장기간 계대배양 된 장미 배발생 캘러스로부터 식물체 재분화 및 비정형체로부터 새로운 배발생캘러스 재생
New embryogenesis from atypical bodies and plant regeneration from long-term subcultured embryogenic callus in rose
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초록
Long-term subcultured rose embryogenic calluses,which had been maintained for more than 5 to 6 years sincethe first embryogenesis from calluses induced from in vitroroots of rose, were identified as potential material for thedevelopment of transgenic plants. The first embryogeniccalluses from ‘Sweet Yellow’ and two breeding lines (KR056002and KR056006) were obtained in 2007 and 2009, respectively. Subsequently, we found that plants regenerated from longtermembryogenic calluses (LEC). Whereas the LEC from‘Sweet Yellow’ takes 3 to 4 months to regenerate plants,those of the two breeding lines take 4 to 5 months. Thisperiod of time is the same as that taken for plants to regeneratefrom the first embryogenic callus. New embryogenesis wasobserved from atypical bodies (ABs) that appeared duringthe process of long-term subculture. We found that it ispossible to use the AB as a material for new embryogenesis.
Abstract
Long-term subcultured rose embryogenic calluses,which had been maintained for more than 5 to 6 years sincethe first embryogenesis from calluses induced from in vitroroots of rose, were identified as potential material for thedevelopment of transgenic plants. The first embryogeniccalluses from ‘Sweet Yellow’ and two breeding lines (KR056002and KR056006) were obtained in 2007 and 2009, respectively. Subsequently, we found that plants regenerated from longtermembryogenic calluses (LEC). Whereas the LEC from‘Sweet Yellow’ takes 3 to 4 months to regenerate plants,those of the two breeding lines take 4 to 5 months. Thisperiod of time is the same as that taken for plants to regeneratefrom the first embryogenic callus. New embryogenesis wasobserved from atypical bodies (ABs) that appeared duringthe process of long-term subculture. We found that it ispossible to use the AB as a material for new embryogenesis.
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