전사인자 OsNAC58 과발현을 통한 벼 흰잎마름병 저항성 증진 벼
Overexpression of rice NAC transcription factor OsNAC58 on increased resistance to bacterial leaf blight
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Bacterial blight in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) greatly reduces the growth and productivity of this important food crop. Therefore, we sought to increase the resistance of rice to bacterial blight by using a NAC (NAM, ATAF, and CUC) transcription factor, one of the plant-specific transcription factors that is known to be involved in biotic/abiotic stress resistance. By isolating the OsNAC58 gene from rice and analyzing its biological functions related to Xoo resistance, phylogenetic analysis showed that OsNAC58 belongs to group III. To investigate the biological relationship between bacterial leaf blight (BLB) and OsNAC58 in rice, we constructed a vector for overexpression in rice and generated transgenic rice. The expression analysis resulting from use of RT-PCR showed that OsNAC58- overexpressed transgenic rice exhibited higher levels of OsNAC58 expression than wild types. Further, subcellular localization analysis using rice protoplasts showed that the 35S/OsNAC58-SmGFP fusion protein was localized in the nuclei. Thirteen OsNAC58-overexpressed transgenic rice lines, with high expression levels of OsNAC58, showed more resistant to Xoo than did the wild types. Together, these results suggest that the OsNAC58 gene of rice regulates the rice disease resistance mechanism in the nucleus upon invasion of the rice bacterial blight pathogen Xoo.
Abstract
Bacterial blight in rice caused by Xanthomonas oryzae pv. oryzae (Xoo) greatly reduces the growth and productivity of this important food crop. Therefore, we sought to increase the resistance of rice to bacterial blight by using a NAC (NAM, ATAF, and CUC) transcription factor, one of the plant-specific transcription factors that is known to be involved in biotic/abiotic stress resistance. By isolating the OsNAC58 gene from rice and analyzing its biological functions related to Xoo resistance, phylogenetic analysis showed that OsNAC58 belongs to group III. To investigate the biological relationship between bacterial leaf blight (BLB) and OsNAC58 in rice, we constructed a vector for overexpression in rice and generated transgenic rice. The expression analysis resulting from use of RT-PCR showed that OsNAC58- overexpressed transgenic rice exhibited higher levels of OsNAC58 expression than wild types. Further, subcellular localization analysis using rice protoplasts showed that the 35S/OsNAC58-SmGFP fusion protein was localized in the nuclei. Thirteen OsNAC58-overexpressed transgenic rice lines, with high expression levels of OsNAC58, showed more resistant to Xoo than did the wild types. Together, these results suggest that the OsNAC58 gene of rice regulates the rice disease resistance mechanism in the nucleus upon invasion of the rice bacterial blight pathogen Xoo.
참고문헌 (37)
- Aida M, "Genes involved in organ separation in Arabidopsis: an analysis of the cup-shaped cotyledon mutant", Plant Cell, 9, pp.841~857 (1997)
- Dai LY, "Recent advances in cloning and characterization of disease resistance genes in rice", J Integrative Plant Biol, 49(1), pp.112~119 (2007)
- Earley KW, "Gateway-compatible vectors for plant functional genomics and proteomics", Plant J, 45(4), pp.616~629 (2006)
- Ernst HA, "Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors", EMBO Rep, 5, pp.297~303 (2004)
- Fang Y, "Systematic sequence analysis and identification of tissue-specific or stress-responsive genes of NAC transcription factor family in rice", Mol Gen Genet, 280(6), pp.547~563 (2008)
- Fang Y, "Conserved miR164-targeted NAC genes negatively regulate drought resistance in rice", J Exp Bot, 65(8), pp.2119~2135 (2014)
- Hiei Y, "Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA", Plant J, 6(2), pp.271~282 (1994)
- Hu H, "Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice", Proc Natl Acad Sci USA, 103, pp.12987~12992 (2006)
- Hu R, "Comprehensive analysis of NAC domain transcription factor gene family in Populus trichocarpa", BMC Plant Biol, 10, pp.145~ (2010)
