감귤 분자육종을 위한 분자표지 개발 현황 및 전망
Current status and prospects of molecular marker development for systematic breeding program in citrus
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
세계적인 과수작물로서의 경제적 중요성에도 불구하고, 감귤 생산은 주로 자연교잡 실생이나 눈 돌연변이로부터의선발 또는 단순 품종 도입 등을 통해 이루어지고 있는 실정이다. 긴 유년기, 다배성, 자가불화합성과 같은 감귤 고유의식물학적 특성, 주요 형질들(병저항성, 수량성, 품질 등)의QTL에 의한 조절 등은 전통 육종을 통한 우수 품종의 개발을 어렵게 하는 요인이다. 지구 온난화에 의한 생산 여건의급격한 변화, 소비자 요구 다양화 등은 고품질 감귤의 조기선발과 안정적 생산, 품종 다양화, 육종 비용 절감 등을 위한체계적인 감귤 분자육종 프로그램의 도입을 요구하고 있다. 동위효소를 이용한 최초의 감귤 연관지도 작성이 이루어진 이래, 다양한 분자표지를 이용한 연관지도 작성, 생물(CTV, CiLV, ABS, 선충] 및 비생물적(염분, 저온) 스트레스, 아포믹시스, 다배성, 과실착색(카로티노이드, 안토시아닌), 무종자, 웅성불임, 신맛 적음, 생식, 형태(나무, 잎, 꽃, 열매등), 과실 품질, 종자수, 수량성, 조기 착과 등과 연관된 분자표지 발굴, QTL 맵핑 등이 이루어졌다. CTV 저항성과 적육(안토시아닌 축적) 형질에 대해서는 유전자 클로닝이 이루어졌고, 교배 육종 효율 증대 및 비용 절감을 위해 교잡배와주심배를 구분하기 위한 다수의 simple sequence repeat (SSR) 분자표지가 개발되었다. 최근, 스위트오렌지와 ‘클레멘타인’ 만다린에 대한 고품질의 표준 유전체가 완성되어 유전체 기반 감귤 분자육종을 위한 토대가 마련되었다. 표준 유전체 정보를 토대로 대규모 분자표지(SNP, SSR, InDel) 기반의 표준 연관 및 물리지도 작성, 비교 유전체 지도 작성, gene annotation, 전사체 분석 등이 활발히 이루어지고 있다. 감귤유전자원 및 핵심집단에 대해 표준 유전체 기반 비교 유전체 분석, GBS (genotyping-by-sequencing), GWAS (genome wide association study) 등을 통해 감귤의 다양한 형질과 연관된 분자마커 발굴 및 개발, 유용/변이 유전자 클로닝 등에 관한 연구가 가속화될 것으로 전망된다. 또한 표적 유전체 교정 및 VIGS (virus-induced gene silencing) 기술도 유전자 마커의 검증을 비롯한 감귤 분자육종 프로그램에 활발히 이용될 것이다.
Abstract
Citrus is an economically important fruit crop widely growing worldwide. However, citrus production largely depends on natural hybrid selection and bud sport mutation. Unique botanical features including long juvenility, polyembryony, and QTL that controls major agronomic traits can hinder the development of superior variety by conventional breeding. Diverse factors including drastic changes of citrus production environment due to global warming and changes in market trends require systematic molecular breeding program for early selection of elite candidates with target traits, sustainable production of high quality fruits, cultivar diversification, and cost-effective breeding. Since the construction of the first genetic linkage map using isozymes, citrus scientists have constructed linkage maps using various DNA-based markers and developed molecular markers related to biotic and abiotic stresses, polyembryony, fruit coloration, seedlessness, male sterility, acidless, morphology, fruit quality, seed number, yield, early fruit setting traits, and QTL mapping on genetic maps. Genes closely related to CTV resistance and flesh color have been cloned. SSR markers for identifying zygotic and nucellar individuals will contribute to cost-effective breeding. The two high quality citrus reference genomes recently released are being efficiently used for genomics-based molecular breeding such as construction of reference linkage/ physical maps and comparative genome mapping. In the near future, the development of DNA molecular markers tightly linked to various agronomic traits and the cloning of useful and/or variant genes will be accelerated through comparative genome analysis using citrus core collection and genome-wide approaches such as genotyping-by-sequencing and genome wide association study.
