RAW264.7 대식세포에서 풀솜대 추출물의 nitric oxide 및prostaglandin E2생성 저해효과
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초록
Smilacina japonica is a localized commonrhizomatous flowering plant, This plant is often used inKorean traditional systems of medicine as a remedy formigrain, diplegia, physical impurity, blood circulation,abscess and contusion. Generally drugs that are used forarthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has notbeen scientifically investigated so far. Therefore, the aim ofthis study was to investigate the anti-inflammatory potentialof S. japonica using the ethanolic extract and its subfractions. To evaluate the anti-inflammatory effects, weexamined the inflammatory mediators such as nitric oxide(NO) and prostaglandin E2 (PGE2) on RAW 264.7macrophages. Our results indicated that hexane fractionsignificantly inhibited the LPS induced NO and PGE2production in the cells. The hexane fractions inhibitoryactivity for NO tests with IC50 values showed in 53.3 μg/mland PGE2 tests with IC50 values showed at 32.5 μg/ml. Theseis result suggest a potential role of hexane fractionfrom S. japonica as source of anti-inflammatory agent.
Abstract
Smilacina japonica is a localized commonrhizomatous flowering plant, This plant is often used inKorean traditional systems of medicine as a remedy formigrain, diplegia, physical impurity, blood circulation,abscess and contusion. Generally drugs that are used forarthritis have antinociceptive and anti-inflammatory properties. However, validity of the anti-inflammatory activity has notbeen scientifically investigated so far. Therefore, the aim ofthis study was to investigate the anti-inflammatory potentialof S. japonica using the ethanolic extract and its subfractions. To evaluate the anti-inflammatory effects, weexamined the inflammatory mediators such as nitric oxide(NO) and prostaglandin E2 (PGE2) on RAW 264.7macrophages. Our results indicated that hexane fractionsignificantly inhibited the LPS induced NO and PGE2production in the cells. The hexane fractions inhibitoryactivity for NO tests with IC50 values showed in 53.3 μg/mland PGE2 tests with IC50 values showed at 32.5 μg/ml. Theseis result suggest a potential role of hexane fractionfrom S. japonica as source of anti-inflammatory agent.
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