Propagation by In Vitro Zygotic Embryos Cultures of the Quercus myrsinifolia
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초록
Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA3 on the growth of plants germinated from zygotic embryos through GA3 treatment, the best growth was shown in 0.5 mg/l GA3 treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.
Abstract
Zygotic embryo culture was performed to propagate evergreen oak, Quercus myrsinifolia, which has recalcitrant seeds and is difficult to propagate by cuttings. Zygotic embryos appeared in WPM medium after 14 days, and after 56 days, they developed into complete plants with cotyledons and roots. The medium suitable for zygotic embryo culture was 1/4 WPM medium, showing a shoot growth of 2.43 cm and root growth of 8.7 cm after 8 weeks of culture. As a result of investigating the effect of GA3 on the growth of plants germinated from zygotic embryos through GA3 treatment, the best growth was shown in 0.5 mg/l GA3 treatment. The in vitro rooting and growth of IBA-treated zygotic embryo-derived plants were good in the 0.5 mg/l IBA treatment and rooting and shoot growth were not observed at higher concentrations. And the callus induction rate also increased as the concentration of IBA increased. Plants grown in vitro were transferred to a plastic pot containing artificial soil and acclimatized in a greenhouse for about 4 weeks, resulting in more than 90% survival. As a result of this study, the zygotic embryo culture method was confirmed to be effective for mass propagation of Q. myrsinifolia. The results of this study are expected to contribute significantly to the mass propagation of elite Q. myrsinifolia.
참고문헌 (27)
- Bonner FT, "Responses to Drying of Recalcitrant Seeds of Quercus nigra L", Ann Bot, 78, pp.181~187 (1996)
- Chalupa V, "In vitro propagation of oak(Quereus robur L. )and linden(Tilia cordata Mill. )", Biol Plant, 26, pp.374~377 (1984)
- Cornea-Cipcigan M, "Gibberellic Acid Can Improve Seed Germination and Ornamental Quality of Selected Cyclamen Species Grown Under Short and Long Days", Agronomy, 10, pp.516~ (2020)
- Dohling S, "Optimization of nutrient requirements for asymbiotic seed germination of Dendrobium longicornu and D. formosum Roxb", Proc Indian Natl Sci Acad, 74, pp.167~171 (2008)
- Frick EM, "Roles for IBA-derived auxin in plant development", J Exp Bot, 69, pp.169~177 (2018)
- Hanyan X, "Effects of GA_3 on the Seed Germination of Cyclamen Persicum", Seed, 4, pp.62~ (2006)
- Koene FM, "Asymbiotic seed germination and in vitro seedling development of Acianthera prolifera(Orchidaceae)", S Afr J Bot, 121, pp.83~91 (2019)
- "Forest Resources" (2018)[URL]
- Kumar R, "Effect of GA3 and ethrel on growth and flowering of African marigold cv. Pusa Narangi Gainda", Indian J Hortic, 67, pp.362~366 (2010)
- Lewis S, "Avoiding Dangerous Climate Change", pp.147~153 (2006), Cambridge University Press
- Lloyd G, "Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture", Proc Int Plant Propag Soc, 30, pp.421~427 (1981)
- Metz B, "Climate Change 2007: Mitigation of Climate Change" (2007), Cambridge University Press
- Murashige T, "A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures", Physiol Plant, 15, pp.473~497 (1962)
- Ostrolucka MG, "Utilization of meristem cultures in propagation of oak (Quercus sp.)", Genet Pol, 35, pp.161~169 (1994)
- 박병배, "묘포에서 질소, 인, 칼륨 비료주기가 물푸레나무, 들메나무, 잣나무, 전나무의 생장 및 양분에 미치는 영향", 한국산림과학회지, 99(1), pp.85~95 (2010), 한국산림과학회
- 박동진, "Establishment of Selection Method for Cold-Tolerant Individuals through Evaluating Tolerance of Evergreen Quercus spp. against Cold Stress", Journal of forest and environmental science, 35(4), pp.232~239 (2019), 산림과학연구소[DOI]
- Park JC, "Studies on the Growth of Seedlings and Transplanting in Quercus myrsinaefolia Blume and Q. glauca Thunb", MS, pp.2007
- Sanchez MC, "Requirements for in vitro rooting of Quercus robur and Q. rubra shoots derived from mature trees", Tree Physiol, 16, pp.673~680 (1996)
- 손석규, "종가시나무(Quercus glauca)의 육종집단 조성과 기후인자", 한국농림기상학회지, 13(3), pp.109~114 (2011), 한국농림기상학회
- 송기선, "굴거리나무 2년생 용기묘의 피음수준별 묘목품질 특성", 한국산림과학회지, 104(3), pp.390~396 (2015), 한국산림과학회[DOI]
- Stanys V, "Effect of phytohormones and stratification on morphogenesis of Paeonia lactiflora Pall. isolated embryos", Biologija, 18, pp.27~30 (2007)
- Urbanova T, "Gibberellins and seed germination", Annu Plant Rev, 49, pp.253~284 (2016)
- Van Waes JM, "In vitro germination of some Western European orchids", Physiol Plant, 67, pp.253~261 (1986)
- Vidal N, "Developmental stages during the rooting of in-vitro-cultured Quercus robur shoots from material of juvenile and mature origin", Tree Physiol, 23, pp.1247~1254 (2003)
- Vudala SM, "Seed storage and asymbiotic germination of Hadrolaelia grandis(Orchidaceae)", S Afr J Bot, 108, pp.1~7 (2017)
- Wen F, "Proteomics reveals the effects of gibberellic acid (GA3) on salt-stressed rice (Oryza sativa L.) shoots", Plant Sci, 178, pp.170~175 (2010)
- Zeiger E, "Stomatal Limitation to Carbon Gain in Paphiopedilum sp. (Orchidaceae)and its Reversal by Blue Light", Plant Physiol, 77, pp.456~460 (1985)