土茯苓의 Raw 264.7 세포에 대한 항염효과
Anti-inflammatory Effects of Smilacis Glabrae Rhizoma in Raw 264.7 Cells
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Objective : Inflammatory cytokines have a close relationship to insulin dependent diabetes mellitus (IDDM). The inhibitory effect of Smilacis Glabrae Rhizoma (SGR) were examined on production of nitric oxide (NO), prostaglandin E2 (PGE2), synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and NF-κB activation in Raw 264.7 cells. Methods : Raw 264.7 cells were pretreated with SGR(20, 50, 100 ㎍/㎖), and then cultured with lipopolysaccharides (LPS). Cell viability was measured by MTT assay; inhibition of NO, PGE2, and TNF-α production were measured by Griess reagent and enzyme-linked immunosorbent assay(ELISA). Induction of COX-2 and iNOS were determined by western blotting analysis. Inhibition of NF-κB was measured by immunofluorescence assay (IFA). Results : SGR inactivated NF-κB, and inhibited the production of NO, iNOS, and PGE2. Inhibition of COX-2 and TNF-α could not be confirmed. Conclusions : From the above result, SGR was found to have an anti-inflammatory effect of inhibition of NO, iNOS, and PGE2 production via inhibition of NF-κB.
Abstract
Objective : Inflammatory cytokines have a close relationship to insulin dependent diabetes mellitus (IDDM). The inhibitory effect of Smilacis Glabrae Rhizoma (SGR) were examined on production of nitric oxide (NO), prostaglandin E2 (PGE2), synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and NF-κB activation in Raw 264.7 cells. Methods : Raw 264.7 cells were pretreated with SGR(20, 50, 100 ㎍/㎖), and then cultured with lipopolysaccharides (LPS). Cell viability was measured by MTT assay; inhibition of NO, PGE2, and TNF-α production were measured by Griess reagent and enzyme-linked immunosorbent assay(ELISA). Induction of COX-2 and iNOS were determined by western blotting analysis. Inhibition of NF-κB was measured by immunofluorescence assay (IFA). Results : SGR inactivated NF-κB, and inhibited the production of NO, iNOS, and PGE2. Inhibition of COX-2 and TNF-α could not be confirmed. Conclusions : From the above result, SGR was found to have an anti-inflammatory effect of inhibition of NO, iNOS, and PGE2 production via inhibition of NF-κB.
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