참고문헌 (99)
- Akimitsu K, "Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus", Mol Plant Pathol, 4, pp.435~446 (2003)
- Altaf S, "Morphogenetic characterization of seeded and seedless varieties of Kinnow Mandarin (Citrus reticulata Blanco)", Australian J Crop Sci, 8, pp.1542~1549 (2014)
- Asíns MJ, "The position of the major QTL for citrus tristeza virus resistance is conserved among Citrus grandis, C. aurantium and Poncirus trifoliata", Mol Breed, 29, pp.575~587 (2012)
- Asíns MJ, "Genetic analysis of reproductive, vegetative and fruit quality traits to improve Citrus varieties", Tree Genet Genomes, 11, pp.117~ (2015)
- Balal RM, "Comparative studies on the physiobiochemical, enzymatic, and ionic modifications in salt-tolerant and salt-sensitive citrus rootstocks under NaCl stress", J Amer Soc Hort Sci, 137, pp.86~95 (2012)
- Barcaccia G, "Apomixis in plant reproduction: a novel perspective on an old dilemma", Plant Reprod, 26, pp.159~179 (2013)
- Bastianel M, "Identification of zygotic and nucellar tangerine seedlings (Citrus spp.) using RAPD", Genet Mol Biol, 21, pp.123~127 (1998)
- Bastianel M, "Inheritance and heritability of resistance to citrus leprosis", Phytopathol, 96, pp.1092~1096 (2006)
- Bastianel M, "Quantitative trait loci analysis of citrus leprosis resistance in an interspecific backcross family of (Citrus reticulata Blanco x C. sinensis L. Osbeck) x C. sinensis L. Osb", Euphytica, 169, pp.101~111 (2009)
- Biswas MK, "Genome wide characterization of short tandem repeat markers in sweet orange (Citrus sinensis)", PLoS One, 9, pp.e104182~ (2014)
- Bonina FP, "Evaluation of oxidative stress in diabetic patients after supplementation with a standardised red orange extract", Diabetes Nutr Metab, 15, pp.14~19 (2002)
- Butelli E, "Retrotransposons control fruit-specific, cold-dependent accumulation of anthocyanins in blood oranges", Plant Cell, 24, pp.1242~1255 (2012)
- Cai Q, "Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD)markers and RFLP mapping of cold-acclimation-responsive loci", Theor Appl Genet, 89, pp.606~614 (1994)
- Cameron JW, "Acidity and total soluble solids in citrus hybrids and advanced crosses involving acidless orange and acidless pummelo", J Am Soc Hortic Sci, 102, pp.198~201 (1977)
- Cameron JW, "Sexual and nucellar embryony in F1 hybrids and advanced crosses of Citrus and Poncirus", J Am Soc Hort Sci, 104, pp.408~410 (1979)
- citrate uptake by tonoplast vesicles of acidless citrus juice cells, "J Am Soc Hortic Sci", 1995, 510~514
- 채치원, "Development of a SCAR Marker Linked to Male Fertility Traits in ‘Jinkyool’ (Citrus sunki)", 생명과학회지, 21(12), pp.1659~1665 (2011), 한국생명과학회
- Chavez DJ, "Identification of markers linked to seedlessness in Citrus kinokuni hort. ex Tanaka and its progeny using bulked segregant analysis", HortSci, 46, pp.693~697 (2011)
- Chen C, "Pigments in Fruits and Vegetables", pp.165~187 (2015), Springer
- Cuenca J, "Genetically based location from triploid populations and gene ontology of a 3.3-mb genome region linked to Alternaria brown spot resistance in citrus reveal clusters of resistance genes", PLoS One, 8, pp.e76755~ (2013)
- Dalkilic Z, "Linkage of an Alternaria disease resistance gene in mandarin hybrids with RAPD fragments", J Am Soc Hort Sci, 130, pp.191~195 (2005)
- Davies FS, "Citrus" (1994), CAB International
- De Oliveira AC, "Identification of citrus hybrids through the combination of leaf apex morphology and SSR markers", Euphytica, 128, pp.397~403 (2002)
- De Pascual-Teresa, S, "Flavanols and anthocyanins in cardiovascular health: A review of current evidence", Int J Mol Sci, 11, pp.1679~1703 (2010)
