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콩 조직배양 기술에 기반한 생명공학 연구 동향

Status of Molecular Biotechnology Research Based on Tissue Culture of Soybean

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초록

콩은 전세계적으로 재배되는 중요한 작물 중에 하나로 최근, 표준유전체 해독과 함께 유전적, 표현형적으로 다양성을 가진한국핵심집단이 구축됨에 따라 유전체 기반 분자 육종 연구, 유전자 교정 기술을 활용한 새로운 육종 소재 개발 연구가 가속화될 것으로 예상된다. 유전체 정보 기반 작물의 분자 육종 및 생명공학 연구를 통한 성공적인 작물의 개량을 위해서는 식물의효율적인 조직배양 기술이 수반되어야 할 것이다. 그러나 반수체 생산, 원형질체 배양 및 형질전환 기술과 같은 콩의 조직배양효율은 아직까지 높지 않고 일부 계통에 한정되어 이루어지고있다. 본 논문에서는 콩의 분자육종 및 생명공학 기술의 적용을위하여 다양한 콩 조직배양 기술에 관한 연구 동향을 분석하고조직배양 효율에 영향을 미치는 요인들에 대한 정보를 제공하고자 하였다.

Abstract

Soybean (Glycine max (L.) Merrill) is one of the most important crops of the world. With the completion of the soybean genome sequence, the Korean soybean core collection consisted of 430 accessions with genetic and phenotypic diversity was constructed in recent year. The availability of genome sequences and core collection will result in the crop improvement by molecular breeding using the various accessions and genome editing approaches. Efficient tissue culture techniques, such as haploid production, protoplast culture and plant regeneration from various organs are essential for the successful molecular biological approach and crop improvement. However, soybean is still considered to be recalcitrant in tissue culture because of the low frequency of regeneration and limitation of available responsive cultivars. In this study, we discuss the recent studies of tissue culture technology and methodology for efficient tissue culture to genetic improvement and application of molecular biotechnology in soybean.

참고문헌 (129)

  1. plants at a high frequency, "Theor. Appl. Genet.", 2000, 1~6
  2. Bajaj, Y. P. S., "Interspecific hybridization of Brassica napus and B. juncea through ovary, ovule and embryo culture", Euphytica, 35, pp.103~109 (1986)
  3. Bao, A., "CRISPR", Cas9-mediated targeted mutagenesis of GmSPL9genes alters plant architecture in soybean, 2019, pp.19, 131~
  4. Barwale, U. B., "Screening of Glycine max and Glycine soja genotypes for multiple shoot formation at the cotyledonary node", Theor. Appl. Genet, 72, pp.423~428 (1986)
  5. Bezouw, R., "Converging phenomics and genomics to study natural variation in plant photosynthetic efficiency", Plant J, 97(1), pp.112~133 (2019)
  6. Blanca, J., "Transcriptome sequencing for SNP discovery across Cucumis melo", BMC Genomics, 13, pp.280~ (2012)
  7. Bolibok, H., "The identification of QTLs associated with the in vitro response of rye (Secale cereal L.)", Cell Mol. Biol. Lett., 12, pp.523~535 (2007)
  8. Buter, B., "Autoclaved and filter sterilized liquid media in maize anther culture: significance of activated charocoal", Plant Cell Rep, 13, pp.79~82 (1993)
  9. Cai, Y., "CRISPR", Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean, 2018, pp.16, 176~185
  10. Chen, L. P., "Plant regeneration from hypocotyl protoplasts of red cabbage (Brassica oleracea) by using nurse cultures", Plant Cell Tissue Org. Cult, 77, pp.133~138 (2004)
  11. seeds, "Plant Sci.", 2006, 399~406
  12. Chiera, J. M., "Ectopic expression of a soybean phytase in developing seeds of Glycine max to improve phosphorus availability", Plant Mol. Biol, 56, pp.895~904 (2004)
  13. 전재범, "변이밀집영역 유래 27개 InDel 마커를 이용한 콩(Glycine max (L.) Merrill) 신품종 판별 및 국내 149 품종과 유연관계 분석", 한국자원식물학회지, 32(5), pp.519~542 (2019), 한국자원식물학회[DOI]
