RAPD와 SRAP 마커를 이용한 참다래 유전자원의 유전적 다양성
Genetic diversity in kiwifruit germplasm evaluated using RAPD and SRAP markers
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
In this study, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were used for evaluation of genetic diversity of 61 kiwifruit (Actinidia spp.) germplasms including domestic and overseas collection cultivars. Forty RAPD primers were detected in a total of 230 polymorphic bands with an average of 5.75. Thirty-two SRAP primer combinations were detected in a total of 204 polymorphic bands with an average 6.38. By unweighted pair-group method arithmetic average cluster analysis using 434 polymorphic bands, kiwifruit germplasms were classified in three groups with similarity value of 0.680. Cluster I consisted of 46 kiwifruit germplasms belonging to A. deliciosa, A. chinensis, A. deliciosa × A. arguta, A. chinensis × A. arguta, and A. chinensis × A. deliciosa. Cluster II consisted of seven germplasms belonging to A. arguta and ‘Skinny Green’, a cultivar derived from a cross between A. arguta and A. deliciosa. Cluster III consisted of seven germplasms belonging to A. rufa, A. hemsleyana, A. macrosperma, A. polygama, and A. eriantha. Genetic similarity values among tested kiwifruit germplasms ranged from 0.479-0.991, and average similarity value was 0.717. Similarity value was highest (0.991) between NHK0038 (A. deliciosa) and NHK0040 (A. deliciosa), and lowest (0.479) between ‘Hayward’ (A. deliciosa) and K5-1-22 (A. arguta).
Abstract
In this study, random amplified polymorphic DNA (RAPD) and sequence-related amplified polymorphism (SRAP) analyses were used for evaluation of genetic diversity of 61 kiwifruit (Actinidia spp.) germplasms including domestic and overseas collection cultivars. Forty RAPD primers were detected in a total of 230 polymorphic bands with an average of 5.75. Thirty-two SRAP primer combinations were detected in a total of 204 polymorphic bands with an average 6.38. By unweighted pair-group method arithmetic average cluster analysis using 434 polymorphic bands, kiwifruit germplasms were classified in three groups with similarity value of 0.680. Cluster I consisted of 46 kiwifruit germplasms belonging to A. deliciosa, A. chinensis, A. deliciosa × A. arguta, A. chinensis × A. arguta, and A. chinensis × A. deliciosa. Cluster II consisted of seven germplasms belonging to A. arguta and ‘Skinny Green’, a cultivar derived from a cross between A. arguta and A. deliciosa. Cluster III consisted of seven germplasms belonging to A. rufa, A. hemsleyana, A. macrosperma, A. polygama, and A. eriantha. Genetic similarity values among tested kiwifruit germplasms ranged from 0.479-0.991, and average similarity value was 0.717. Similarity value was highest (0.991) between NHK0038 (A. deliciosa) and NHK0040 (A. deliciosa), and lowest (0.479) between ‘Hayward’ (A. deliciosa) and K5-1-22 (A. arguta).
참고문헌 (26)
- Ahmad R, "Genotyping of peach and nectarine cultivars with SSR and SRAP molecular markers", J Am Soc Hort Sci, 129, pp.204~210 (2004)
- Budak H, "Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs", Theor Appl Genet, 109, pp.280~288 (2004)
- 조강희, "RAPD와 SSR 마커를 이용한 사과 품종의 유전적 다양성 분석", 한국육종학회지, 42(5), pp.525~533 (2010), 한국육종학회
- Cipriani G, "Screening RAPD primers for molecular taxonomy and cultivar fingerprinting in the genus Actinidia", Euphytica, 90, pp.169~174 (1996)
- Crowhurst RN, "Restriction-fragment-length-polymorphisms in the genus Actinidia (Actinidiaceae)", Plant Syst Evol, 172, pp.193~203 (1990)
- Ellsworth DL, "Artifactual variation in randomly amplified polymorphic DNA banding patterns", Biotechniques, 14, pp.214~217 (1993)
- Ferguson AR, "The need for characterisation and evaluation of germplasms: kiwifruit as an example", Euphytica, 154, pp.371~382 (2007)
- Ferguson AR, "Genetic resources of kiwifruit:domestication and breeding", Hor Rev, 33, pp.1~121 (2007)
- Ferguson AR, "Fruit breeding, Vol. II: Vine and small fruit crops", pp.371~417 (1996), Prentice Hall
- Guo D, "Genetic variability and relationships between and within grape cultivated varieties and wild species based on SRAP markers", Tree Genet Genom, 8, pp.789~800 (2012)
- Huang HW, "Phylogenetic relationships in Actinidia as revealed by RAPD analysis", J Amer Soc Hort Sci, 127, pp.759~766 (2002)
- Jaeger SR, "Consumer evaluation of novel kiwifruit: willingness-to-pay", J Sci Food Agric, 85, pp.2519~2526 (2005)
- Kabaluk JT, "Actinidia argutacharacteristics relevant to commercialization production", Fruit Var J, 51, pp.117~122 (1997)
- Korkovelos AE, "Effectiveness of SSR molecular markers in evaluating the phylogenetic relationships among eight Actinidia species", Sci Hortic, 116, pp.305~310 (2008)
- Li G, "Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction:its application to mapping and gene tagging in Brassica", Theor Appl Genet, 103, pp.455~461 (2001)
- Liu Y, "Genetic variation and natural hybridization among sympatric Actinidia species and the implications for introgression breeding of kiwifruit", Tree Genet Genomes, 6, pp.801~813 (2010)
- Nishiyama I, "Fruits of the Actinidia genus", Adv Food Nutr Res, 52, pp.293~324 (2007)
- Novo M, "Identification and sequence characterisation of molecular markers polymorphic between male kiwifruit (Actinidia chinensis var. deliciosa (A. Chev.) A. Chev.) accessions exhibiting different flowering time", Euphytica, 175, pp.109~121 (2010)
- Palombi MA, "Comparison between RAPD and SSR molecular markers in detecting genetic variation in kiwifruit (Actinidia deliciosa A. Chev)", Plant Cell Rep, 20, pp.1061~1066 (2002)
- 박영기, "다래(Actinidia arguta) 및 교잡종 다래(A. arguta × A. deliciosa)의 수관층위에 따른 광합성, 엽 그리고 과실의 특성 변화", 한국산림과학회지, 100(1), pp.8~13 (2011), 한국산림과학회
- Prado MJ, "Adventitious plant regeneration on leaf explants from adult male kiwifruit and AFLP analysis of genetic variation", Plant Cell Tiss Cult, 88, pp.1~10 (2007)
- Stãnicã F, "New selections of Actinidia arguta from the Romanian breeding program", Acta Hortic, 753, pp.263~267 (2007)
- Uzun A, "SRAP based genetic analysis of some apricot cultivars", Romanian Biotechnol Lett, 15, pp.5396~5404 (2010)
- Williams MH, "Development and commercialization of ‘Baby Kiwi’ (Actinidia argute Planch)", Acta Hortic, 610, pp.81~86 (2003)
- Zhao Y, "Genetic linkage maps of pear based on SRAP markers", Pak J Bot, 45, pp.1265~1271 (2013)
- Zhen Y, "Molecular characterization of kiwifruit (Actinidia) cultivars and selections using SSR markers", J Amer Soc Hort Sci, 129, pp.374~382 (2004)