- Deng Z, "Development and characterization of SCAR markers linked to the citrus tristeza virus resistance gene from Poncirus trifoliata", Genome, 40, pp.697~704 (1997)
- Deng Z, "Cloning and characterization of NBS-LRR class resistance-gene candidate sequences in citrus", Theor Appl Genet, 101, pp.814~822 (2000)
- Deng Z, "Construction of a bacterial artificial chromosome (BAC) library for citrus and identification of BAC contigs containing resistance gene candidates", Theor Appl Genet, 102, pp.1177~1184 (2001)
- Deng Z, "Fine genetic mapping and BAC contig development for the citrus tristeza virus resistance gene locus in Poncirus trifoliata (Raf.)", Mol Genet Genomics, 265, pp.739~747 (2001)
- Donmez D, "Genetic transformation in Citrus", Scientific World J, 2013, pp.491207~ (2013)
- Fang DQ, "A high-resolution linkage map of the citrus tristeza virus resistance gene region in Poncirus trifoliata (L.) Raf", Genetics, 150, pp.883~890 (1998)
- Fang DQ, "Development of molecular markers linked to a gene controlling fruit acidity in citrus", Genome, 40, pp.841~849 (1997)
- Fang DQ, "A novel gene conferring citrus tristeza virus resistance in Citrus maxima (Burm.) Merrill", Hortsci, 34, pp.334~335 (1999)
- Gaj T, "ZFN, TALEN, and CRISPR", Casbased methods for genome engineering, 2013, pp.31, 397~405
- García R, "Genetic analysis of apomixis in Citrus and Poncirus by molecular markers", Theor Appl Genet, 99, pp.511~518 (1999)
- García MR, "QTL analysis of yield and seed number in Citrus", Theor Appl Genet, 101, pp.487~493 (2000)
- Garnsey SM, "Identification of citrus tristeza virus resistance in citrus relatives and its potential applications", Phytophylactica, 19, pp.187~191 (1987)
- Garnsey SM, "Differential susceptibility of pummelo and Swingle citrumelo to isolates of citrus tristeza virus", Proc. 13th Conf. Intl. Organ. Citrus Virologists, pp.138~146 (1997), Univ. California Press
- Gmitter FG Jr, "A localized linkage map of the citrus tristeza virus resistance gene region", Theor Appl Genet, 92, pp.688~695 (1996)
- Gmitter FG Jr, "Citrus Genetics, Breeding and Biotechnology", pp.287~305 (2007), CAB International
- Gulsen O, "A new citrus linkage map based on SRAP, SSR, ISSR, POGP, RGA and RAPD markers", Euphytica, 173, pp.265~277 (2010)
- Gulsen O, "QTL analysis and regression model for estimating fruit setting in young citrus trees based on molecular markers", Sci Hortic, 130, pp.418~424 (2011)
- Sang Heon Han, "Expression of Green Fluorescent Protein Gene in the Callus of Satsuma Mandarin (Citrus unshiu cv. Miyagawa Wase) by Agrobacterium-Mediated Transformation", Horticulture, Environment, and Biotechnology, 46(1), pp.39~42 (2005), 한국원예학회
- Hong QB, "Two complementary dominant genes controlling apomixis in genus Citrus and Poncirus", Acta Genet Sin, 28, pp.1062~1067 (2001)
- Hong QB, "QTL mapping of citrus freeze tolerance", Acta Hort (2015)[DOI]
- Iwamasa M, "Inheritance of nucellar embryony in Citrus", Bull Hort Res Sta Japan, ser B, 7, pp.1~10 (1967)
- Jayaprakasha GK, "In vitro evaluation of the antioxidant activities in fruit extracts from citron and blood orange", Food Chem, 101, pp.410~418 (2007)
- Jia H, "Targeted genome editing of sweet orange using Cas9", sgRNA, 2014, pp.9, e93806~
- 강성구, "감귤의 다배성 연관 SCAR 마커 개발", 원예과학기술지, 26(1), pp.51~55 (2008), 한국원예학회
- Kepiro JL, "Citrus Genetics, Breeding and Biotechnology", pp.141~149 (2007), CAB International
- Kepiro JL, "AFLP markers closely linked to a major gene essential for nucellar embryony (apomixis) in Citrus maxima × Poncirus trifoliata", Tree Genet Genomes, 6, pp.1~11 (2010)
- 김호방, "감귤 유전체 연구 동향 및 전망", Journal of Plant Biotechnology, 42(4), pp.326~335 (2015), 한국식물생명공학회[DOI]
