Antioxidant property of leaves and calluses extracts of in-vitro grown 5 different Ocimum species
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In this study, the antioxidant property of leaf and callus extracts of five selected in vitro grown Ocimum species (Ocimum sanctum, Ocimum kilimandscharicum, Ocimum gratissimum, Ocimum basilicum, and Ocimum americanum) and their respective callus extracts was investigated. The callus cultures were successfully initiated on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (1 mg･L) combined with different concentrations (0.1-0.4 mg･L) of kinetin as plant growth regulators. Total phenolic contents were estimated using the Folin-Ciocalteu reagent. 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power, Fe2+ chelating activity, and β-carotenelinoleic acid bleaching assays were used to determine the biological effects of the extracts. Interestingly, all the callus extracts exhibited significant (p<0.05) increase in phenolic contents and antioxidant activity. Furthermore, a liner correlation was obtained between the total phenolic contents and free radical scavenging activity (R2 = 0.783). The extracts of leaves and calluses of Ocimum species exhibited activity in all the in vitro antioxidant assays, but its extent was less potent that the positive controls butylated hydroxyl anisole (BHA) and ascorbic acid. A higher accumulation of phenolics in the callus extracts suggests that isolation of high-concentration materials with antioxidant activivity is possible from in vitro callus cultures rather than field-grown plant organs. Furthermore, these extracts may be used as an effective preservative in the food industry.
Abstract
In this study, the antioxidant property of leaf and callus extracts of five selected in vitro grown Ocimum species (Ocimum sanctum, Ocimum kilimandscharicum, Ocimum gratissimum, Ocimum basilicum, and Ocimum americanum) and their respective callus extracts was investigated. The callus cultures were successfully initiated on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) (1 mg･L) combined with different concentrations (0.1-0.4 mg･L) of kinetin as plant growth regulators. Total phenolic contents were estimated using the Folin-Ciocalteu reagent. 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging, ferric reducing antioxidant power, Fe2+ chelating activity, and β-carotenelinoleic acid bleaching assays were used to determine the biological effects of the extracts. Interestingly, all the callus extracts exhibited significant (p<0.05) increase in phenolic contents and antioxidant activity. Furthermore, a liner correlation was obtained between the total phenolic contents and free radical scavenging activity (R2 = 0.783). The extracts of leaves and calluses of Ocimum species exhibited activity in all the in vitro antioxidant assays, but its extent was less potent that the positive controls butylated hydroxyl anisole (BHA) and ascorbic acid. A higher accumulation of phenolics in the callus extracts suggests that isolation of high-concentration materials with antioxidant activivity is possible from in vitro callus cultures rather than field-grown plant organs. Furthermore, these extracts may be used as an effective preservative in the food industry.
참고문헌 (29)
- Benzie IFF, "The Ferric Reducing Ability of Plasma (FRAP) as a Measure of “antioxidant Power”", The FRAP Assay Analytical Biochemistry, 239, pp.70~76 (1996)
- Chatterjee A, "Nematicidal principles from two species of lamiaceae", J Nematol, 14, pp.118~120 (1982)
- Deshpande RS, "Insecticidal activity of Ocimum basilicum Linn", Pesticides, 11, pp.11~12 (1977)
- Dinis TCP, "Action of Phenolic Derivatives (Acetaminophen, Salicylate, and 5- Aminosalicylate) as Inhibitors of Membrane Lipid Peroxidation and as Peroxyl Radical Scavengers", Archives of Biochemistry and Biophysics, 315, pp.161~169 (1994)
- Dorman HJD, "Characterisation of the antioxidant properties of de-Jodourised aqueous extracts from selected Lamiaceae herbs", Food Chemistry, 83, pp.255~262 (2003)
- Gyamfi MA, "Free-radical scavenging action of medicinal herbs from Ghana: Thonningia sanguinea on experimentally-induced liver injuries", General Pharmacology: The Vascular System, 32, pp.661~667 (1999)
- Hakkim FL, "Antioxidant property of selected Ocimum species and their secondary metabolite content", Journal of Medicinal Plants Research, 2, pp.250~257 (2008)
- Hakkim FL, "Chemical Composition and Antioxidant Property of Holy Basil (Ocimum sanctum L.) Leaves, Stems, and Inflorescence and Their in Vitro Callus Cultures", Journal of Agricultural and Food Chemistry, 55, pp.9109~9117 (2007)
- Halliwell B, "Oxygen toxicity, oxygen radicals, transition metals and disease", Biochem J, 219, pp.1~14 (1984)
- Hirasa K, "Spice Science Technology" (1998), Dekker M
- Kaur H, "The free radical chemistry of food additives", pp.17~35 (1991), Taylor and Francis Free Radicals and Food Additives
- Koleva II, "Screening of Plant Extracts for Antioxidant Activity: aComparative Study on Three Testing Methods", PhytochemicalAnalysis, 13, pp.8~17 (2002)
- Liyana-Pathirana CM, "Antioxidant properties of commercial soft and hard winter wheats (Triticum aestivum L.) and their milling fractions", Journal of the Science of Food and Agriculture, 86, pp.477~485 (2006)
- Lutomski J., "Znaczenie ziolw terapii i dietetyce", Herba polonica, 48, pp.300~310 (2002)
- Mahoney JR, "Role of Alpha-Tocopherol, Ascorbic Acid, Citric Acidand EDTA as Oxidants in Model Systems", Journal of Food Science, 51, pp.1293~1296 (1986)
- Miller, Denis, "Food Chemistry: A Laboratory Manual", pp.153~ (1998), Wiley
- Murashige T, "A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures", Physiologia Plantarum, 15, pp.473~497 (1962)
- Ozkan G, "Inhibition of Pathogenic Bacteria by Essential Oils at Different Concentrations", Food Science and Technology International, 9(2), pp.85~88 (2003)
- Paula C, "Phenolicantioxidant compounds produced by in vitroshoots of sage (Salvia officinalis L.)", Plant Sci, 162, pp.981~ (2002)
- Porter WL, "Recent trends in food applications of antioxidants", pp.295~365 (1986), Plenum Press Autoxidation in Food and Biological Systems
- Ramarathnam N, "Antioxidant defense system in vegetable extracts", pp.76~ (1997), Japan Natural Antioxidants; Chemistry, Health Effects and Application
- Simon JE, "Basil: A Source of Aroma Compounds and a Popular Culinary and Ornamental Herb" (1999), Perspectives on new crops and new uses
- Singleton VL, "Analysis of total phenols and other oxidationsubstrates and antioxidants by means of folin-ciocalteureagent", Methods in Enzymology Academic Press, 299, pp.152~178 (1999)
- Szabo E, "Fungal elicitor preparations and methyl jasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumei", Plant Cell Rep, 18, pp.485~ (1999)
- Szeto YT, "Total antioxidant and ascorbic acid content of fresh fruits and vegetables: implications for dietary planning and food preservation", Br J Nutr, 87, pp.55~59 (2002)
- Vadivel V, "Effect of certain indigenous processing methods on the bioactive compounds of ten different wild type legume grains", Journal of Food Science and Technology, pp.1~12 (2011)
- Velioglu YS, "Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products", J Agric Food Chem, 46, pp.4113~ (1998)
- Verpoorte R, "Metabolic Engineering of Plant Secondary Metabolism" (2000), Alferman AW Dordrecht Netherlands Kluwer Academic press
- Yamaguchi F, "Antioxidative and Anti-Glycation Activity of Garcinol from Garcinia indica Fruit Rind", Journal of Agricultural and Food Chemistry, 48, pp.180~185 (2000)