Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims)
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Purple passion fruit (Passiflora edulis Sims) is one of the introduced tropical plants, an increasing interest has arisen due to its distinctive taste and attractive flavor. It is expected that passion fruit production and planted area will increase gradually in the years ahead because of high profitability and consumer’s demands of healthful ingredients. So we tried to investigate the effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of passion fruit for an establishment of optimal mass propagation system. Young leaf explants of purple passion fruit were cultured in Murashige and Skoog (MS) medium containing different growth regulators and antioxidant additives to induce the shoot organogenesis. After 8 weeks, the highest embryogenic callus formation rate was obtained in MS medium supplemented with 1 mg･L -1 6-benzylaminopurine (BAP) and 2 mg･L -1 2,4-dichlorophenoxyacetic acid (2,4-D), furthermore, the shoot development via organogenesis was also observed. Silver nitrate (AgNO3), which was added into the medium to minimize the adverse effects of leached phenolics, was effective for reduction of medium browning and sudden explant death. In the medium supplemented with 1 mg･L -1 BAP and 1 mg･L -1 gibberellic acid (GA3), shoots were most vigorously regenerated and elongated. Most shoots rooted successfully in half strength medium with 1 mg･L -1 indol-3 acetic acid (IAA), and more than 90% of plantlets survived after 4-month acclimatization period.
Abstract
Purple passion fruit (Passiflora edulis Sims) is one of the introduced tropical plants, an increasing interest has arisen due to its distinctive taste and attractive flavor. It is expected that passion fruit production and planted area will increase gradually in the years ahead because of high profitability and consumer’s demands of healthful ingredients. So we tried to investigate the effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of passion fruit for an establishment of optimal mass propagation system. Young leaf explants of purple passion fruit were cultured in Murashige and Skoog (MS) medium containing different growth regulators and antioxidant additives to induce the shoot organogenesis. After 8 weeks, the highest embryogenic callus formation rate was obtained in MS medium supplemented with 1 mg･L -1 6-benzylaminopurine (BAP) and 2 mg･L -1 2,4-dichlorophenoxyacetic acid (2,4-D), furthermore, the shoot development via organogenesis was also observed. Silver nitrate (AgNO3), which was added into the medium to minimize the adverse effects of leached phenolics, was effective for reduction of medium browning and sudden explant death. In the medium supplemented with 1 mg･L -1 BAP and 1 mg･L -1 gibberellic acid (GA3), shoots were most vigorously regenerated and elongated. Most shoots rooted successfully in half strength medium with 1 mg･L -1 indol-3 acetic acid (IAA), and more than 90% of plantlets survived after 4-month acclimatization period.
참고문헌 (45)
- Arditti J, "Micropropagation of orchids", pp.373~375 (1993), John Wiley and Sons
- Arnaldos TL, "Changes in phenol content during strawberry (Fragaria x ananasa, cv. Chandler) callus culture", Physiol Plant, 113, pp.315~322 (2001)
- Bais HP, "Influence of polyamines on growth of hairy root cultures of witloof chiocory (Chichorium intybus L cv Lucknow local) and formation of coumarins", J Plant Growth Regul, 18, pp.33~37 (1999)
- Bais HP, "AgNO3influences in vitro root formation in Decalepis hamiltonii Wight and Arn", Current Science, 79, pp.894~898 (2000)
- Bais, HP, "Putrescine and AgNO3 influences shoot multiplication In vitro flowering and endogenous titers of polyamines in Chichorium intybus L. cv. Lucknow Local", J Plant Growth Regul, 19, pp.238~248 (2000)
- Bais HP, "Influence of putrescine AgNO3 and polyamine inhibitors on the morphogenetic response in untransformed and transformed tissues of Chichorium intybus and their regenerants", Plant Cell Reports, 20, pp.547~555 (2001)
- Bais HP, "Putrescine influences growth and production of coumarins in transformed and untransformed root cultures of witloof chicory (Chichorium intybus L. cv. Lucknow Local)", Acta Physiologia Plantarum, 23, pp.319~327 (2001)
- Bais HP, "Agrobacterium rhizogenes-mediated transformation of Witloof chicory–in vitro shoot regeneration and induction of flowering", Current Science, 80, pp.83~87 (2001)
- Becerra DC, "Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa", Plant Cell Tiss Org Cult, 79, pp.87~90 (2004)
- Beyer EM, "Silver ion: a potent anti-ethylene agent in cucumber and tomato", HortScience, 11, pp.175~196 (1976)
- Braglia L, "In vitro plant regeneration as a tool to improve ornamental characters in Passiflora species", Acta Hort, 855, pp.47~52 (2010)
- De Klerk GJ, "Regeneration of roots, shoots and embryos: physiological, biochemical and molecular aspects", Biol Plant, 39, pp.53~66 (1997)