- Kim JH, "Segregation of self-incompatible hybrid seedlings in crosses with grapefruit and possible RAPD markers for the S gene alleles", J Faculty Agric Kyushu Univ, 58, pp.269~275 (2013)
- Kohmoto K, "Correlation of resistance and susceptibility of citrus to Alternaria alternata with sensitivity to host-specific toxins", Phytopathol, 81, pp.719~722 (1991)
- Koltunow AM, "Polyembryony in Citrus: Accumulation of seed storage proteins in seeds and in embryos cultured in vitro", Plant Physiol, 110, pp.599~609 (1996)
- Ladaniya M, "Citrus fruit: biology, technology and evaluation" (2008), Elsevier Inc
- Latado RR, "Anthocyanin accumulation and physical and chemical characteristics of blood orange fruits during cold storage", Rev Bras Frutic, 30, pp.604~610 (2008)
- Ling P, "Inheritance of citrus nematode resistance and its linkage with molecular markers", Theor Appl Genet, 100, pp.1010~1017 (2000)
- Maas EV, "Salinity and citriculture", Tree Physiol, 12, pp.195~216 (1993)
- Mestre PF, "Efficient search for new resistant genotypes to the citrus tristeza closterovirus in the orange subfamily Aurantioideae", Theor Appl Genet, 95, pp.1282~1288 (1997)
- Mestre PF, "Molecular markers flanking citrus tristeza virus resistance gene from Poncirus trifoliata (L) Raf", Theor Appl Genet, 94, pp.458~464 (1997)
- Nakano M, "Segregation of plants with undeveloped anthers among hybrids derived from the seed parent, ‘Kiyomi’ (Citrus unshiu × C. sinensis)", J Japan Soc Hort Sci, 70, pp.539~545 (2001)
- Nakano M, "Mapping and haplotyping of the flanking region of the polyembryony locus in Citrus unshiu Marcow", J Japanese Soc Hort Sci, 77, pp.109~114 (2008)
- Nakano M, "Characterization of genes associated with polyembryony and in vitro somatic embryogenesis in Citrus", Tree Genet Genomes, 9, pp.795~803 (2013)
- Nicolosi E, "Citrus Genetics, Breeding and Biotechnology", pp.19~43 (2007), CAB International
- Ollitrault P, "Citrus Genetics, Breeding and Biotechnology", pp.197~218 (2007), CAB International
- Ollitrault P, "A reference genetic map of Citrus clementina hort. ex Tan.; citrus evolution inferences from comparative mapping", BMC Genomics, 13, pp.593~ (2012)
- Parlevliet JE, "Evidence on the inheritance of nucellar embryony in Citrus", Proc Am Soc Hortic Sci, 74, pp.252~260 (1959)
- Penjor T, "Phylogenetic relationships of Citrus and its relatives based on matK gene sequences", PLoS ONE, 8(4), pp.e62574~ (2013)
- Pieringer AP, "Identification of nucellar and zygotic citrus seedlings by infrared spectroscopy", J Am Soc Hort Sci, 86, pp.226~234 (1967)
- Panha Pok, "Characterization of Microspore Development and Pollen Tube Growth Response to Self- and Cross-pollination in Jeju Old Local Citrus Species", Horticulture, Environment, and Biotechnology, 56(2), pp.225~232 (2015), 한국원예학회[DOI]
- Raga V, "Segregation and linkage analyses in two complex populations derived from the citrus rootstock Cleopatra mandarin. Inheritance of seed reproductive traits", Tree Genet Genomes, 8, pp.1061~1071 (2012)
- Raga V, "Inheritance of rootstock effects and their association with salt-tolerance candidate genes in a progeny derived from ‘Volkamer’ lemon", J Amer Soc Hort Sci, 139, pp.518~528 (2014)
- Raga V, "Genetic analysis of salt tolerance in a progeny derived from the citrus rootstocks Cleopatra mandarin and trifoliate orange", Tree Genet Genomes, 12, pp.34~ (2016)
- Rai M, "Refinement of the Citrus tristeza virus resistance gene (Ctv) positional map in Poncirus trifoliata and generation of transgenic grapefruit (Citrus paradisi) plant lines with candidate resistance genes in this region", Plant Mol Biol, 61, pp.399~414 (2006)
- Rao MN, "Characterization of zygotic and nucellar seedlings from sour orange-like Citrus rootstock candidates using RAPD and EST-SSR markers", Tree Genet Genomes, 4, pp.113~124 (2008)