  14. Cistué, L., "Production of doubled haploids in durum wheat (Triticum turgidum L.) through isolated microspore culture", Plant Cell Rep, 25, pp.257~264 (2006)
  15. Collonnier, U., "Somatic hybrids between Solanum melongena and S. sisymbrifolium, as a useful source of resistance against bacterial and fungal wilts", Plant Sci., 164, pp.849~861 (2003)
  16. Cunha, N. B., "Expression of functional recombinant human growth hormone in transgenic soybean seeds", Transgenic Res., 20, pp.811~826 (2011)
  17. Cunha, W. G., "High resistance to Sclerotinia sclerotiorum in transgenic soybean plants transformed to express an oxalate decarboxylate gene", Plant Pathol, 59, pp.654~660 (2010)
  18. Dan, Y., "Organogenic regeneration of soybean from hypocotyl explants", In Vitro Cell Dev. Biol. Plant, 34, pp.14~21 (1998)
  19. Dang, W., "An optimized Agrobacteriummediated transformation for soybean for expression of binary insect resistance genes", Plant Sci, 173, pp.381~389 (2007)
  20. Dhir, S. K., "Plantlet regeneration from immature cotyledon protoplasts of soybean (Glycine max L.)", Plant Cell Rep., 10(1), pp.39~43 (1991)
  21. Dhir, S. K., "Regeneration of fertile plants from protoplasts of soybean (Glycine max L. Merr.): Genotypic differences in culture response", Plant Cell Rep., 11(5-6), pp.285~289 (1992)
  22. Du, H., "Efficient targeted mutagenesis in soybean by TALENs and CRISPR", Cas9, 2016, pp.217, 90~97
  23. Dufourmantel, N., "Generation and analysis of soybean plastid transformants expressing Bacillus thuringiensis Cry1Ab protoxin", Plant Mol Biol., 58, pp.659~668 (2005)
  24. Dufourmantel, N., "Generation and characterization of soybean and marker-free tobacco plastid transformants over-expressing a bacterial 4-hydroxyphenylpyruvate dioxygenase which provides strong herbicide tolerance", Plant Biotech. J, 5, pp.118~133 (2007)
  25. Eck, J. V, "Genome editing and plant transformation of solanaceous food crops", Curr. Opin. Biotech, 49, pp.35~41 (2018)
  26. Eckert, H., "Co-expression of the borage Δ6 desaturase and the Arabidopsis Δ15 desaturase results in high accumulation of steridonic acid in the seeds of transgenic soybean", Planta, 224, pp.1050~1057 (2006)
  27. Farnham, M. W, "Doubled-haploid broccoli production using anther culture : effect of anther source and seed set characteristics of derived lines", J. Amer. Soc. Hort. Sci, 123, pp.73~77 (1998)
  28. Ferrie, A., "Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line", Plant Cell Rep., 14, pp.580~584 (1995)
  29. Finer, J. F., "Development of an embyrogenic suspension culture of soybean (Glycine max Merill)", Plant Cell Tissue Org. Cult, 15, pp.125~136 (1988)
  30. "Transgenic Res.", 2008, 839~850
  31. Gaj, M. D., "LEAFT COTYLEDON genes are essential for induction of somatic embryogenesis of Arabidopsis", Planta, 222, pp.977~988 (2005)
  32. Gamborg, O. L., "Somatic embryogenesis in cell cultures of Glycine species", Plant Cell Rep, 2, pp.209~212 (1983)
  33. Gazzarrini, S., "The transcription factor FUSCA3 controls development timing in Arabidopsis through the hormones gibberellin and abscisic acid", Dev. Cell, 7, pp.373~385 (2004)
  34. Ge, X. J., "A tissue culture system for different germplasms of indica rice", Plant Cell Rep, 25, pp.392~402 (2006)
  35. Guiderdoni, E., "Anther culture of tropical japonica x indica hybrids of rice (Oryza sativa L.)", Euphytica, 62(3), pp.219~224 (1992)
