Initiation of embryogenic callus from mature zygotic embryos in Japanese larch (Larix kaempferi)
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Embryogenic callus (EC) was created from mature embryos of Larix kaempferi. With the mature embryos, keeping the culture in dark conditions throughout the experiment (38.2%) seemed to give better results than exposing them to 16 h light (25 μEm -2 s -1 ) for the first week (21.9%). EC was obtained most frequently from Quoirin and Lepoivre (LP) mediums with 1.0 mg/L 4-amino-3,5,6- trichloropicolinic acid (Picloram), plus 1.0 mg/L benzyladenine (BA) (62.8%) or Litvay's medium (LM) containing 1.0 mg/L p-chlorophenoxyacetic acid (pCPA) plus 1.0 mg/L BA (62.8%) treatment. In both cases, best results were obtained when zygotic embryos were cultured in darkness. As for the effective sucrose concentration on initiation of EC, 29.2 mM sucrose (38.6%) gave the best results.
Abstract
Embryogenic callus (EC) was created from mature embryos of Larix kaempferi. With the mature embryos, keeping the culture in dark conditions throughout the experiment (38.2%) seemed to give better results than exposing them to 16 h light (25 μEm -2 s -1 ) for the first week (21.9%). EC was obtained most frequently from Quoirin and Lepoivre (LP) mediums with 1.0 mg/L 4-amino-3,5,6- trichloropicolinic acid (Picloram), plus 1.0 mg/L benzyladenine (BA) (62.8%) or Litvay's medium (LM) containing 1.0 mg/L p-chlorophenoxyacetic acid (pCPA) plus 1.0 mg/L BA (62.8%) treatment. In both cases, best results were obtained when zygotic embryos were cultured in darkness. As for the effective sucrose concentration on initiation of EC, 29.2 mM sucrose (38.6%) gave the best results.
참고문헌 (24)
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