- Huang L, "Rice NAC transcription factor ONAC095 plays opposite roles in drought and cold stress tolerance", BMC Plant Biol, 16(1), pp.203~ (2016)
- Hussain SS, "Transcription factors as tools to engineer enhanced drought stress tolerance in plants", Biotechnology Progress, 27, pp.297~306 (2011)
- Jiang J, "High efficiency tansformation of U.S. rice lines from mature seed-derived calli and segregation of glufosinate resistance under field conditions", Crop Sci, 40, pp.729~1741 (2000)
- Kaneda T, "The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death", EMBO J, 28, pp.926~936 (2009)
- Kauffman HE, "An improved technique for evaluation of resistance of rice varieties to Xanthomonas oryzae", Plant Disease Reporter, 57, pp.537~541 (1973)
- Karganilla A, "A comparative study of culture media for Xanthomonas oryzae", Philipp Agric, 57, pp.141~152 (1973)
- Kim HS, "Identification of a calmodulin-binding NAC protein as a transcriptional repressor in Arabidopsis", J Biol Chem, 282(50), pp.36292~36302 (2007)
- Kleinow T, "NAC domain transcription factor ATAF1 interacts with SNF1-related kinases and silencing of its subfamily causes severe developmental defects in Arabidopsis", Plant Science, 177, pp.360~370 (2009)
- Le DT, "Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress", DNA Res, 18, pp.263~276 (2011)
- Lee D-K, "The rice OsNAC6transcription factor orchestrates multiple molecular mechanisms involving root structural adaptions and nicotianamine biosynthesis for drought tolerance", Plant Biotechnology Journal, 15, pp.754~764 (2017)
- Li ZK, "Complex genetic networks underlying the defensive system of rice (Oryza sativa L.) to Xanthomonas oryzae pv. Oryzae", Proc Nat Acad Sci USA, 103(21), pp.7994~7999 (2006)
- Lin R, "Rice gene OsNAC19 encodes a novel NAC-domain transcription factor and responds to infection by Magnaporthe grisea", Plant Science, 172, pp.120~130 (2007)
- Mundt CC, "Variation for aggressiveness within and between lineages of Xanthomonas oryzae pv. oryzae", Plant Pathology, 51, pp.163~168 (2002)
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol. Planta, 15, pp.473~497 (1962)
- Nakashima K, "Functional analysis of a NAC-type transcription factor OsNAC6 involved in abiotic and biotic stress-responsive gene expression in rice", Plant J, 51(4), pp.617~630 (2007)
- Nuruzzaman M, "Genome-wide analysis of NAC transcription factor family in rice", Gene, 465, pp.30~44 (2010)
- Nuruzzaman M, "Roles of NAC transcription factors in the regulation of biotic and abiotic stress responses in plants", Front Microbiol, 4, pp.248~ (2013)
- Nuruzzaman M, "NAC transcription factor family genes are differentially expressed in rice during infections with Rice dwarf virus, Rice black-streaked dwarf virus, Rice grassy stunt virus, Rice ragged stunt virus, and Rice transitory yellowing virus", Front Plant Sci, 6, pp.676~ (2015)
- 박상렬, "전사인자 OsNAC69-과발현을 통한 흰잎마름병 저항성 벼 제작", 한국육종학회지, 43(5), pp.457~463 (2011), 한국육종학회
- Puranik S, "NAC proteins: regulation and role in stress tolerance", Trends Plant Sci, 17, pp.369~381 (2012)
- Sambrook J, "Molecular Cloning: A Laboratory Manual" (1989), Cold Spring Harbor Laboratory
- Shen J, "The NAC-type transcription factor OsNAC2 regulates ABAdependent genes and abiotic stress tolerance in rice", Scientific Reports, 7, pp.40641~ (2017)
- 신문식, "벼흰잎마름병 저항성 품종 육성 및 금후 연구 방향", 한국육종학회지, 43(4), pp.241~251 (2011), 한국육종학회
- Sperotto R, "Identification of up-regulated genes in flag leaves during rice grain filling and characterization of OsNAC5, a new ABA-dependent transcription factor", Planta, 230, pp.985~1002 (2009)
- Sun L, "Comprehensive analysis suggests overlapping expression of rice ONAC transcription factors in abiotic and biotic stress responses", Int J Mol Sci, 16, pp.4306~4326 (2015)
- Yokotani N, "OsNAC111, a blast disease-responsive transcription factor in rice, positively regulates the expression of defense-related genes", Mol Plant Microbe Interact, 27(10), pp.1027~1034 (2014)
- Yoshii M, "Disruption of a novel gene for a NAC-domain protein in rice confers resistance to Rice dwarf virus", Plant J, 57(4), pp.615~625 (2009)
- Zhang Y, "A highly efficient rice green tissue protoplast system for transient gene expression and studying light", chloroplast-related processes, 2011, pp.7(1), 30~