  36. Guo, W. W., "Targeted cybridization in citrus: transfer of Satuma cytoplasm to seedy cultivars for potential seedlessness", Plant Cell Rep, 22, pp.752~758 (2004)
  37. Hai, N. H., "Anther culture of Glycine max (Merr.): Effect of media on callus induction and organogenesis", Indian J. Genet., 76(3), pp.319~325 (2016)
  38. Hamada, H., "An in planta biolistic method for stable wheat transformation", Sci. Rep, 7, pp.11443~ (2017)
  39. Hammatt, A., "Isolation and culture of soybean hypocotyl protoplasts", In Vitro Cell. Develop. Biol, 24(6), pp.601~604 (1988)
  40. Hansen, G., "Recent advances in the transformation of plants", Trends in Plant Sci, 4(6), pp.226~231 (1999)
  41. He, G. Y., "Improvement of somatic embryogenesis and plant regeneration from durum wheat (Triticum turgidum var. durum Desf.) scutellum and inflorescence cultures", Euphytica, 119, pp.369~376 (2001)
  42. Hecht, V., "The Arabidopsis somatic embryogenesis receptor kinase 1 gene is expressed in developing ovules and embryos and enhances embryogenic competence in culture", Plant Physiol, 127, pp.803~816 (2001)
  43. Herman, E. M., "Genetic modification removes an immunodominant allergen from soybean", Plant Physiol, 132, pp.36~43 (2003)
  44. Hinchee, M., "Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer", Nat. Biotech, 6, pp.915~922 (1988)
  45. Ivers, D. R., "Anther culture in soybeans", Crop Sci, 14(6), pp.891~893 (1974)
  46. Ishida, Y, "Agrobacteriummediated transformation of maize", Nat. Protoc, 2, pp.1614~1621 (2007)
  47. Jacquard, C., "Microspore embryogenesis and programmed cell death in barley: effects of copper on albinism in recalcitrant cultivars", Plant Cell Rep, 28, pp.1329~1339 (2009)
  48. Jaganathan, D., "CRISPR for crop improvement: An update review", Front. Plant Sci, 9, pp.985~ (2018)
  49. Jeong, N., "Korean soybean core collection:Genotypic and phenotypic diversity population structure and genome-wide association study", PLoS ONE, 14(10), pp.e0224074~ (2019)
  50. Kakkar, R. K., "Polyamines and plant morphogenesis", Biol. Plant, 43, pp.1~11 (2000)
  51. Kaltchuk-Santos, E., "Cytological analysis of early microspore divisions and embryo formation in cultured soybean anthers", Plant Cell Tissue Org. Cult, 49(2), pp.107~115 (1997)
  52. Kaneda, Y., "Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybean (Glycine max (L.) Merrill)", Plant Cell Rep., 17, pp.8~12 (1997)
  53. "Biocatal. Agricult. Biotech.", 2019, 101173~
  54. Kao, K. N., "Cell division in newly formed cells from protoplasts of soybean", Exp. Cell Res, 62, pp.338~340 (1970)
  55. Kao, K. N., "Plant protoplast fusion and growth of intergeneric hybrid cells", Planta, 120(3), pp.215~227 (1974)
  56. Keller, W. A., "Production of haploids via anther culture in Brasica oleracea var", Italica. Euphytica, 32, pp.151~159 (1983)
  57. Khalafalla, M. M., "Optimization of particle bombardment conditions by monitoring of transient sGFP (S65T) expression in transformed soybean", Breed. Sci, 55, pp.257~263 (2005)
  58. 김동건, "기내 배양을 통한 국내 콩(Glycine max L.) 품종의 식물체 재분화", 한국자원식물학회지, 29(1), pp.143~153 (2016), 한국자원식물학회[DOI]
  59. 김혜정, "Overexpression of a Chromatin Architecture-Controlling ATPG7 has Positive Effect on Yield Components in Transgenic Soybean", Plant Breeding and Biotechnology, 5(3), pp.237~242 (2017), 한국육종학회[DOI]
