They are 1) proper genotype screening, 2) targeting transformation on exact point, 3) multiple shoot formation, 4) efficient selection pressure, 5) successful shoot elongation, 6) efficient root formation. These are the basic requirements for stable and highly efficient soybean transformation of Korean soybean.
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