- Dornelas MC, "Tissue culture studies on species of Passiflora", Plant Cell Tiss Org Cult, 36, pp.211~217 (1994)
- Drew RA, "In vitro culture of adult and juvenile bud explants of Passiflora species", Plant Cell Tiss Organ Cult, 26, pp.23~27 (1991)
- Duclercq J, "De novo shoot organogenesis: from art to science", Trends Plant Sci, 16, pp.597~606 (2011)
- Faria JLC, "Micropropagation of yellow passionfruit by axillary bud proliferation", Hortscience, 32, pp.1276~1277 (1997)
- Fehér A, "Transition of somatic plant cells to an embryogenic state", Plant Cell Tiss Organ Cult, 74, pp.201~228 (2003)
- Fernando JA, "New insights into the in vitro organogenesis process:the case of Passiflora", Plant Cell Tiss Organ Cult, 91, pp.37~44 (2007)
- Gaj MD, "Factors influencing somatic embryogenesis induction and plant regeneration with particular reference to Arabidopsis thaliana (L.) Heynh", Plant Growth Regul, 43, pp.27~47 (2004)
- Gao GX, "Browning in plant tissue culture", Plant Physiol Commun, 35, pp.501~506 (1999)
- Garcia R, "Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae)", Plant Cell Tissue Organ Cult, 106, pp.47~54 (2011)
- Gordon SP, "Pattern formation during de novo assembly of the Arabidopsis shoot meristem", Development, 134, pp.3539~3548 (2007)
- Haensch KT, "Influence of 2,4-D and BAP on callus growth and the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum cv. Madame Layal", Electronic Journal of Biotechnology, 10, pp.1~9 (2007)
- Hall RM, "Efficient organogenesis of an Australian passionfruit hybrid (Passiflora edulis x Passiflora edulis var. flavicarpa) suitable for gene delivery", Aust J Bot, 48, pp.673~680 (2000)
- Jiménez VM, "Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis", J Plant Growth Regul, 47, pp.91~110 (2005)
- Kawata K, "Micropropagation of passion fruit from subcultured multiple shoot primordia", J Plant Physiol, 147, pp.281~284 (1995)
- Kumar V, "AgNO3: a potential regulator of ethylene activity and plant growth modulator", Electronic Journal of Biotechnology, 12, pp.8~9 (2009)
- Lainé E, "Regeneration of plants from leaf explants of micropropagated clonal Eucalyptus grandis", Plant Cell Rep, 13, pp.473~476 (1994)
- Lombardi SP, "In vitro shoot regeneration from roots and leaf discs of Passiflora cincinnata mast", Braz Arch Biol Technol, 50, pp.239~247 (2007)
- Nhut DT, "Protocols for Micropropagation of Woody Trees and Fruits", pp.417~426 (2007), Springer Netherlands
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Pacheco G, "Plant regeneration, callus induction and establishment of cell suspension cultures of Passiflora alata Curtis", Sci. Hortic, 144, pp.42~47 (2012)
- Paim-Pinto DL, "Somatic embryogenesis from mature zygotic embryos of commercial passionfruit (Passiflora edulis Sims)genotypes", Plant Cell Tiss Organ Cult, 107, pp.521~530 (2011)
- Pereira AMS, "Micropropagation of Pothomorphe umbellata via direct organogenesis from leaf explants", Plant Cell Tiss Organ Cult, 60, pp.47~53 (2000)
- Pinto AP, "In vitro organogenesis of Passiflora alata", vitro Cell Dev Biol Plant, 46, pp.28~33 (2010)
- Rathore TS, "In vitro regeneration of Pitcher plant (Nepenthes khasiana Hook. F.)- A rare insectivorous plant of India", J Plant Physiol, 139, pp.246~248 (1991)
- Rodriguez MV, "Cultivo in vitro de Passiflora alata, una forma de conservacíon genética", Actas de Horticultura, 48, pp.69~72 (2007)
- Sanyal I, "Agrobacterium mediated transformation of chickpea (Cicer arietinum L.)with Bacillus thuringiensis cry1Ac gene for resistance against pod borer insect Helicoverpa armigera", Plant Sci, 168, pp.1135~1146 (2005)
- Silva CV, "Organogenesis from root explants of commercial populations of Passiflora edulis Sims and a wild passionfruit species, P. cincinnata Masters", Plant Cell Tiss Organ Cult, 107, pp.407~416 (2011)
- Trevisan F, "Optimization of in vitro organogenesis in passion fruit (Passiflora edulis f. flavicarpa)", Sci Agric, 62, pp.346~350 (2005)
- Rajabpoor Sh, "Effects of different concentrations of 2,4-D and BAP on somatic embryogenesis induction in saffron (Crocus sativus L.)", Pak J Biol Sci, 10, pp.3927~3930 (2007)
- Rocha DI, "Alternative induction of de novo shoot organogenesis or somatic embryogenesis from in vitro cultures of mature zygotic embryos of passion fruit (Passiflora edulis Sims) is modulated by the ratio between auxin and cytokinin in the medium", Plant Cell Tiss Organ Cult, 120, pp.1087~1098 (2015)
- Yang X, "Regulation of somatic embryogenesis in higher plants", Crit Rev Plant Sci, 29(1), pp.36~57 (2010)
- Zhang SH, "Factors influencing the browning of potato mesophyll protoplasts and the effect of AgNO3 on their browning and division", China Potato J, 18, pp.77~81 (2004)
- Zhou JH, "Advance of studies on browning and antibrowning techniques in the tissue culture of horticultural plants", Acta Hortic J, 27, pp.481~486 (2000)