  60. Kim, W. S., "Expression of an 11kDa methionine-rich delta-zein in transgenic soybean results in the formation of two types of novel protein bodies in transitional cells situated between the vascular tissue and storage parenchyma cells", Plant biotech. J., 2, pp.199~210 (2004)
  61. Kita, Y., "Genetic improvement of the somatic embryogenesis and regeneration in soybean and transformation of the improved breeding lines", Plant Cell Rep, 26, pp.439~447 (2007)
  62. Klein, A. S., "Cellulose and 1,3-glucan synthesis during the early stages of wall regeneration in soybean protoplasts", Planta, 152(2), pp.105~114 (1981)
  63. Komatsuda, T., "Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max", Theor. Appl. Genet, 75(5), pp.695~700 (1988)
  64. Lhee, W. Y., "Effect of BA and GA on embryo germination from ovule culture in intergeneric hybrids between Brassica and Raphanus", Korean J. Plant Tissue Cult, 24, pp.257~262 (1997)
  65. Li, R., "Vernonia DGATs increase accumulation of epoxy fatty acids in oil", Plant Biotech. J, 8, pp.184~195 (2010)
  66. Li, S., "Optimization of Agrobacterium-mediated transformation in soybean", Front Plant Sci, 24(8), pp.246~ (2017)
  67. Lin, C. S., "Application of protoplast technology to CRISPR", Cas9mutagenesis: From single-cell mutation detection to mutant plant regeneration, 2018, pp.16, 1295~1310
  68. Lin, W., "Soybean protoplast culture and direct gene uptake and expression by cultured soybean protoplasts", Plant Physiol, 84, pp.856~861 (1987)
  69. Mano, Y., "Identification of QTLs controlling tissue-culture traits in barley (Hordeum vulgare L.)", Theor. Appl. Genet, 105, pp.708~715 (2002)
  70. Matzke, A. J. M., "Homology-dependent gene silencing in transgenic plants: epistatic silencing loci contain multiple copies of methylated transgenes", Mol. Gen. Genet, 244, pp.219~229 (1994)
  71. McCabe, D. E., "Transformation of elite cotton cultivars via particle bombardment of meristems", Nature Biotech, 11, pp.596~598 (1993)
  72. Melchers, G., "Somatic hybridisation of plants by fusion of protoplasts", Mol. Gen. Genet, 135(4), pp.277~294 (1974)
  73. Neale D. B., "Decoding the massive genome of loblolly pine using haploid DNA and novel assembly strategies", Genome Biol., 15, pp.R59~ (2014)
  74. Newell, C. A., "Protoplast culture and plant regeneration in Glycine canescens F", J. Herm. Plant Cell Tissue Org. Cult, 4(2), pp.145~149 (1985)
  75. Nguyen, K. L., "Next-generation sequencing accelerates crop gene discovery", Trends Plant Sci, 24(3), pp.263~274 (2019)
  76. Nishimura, A., "Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems", PNASU, 102(33), pp.11940~11944 (2005)
  77. Ochatt, S., "Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae)", J. Plant Physiol, 166, pp.1314~1328 (2009)
  78. Parrott, W. A., "Effect of genotype on somatic embryogenesis from immature cotyledons of soybean", Plant Cell Tissue Org. Cult, 16, pp.15~21 (1989)
  79. Parrott, W. A., "Recovery and evaluation of soybean plants transgenic for a bacillus thuringiensis var. kurstaki insecticidal gene", In Vitro Cell. Dev. Biol., 30, pp.144~149 (1994)
  80. Phippen, C., "Genotype, plant, bud size and media factors affecting anther culture of cauliflowers (Brassica oleracea var. botrytis)", Theor. Appl. Genet., 79, pp.33~38 (1990)
  81. Qian, W., "Genetic effects on biomass yield in interspecific hybrids between Brassica napus and B. rapa", Euphytica, 134, pp.9~15 (2003)
