Effect of salicylic acid and yeast extract on curcuminoids biosynthesis gene expression and curcumin accumulation in cells of Curcuma zedoaria
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The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.
Abstract
The aim of this study is to evaluate the effect of yeast extract (YE) and salicylic acid (SA) on the expression of curcuminoid-biosynthesis genes (CzDCS and CURS1-3), and accumulation of curcumin in Curcuma zedoaria cell cultures. The results showed that, in cells treated with YE or SA, the expression levels of curcuminoid genes were 1.14- to 3.64-fold higher than the control (untreated cells), in which the YE exhibited a stronger effect in comparison with SA. Curcumin accumulation also tended to be similar to gene expression, curcumin contents in YE- or SA-treated cells were 1.61- to 2.53-fold higher than the control. The SA treatment at the fifth day of culture stimulated the curcumin accumulation and expression in all four genes compared to that at the beginning. While the YE treatments gave different results, the CzCURS1 and CzCURS3 genes were expressed strongly in cells that were treated at the beginning. However, the CzDCS and CzCURS2 genes showed the opposite expression pattern, they were activated strongly in the treatments at day five of the culture. However, the content of curcumin reached its maximum value on the fifth day of culture in all investigations.
참고문헌 (31)
- Abraham F, "Effect of yeast extract and chitosan on shoot proliferation, morphology and antioxidant activity of Curcuma mangga in vitro plantlets", African Journal of Biotechnology, 10, pp.7787~7795 (2011)
- Amalraj A, "Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives - A review", J Tradit Complement Med, 7, pp.205~233 (2017)
- Azam MG, "Evaluation of anti-diarrhoeal activity of Curcuma zedoaria rhizome", J Pharmacogn Phytochem, 6, pp.171~173 (2017)
- Badrhadad A, "Increasing of alphatocopherol in cell suspension cultures Elaeagnus angustifolia L", Intl J Agri Crop Sci, 5, pp.1328~1331 (2013)
- Bastianelli F, "Salicylic acid differentially affects suspension cell cultures of Lotus japonicus and one of its non-symbiotic mutants", Plant Mol Biol, 72, pp.469~483 (2010)
- Behar N, "Semi-quantitative expression studies of genes involved in biosynthesis of curcuminoid in Curcuma caesia Roxb", Indian J Biotechnol, 15, pp.491~494 (2016)
- Brand S, "A type III polyketide synthase from Wachendorfia thyrsiflora and its role in diarylheptanoid and phenylphenalenone biosynthesis", Planta, 224, pp.413~428 (2006)
- Chujo T, "Involvement of the elicitor-induced gene OsWRKY53 in the expression of defense-related genes in rice", Biochim Biophys Acta, 1769, pp.497~505 (2007)
- Dewanjee S, "Signal transducer and oxidative stress mediated modulation of phenylpropanoid pathway to enhance rosmarinic acid biosynthesis in fungi elicited whole plant culture of Solenostemon scutellarioides", Enzyme Microb Technol, 66, pp.1~9 (2014)
- Ellard-Ivey M, "Role of jasmonates in the elicitorand wound-inducible expression of defense genes in parsley and transgenic tobacco", Plant Physiol, 112, pp.183~192 (1996)
- Gilani SA, "Molecular genetic diversity of curcuminoid genes in Curcuma amada: Curcuminoid variation, consideration on species boundary and polyploidy", Biochem Syst Ecol, 61, pp.186~195 (2015)
- Hayakawa H, "Difference of curcumin content in Curcuma longa L. (Zingiberaceae) caused by hybridization with other Curcuma species", Am J Plant Sci, 2, pp.111~119 (2011)
- 정귀택, "Enhancement of Growth and Secondary Metabolite Biosynthesis: Effect of Elicitors Derived from Plants and Insects", Biotechnology and Bioprocess Engineering, 10(1), pp.73~77 (2005), 한국생물공학회
- Katsuyama Y, "Identification and characterization of multiple curcumin synthases from the herb Curcuma longa", FEBS Lett, 583, pp.2799~2803 (2009)
- Katsuyama Y, "Curcuminoid biosynthesis by two type III polyketide synthases in the herb Curcuma longa", J Biol Chem, 284, pp.11160~11170 (2009)
- Lan TTP, "Identification and characterization of genes in the curcuminoid pathway of Curcuma zedoaria Roscoe", Curr Pharm Biotechnol, 19, pp.839~846 (2018)
- Lee J, "Secondary metabolite profiling of Curcuma species grown at different locations using GC", TOF and UPLC, Molecules, pp.2014, 19[DOI]
- Lenka SK, "Identification and expression analysis of methyl jasmonate responsive ESTs in paclitaxel producing Taxus cuspidate suspension culture cells", BMC Genomics, 13, pp.148~ (2012)
- Lobo R, "Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties", J Pharm Pharmacol, 61, pp.13~21 (2009)
- Loc NH, "Effect of salicylic acid on expression level of genes related with isoprenoid pathway in centella (Centella asiatica (L.) Urban) cells", 3 Biotech, 6, pp.86~ (2016)
- Loc NH, "Accumulation of madecassoside - a major component of centelloside - in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid", Period Biol, 119, pp.81~85 (2017)
- Loi DT, "Medicinal plants and Vietnamese traditional medicines" (2004), Medical Publishing House
- Murashige T, "A revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Namdeo AG, "Plant cell elicitation for production of secondary metabolites: A review", Phcog Rev, 1, pp.69~79 (2007)
- Park WT, "Yeast extract and silver nitrate induce the expression of phenylpropanoid biosynthetic genes and induce the accumulation of rosmarinic acid in Agastache rugosa cell culture", Molecules, 21, pp.426~ (2016)
- Qiao F, "The cytoskeleton enhances gene expression in the response to the Harpin elicitor in grapevine", J Exp Bot, 61, pp.4021~4031 (2010)
- Rahimi S, "Effect of salicylic acid and yeast extract on the accumulation of jasmonic acid and sesquiterpenoids in Panax ginseng adventitious roots", Russ J Plant Physiol, 61, pp.811~817 (2014)
- Sandeep IS, "Differential expression of CURS gene during various growth stages, climatic condition and soil nutrients in turmeric (Curcuma longa): Towards site specific cultivation for high curcumin yield", Plant Physiol Biochem, 118, pp.348~355 (2017)
- Wang B, "Effect of salicylic acid on cell growth and polysaccharide production in suspension cultures of protocorm-like bodies from Dendrobium huoshanense", Chinese J Biotechnol, 25, pp.1062~1068 (2009)
- Yamamoto H, "Stimulation of prenylated flavanone production by mannans and acidic polysaccharides in callus culture of Sophora flavescens", Phytochem, 40, pp.77~81 (1995)
- Zhao JL, "Effects of biotic and abiotic elicitors on cell growth and tanshinone accumulation in Salvia miltiorrhiza cell cultures", Appl Microbiol Biotechnol, 87, pp.137~144 (2010)
