Expression of Dengue virus EIII domain-coding gene in maize as an edible vaccine candidate
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Plant-based vaccines possess some advantagesover other types of vaccine biotechnology such as safety,low cost of mass vaccination programs, and wider use ofvaccines for medicine. This study was undertaken to developthe transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculatedwith A. tumefaciens strain C58C1 containing the binaryvectors (V662 or V663). The vectors carrying nptII gene asselection marker and scEDIII (V662) or wCTB-scEDIII(V663) target gene, which code EIII proteins inhibite viraladsorption by cells. In total, 721 maize immature embryoswere transformed and twenty-two putative transgenic plantswere regenerated after 12 weeks selection regime. Of them,two- and six-plants were proved to be integrated with scEDIIIand wCTB-scEDIII genes, respectively, by Southern blotanalysis. However, only one plant (V662-29-3864) canexpress the gene of interest confirmed by Northern blotanalysis. These results demonstrated that this plant could beused as a candidated source of the vaccine production.
Abstract
Plant-based vaccines possess some advantagesover other types of vaccine biotechnology such as safety,low cost of mass vaccination programs, and wider use ofvaccines for medicine. This study was undertaken to developthe transgenic maize as edible vaccine candidates for humans. The immature embryos of HiII genotype were inoculatedwith A. tumefaciens strain C58C1 containing the binaryvectors (V662 or V663). The vectors carrying nptII gene asselection marker and scEDIII (V662) or wCTB-scEDIII(V663) target gene, which code EIII proteins inhibite viraladsorption by cells. In total, 721 maize immature embryoswere transformed and twenty-two putative transgenic plantswere regenerated after 12 weeks selection regime. Of them,two- and six-plants were proved to be integrated with scEDIIIand wCTB-scEDIII genes, respectively, by Southern blotanalysis. However, only one plant (V662-29-3864) canexpress the gene of interest confirmed by Northern blotanalysis. These results demonstrated that this plant could beused as a candidated source of the vaccine production.
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