  82. Rakshit, S., "Callus induction and whole plant regeneration in elite indian maize (Zea mays L.) inbreds", Plant Cell Tissue Org. Cult., 100, pp.31~37 (2009)
  83. Raza, G., "In vitro plant regeneration from commercial cultivars of soybean", BioMed Res. Int, 2017, pp.7379693~ (2017)
  84. Rhodes, C. A., "Genetically transformed maize plants from protoplast", Science, 240(4849), pp.204~207 (1988)
  85. Rodrigues, L. R., "Histology of embryogenic responses in soybean anther culture", Plant Cell Tissue Org. Cult, 80(2), pp.129~137 (2005)
  86. Sahoo, K. K., "An improved protocol for efficient transformation and regeneration of diverse indica rice cultivars", Plant Methods, 7, pp.49~ (2011)
  87. Sairam, R. V., "A study on effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus", Plant Cell Tissue Org. Cult., 75, pp.79~85 (2003)
  88. Sato, S., "Production of γ-linolenic acid and stearidonic acid in seeds of marker-free transgenic soybean", Crop. Sci, 44, pp.646~652 (2004)
  89. Schmutz J, "Genome sequence of the palaeopolyploid soybean", Nature, 463, pp.178~183 (2010)
  90. Seo, M. S., "Mapping quantitative trait loci for tissue culture response in VCS3MDH population of Brassica rapa", Plant Cell Rep, 32, pp.1251~1261 (2013)
  91. 서미숙, "배추 유전자원의 소포자 유래 배 발생 효율에 미치는 배양 조건 구명", Journal of Plant Biotechnology, 41(3), pp.116~122 (2014), 한국식물생명공학회
  92. 서미숙, "기내배양을 통한 Brassica 작물의 반수체 생산에 관한 연구 동향", 한국국제농업개발학회지, 27(4), pp.522~528 (2015), 한국국제농업개발학회[DOI]
  93. Shan, Z., "Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr)", Plant Cell Rep., 24(9), pp.507~512 (2005)
  94. Shetty, K., "Stimulation of in vitro shoot organogenesis in Glycine max (Merrill.) by allantoin and amides", Plant Sci., 81, pp.245~251 (1992)
  95. Singh, R. J., "Soybean genetic resources and crop improvement", Genome, 42, pp.605~616 (1999)
  96. Staswick, P. E., "Efficient down-regulation of the major vegetative storage protein genes in transgenic soybean does not compromise plant productivity", Plant Physiol, 127(4), pp.1819~1826 (2001)
  97. Sun, X., "Targeted mutagenesis in soybean using the CRISPRCas9system", Sci. Rep, 5, pp.10342~ (2015)
  98. Taguchi-Shiobara, F., "Mapping quantitative trait loci associated with regeneration ability of seed callus in rice, Oryza sativa L", Theor. Appl. Genet., 5, pp.828~833 (1997)
  99. Taguchi-Shiobara, F., "Mapping QTLs that control the performance of rice tissue culture and evaluation of derived near-isogenic lines", Theor. Appl. Genet, 112, pp.968~976 (2006)
  100. Takagi, K., "Manipulation of saponin biosynthesis by RNA interference-mediated silencing of β-amyrin synthase gene expression in soybean", Plant Cell Rep, 30, pp.1835~1846 (2011)
  101. Thakare, D., "The MADS-domain transcriptional regulator AGAMOUS-LIKE15promotes somatic embryo development in Arabidopsis and soybean", Plant Physiol, 146(4), pp.1663~1672 (2008)
  102. Tougou, M., "Soybean dwarf virus-resistant transgenic soybeans with the sense coat protein gene", Plant Cell Rep, 26, pp.1967~1975 (2007)
  103. Touraev, A., "Stress-induced microspore embryogenesis in tobacco : An optimized system for molecular studies", Plant Cell Rep, 15, pp.561~565 (1996)
  104. Travella, S., "A comparison of transgenic barley lines produced by particle bombardment and Agrobacteriummediated techniques", Plant Cell Rep, 23, pp.780~789 (2004)
  105. embryogenic suspension culture tissue, "Plant Cell Rep.", 1998, 482~488