- Riso P, "Effects of blood orange juice intake on antioxidant bioavailability and on different markers related to oxidative stress", J Agric Food Chem, 53, pp.941~947 (2005)
- Rodrigues JCV, "Citrus leprosis virus vectored by Brevipalpus phoenicis (Acari:Tenuipalpidae) on citrus in Brazil", Exp Appl Acarol, 30, pp.161~179 (2003)
- Ruiz C, "A quick methodology to identify sexual seedlings in citrus breeding programs using SSR markers", Euphytica, 112, pp.89~94 (2000)
- Mehtap S¸ ahin-C¸ evik, "Quantitative trait loci analysis of morphological traits in Citrus", Plant Biotechnology Reports, 6(1), pp.47~57 (2012), 한국식물생명공학회[DOI]
- Soost RK, "Identification of nucellar and zygotic seedlings of Citrus with leaf isozymes", HortSci, 15, pp.728~729 (1980)
- Talon M, "Citrus genomics", Inter J Plant Genomics (2008)
- Titta L, "Blood orange juice inhibits fat accumulation in mice", Int J Obes (Lond), 34, pp.578~588 (2010)
- Tozlu I, "QTL analysis of Na +and Cl -accumulation related traits in an intergeneric BC1 progeny of Citrus and Poncirus under saline and nonsaline environments", Genome, 42, pp.692~705 (1999)
- Tozlu I, "QTL analysis of morphological traits in an intergeneric BC1 progeny of Citrus and Poncirus under saline and non-saline environments", Genome, 42, pp.1020~1029 (1999)
- Ueno I, "Embryo number of various varieties of Citrus and its relatives", Bull Hort Res Sta Japan ser. B, 7, pp.11~22 (1967)
- Weber CA, "Mapping freeze tolerance quantitative trait loci in a Citrus grandis x Poncirus trifoliata F-1 pseudo-testcross using molecular markers", J Amer Soc Hort Sci, 128, pp.508~514 (2003)
- Weinbaum SA, "Rapid screening of ‘Satsuma’ mandarin progeny to distinguish nucellar and zygotic seedlings", Hort Sci, 17, pp.239~240 (1982)
- Woo JW, "DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins", Nat Biotechnol, 33, pp.1162~1164 (2015)
- Wu GA, "Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication", Nat Biotechnol, 32, pp.656~662 (2014)
- Xiao JP, "Identification of AFLP fragments linked to seedlessness in Ponkan mandarin (Citrus reticulata Blanco) and conversion to SCAR markers", Sci Hortic, 121, pp.505~510 (2009)
- Xu Q, "The draft genome of sweet orange (Citrus sinensis)", Nat Genet, 45, pp.59~66 (2013)
- Yamasaki A, "Histological study of expression of seedlessness in Citrus kinokuni ‘Mukaku Kishu’ and its progenies", J Amer Soc Hort Sci, 132, pp.869~875 (2007)
- Yang ZN, "Construction of a 1.2-Mb contig including the citrus tristeza virus resistance gene locus using a bacterial artificial chromosome library of Poncirus trifoliata (L.) Raf", Genome, 44, pp.382~393 (2001)
- Yang ZN, "Sequence analysis of a 282-kb region surrounding the citrus tristeza virus resistance gene (Ctv) locus in Poncirus trifoliata L. Raf", Plant Physiol, 131, pp.482~492 (2003)
- Yildiz E, "Identification of zygotic and nucellar individuals produced from several citrus crosses using SSRs markers", Not Bot Horti Agrobo, 41, pp.478~484 (2013)
- Yoshida T, "Inheritance of susceptibility to citrus tristeza virus in trifoliate orange", Bull Fruit Trees Res Sta, 82, pp.17~26 (1985)
- Yoshida T, "Inheritance of immunity to citrus tristeza virus of trifoliate orange in some citrus intergeneric hybrids", Bull Fruit Trees Res Sta, 90, pp.33~43 (1993)
- Jin-Ung Yun, "Identification of Zygotic and Nucellar Mandarin Seedlings Using Randomly Amplified Polymorphic DNA", Horticulture, Environment, and Biotechnology, 48(3), pp.171~175 (2007), 한국원예학회
- Zeng W, "Microsatellite polymorphism is likely involved in phytoene synthase activity in Citrus", Plant Cell Tiss Organ Cult, 113, pp.449~458 (2013)