  106. Uchimiya, H., "Expression of a foreign gene in callus derived from DNA treated protoplasts of rice (Oryza sativa L.)", Mol. Gen. Genet., 22, pp.421~477 (1986)
  107. Vagadia, B. H., "Inactivation methods of soybean trypsin inhibitor-a review", Trends Food Sci. Technol., 64, pp.115~125 (2017)
  108. Valente, M. A. S., "The ER luminal binding protein (BiP)mediates an increase in drought tolerance in soybean and delays drought-induced leaf senescence in soybean and tobacco", J. Exp. Bot, 60, pp.533~546 (2009)
  109. Vasil, I. K., "Transformation of wheat via particle bombardment", Meth. Mol. Biol, 318, pp.273~283 (2006)
  110. Velasco, R., "The genome of the domesticated apple (Malus × domestica Borkh.)", Nature Genetics, 42, pp.833~839 (2010)
  111. Wang, X., "Research progression soybean tissue culture system and transformation", Soybean Sci, 28, pp.731~735 (2009)
  112. Wei, Z. M., "Plant regeneration from protoplasts of soybean(Glycine max L", Plant Cell Rep, 7(5), pp.348~351 (1988)
  113. Wheatley, W. G., "Microspore growth and anther staging in wheat anther culture", Plant Cell Rep, 5, pp.47~49 (1986)
  114. Wright, M. S., "Plant regeneration by organogenesis in Glycine max", Plant Cell Rep, 5(2), pp.150~154 (1986)
  115. Chao Yang, "Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)", Molecules and Cells, 32(4), pp.337~342 (2011), 한국분자세포생물학회
  116. Yang, Y. S., "Comparative studies of organogenesis and plant regeneration in various soybean explants", Plant Sci, 72(1), pp.101~108 (1990)
  117. Yoshida, T, "Adventitious shoot formation from hypocotyl sections of mature soybean seeds", Breeding Sci., 52, pp.1~8 (2002)
  118. Yu, O., "Metabolic engineering to increase isoflavone biosynthesis in soybean seed", Phytochemistry, 63, pp.753~763 (2003)
  119. "Plant Cell Rep.", 2004, 478~482
  120. Zernova, O. V., "Modification of isoflavones in soybean seeds via expression of multiple phenolic biosynthetic genes", Plant Physiol. Biochem, 47, pp.769~777 (2009)
  121. Zieg, R. G., "The isolation, culture and callus formation of soybean pod protoplasts", Plant Sci. Lett., 18(2), pp.105~114 (1980)
  122. Zhang, C., "Functional analysis of the GmESR1 gene associated with soybean regeneration", Plos ONE, 12(4), pp.e0175656~ (2017)
  123. Zhang, K., "GmRAV1 regulates regeneration of roots and adventitious buds by the cytokinin signaling pathway in Arabidopsis and soybean", Physiol. Plant, 165(4), pp.814~829 (2018)
  124. Zhang, W., "Efficient regeneration of transgenic plants from rice protoplasts and correctly regulated expression of the foreign gene in the plants", Theor. Appl. Genet, 76, pp.835~840 (1998)
  125. Zhang, Y. X., "Microspore embryogenesis induced by low gamma dose irradiation in apple", Plant Breed, 108, pp.173~176 (1992)
  126. Zhao, G., "Germination of embryo in soybean anther culture", Chin. Sci. Bull, 43(23), pp.1991~1995 (1998)
  127. Zhao, Q., "5-azacytiding promotes shoot regeneration during Agrobacterium-mediated soybean transformation", Plant Physiol. Biochem, 141, pp.40~50 (2019)
  128. Zheng, Q., "Alterations in the transcriptome of soybean in response to enhanced somatic embryogenesis promoted by orthologs of Agamous-like15 and Agamouslike18", Plant Physiol, 164(3), pp.1365~1377 (2014)
  129. Zheng, Q., "Gene regulation by the AGL15 transcription factor reveals hormone interactions in somatic embryogenesis", Plant Physiol, 172(4), pp.2374~2387 (2016)

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