Enhancing Production of Terpenoids in Metabolically Engineered Transgenic Spearmint (Mentha spicata L.) by Salt and Fungal Elicitors
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Forest tree species usually takes for long periods to be harvested and cultivated but spearmints are a good model system for woody plant because of reducing and shortening cultivation time. Spearmints are good model plants (Mentha species) for research about terpenoids production and industrial essential oil manufacture. Isopentenyl pyrophosphate isomerase (Iso) and limonene synthase (Limo) are the key enzymes of terpenoid biosynthesis pathway. Transgenic and wild spearmints (Mentha spicata, MS) were cultured in vitro and assessed for the essential oil contents. The content of essential oil of transgenic spearmint also was enhanced slightly depending on the target terpenoid genes. In an attempt to increase productivity of terpenoids further, salt and fungal elicitation strategy was adopted on transgenic Mentha spicata. The salt (800 mM NaCl) as abiotic and two fungi (Botrytis cinerea and Glomerella cingulata) as biotic were used for elicitors. In the absence of salt stress four terpenoids were detected from the spearmint extracts, all of them being monoterpenes. On the other hand, the transgenic (MSIso) extracts contained eleven terpenoids (10 monoterpenes and 1 phenylpropene) while transgenic (MSLimo) extracts contained seven monoterpenes. After 3 days of fungal infection, the resistance indices further increased to 4.38, 3.89 and 2.04 for wild type, MSIso and MSLimo, respectively. The salt and fungal elicitators proved beneficial towards modifying both the terpenoids profile and improvement in the composition of essential oil. These results have important applications for the large-scale production of essential oils and forest biotechnology with respect to spearmint.
Abstract
Forest tree species usually takes for long periods to be harvested and cultivated but spearmints are a good model system for woody plant because of reducing and shortening cultivation time. Spearmints are good model plants (Mentha species) for research about terpenoids production and industrial essential oil manufacture. Isopentenyl pyrophosphate isomerase (Iso) and limonene synthase (Limo) are the key enzymes of terpenoid biosynthesis pathway. Transgenic and wild spearmints (Mentha spicata, MS) were cultured in vitro and assessed for the essential oil contents. The content of essential oil of transgenic spearmint also was enhanced slightly depending on the target terpenoid genes. In an attempt to increase productivity of terpenoids further, salt and fungal elicitation strategy was adopted on transgenic Mentha spicata. The salt (800 mM NaCl) as abiotic and two fungi (Botrytis cinerea and Glomerella cingulata) as biotic were used for elicitors. In the absence of salt stress four terpenoids were detected from the spearmint extracts, all of them being monoterpenes. On the other hand, the transgenic (MSIso) extracts contained eleven terpenoids (10 monoterpenes and 1 phenylpropene) while transgenic (MSLimo) extracts contained seven monoterpenes. After 3 days of fungal infection, the resistance indices further increased to 4.38, 3.89 and 2.04 for wild type, MSIso and MSLimo, respectively. The salt and fungal elicitators proved beneficial towards modifying both the terpenoids profile and improvement in the composition of essential oil. These results have important applications for the large-scale production of essential oils and forest biotechnology with respect to spearmint.
참고문헌 (45)
- Abraham WR, "Microbial hydroxylation of cedrol and cedrene", Z Naturforsch, 42C, pp.414~419 (1987)
- Aoki T, "Suddendeath syndrome of soybean is caused by two morphologically and phylogenetically distinct species within the Fusarium solani species complex--F. virguliforme in North America and F. tucumaniae in South America", Mycologia, 95, pp.660~684 (2003)
- Aoki T, "Sudden death syndrome of soybean in South America is caused by four species of Fusarium : Fusarium brasiliense sp. nov., F. cuneirostrum sp. nov., F. tucumaniae, and F. virguliforme", Mycoscience, 46, pp.162~183 (2005)
- Native and Scotch Spearmint Oil Production in Tasmania and Victoria, "Bienvenu F", A report for the Rural Industries Research and Development Corporation, 99~147 (1999)
- Bryant JP, "Ontogenic development of chemical defense by seedling resin birch : Energy cost of defense production", J Chem Ecol, 21, pp.883~896 (1995)
- Bryant JP, "Interactions between woody plants and browsing mammals mediated by secondary metabolites", Annu Rev Ecol Syst, 22, pp.431~446 (1991)
- Budavari S, "The Merck index: an encyclopedia of chemicals, drugs, and biologicals" (1996), Merck & Co Inc
- Burdock GA, "Fenaroli’s Hanbook of Flavor In-gredients:Adapted from the Italian Language Works of Giovanni Fenaroli" (1995), CRC Press
- Burt SA, "Increase in activity of essential oil components carvacrol and thymol against Escherichia coli O157 : H7 by addition of food stabilizers", J Food Prot, 68, pp.919~926 (2005)
- 최민선, "Expression of hpa1 Gene Encoding a Bacterial Harpin Protein in Xanthomonas oryzae pv. oryzae Enhances Disease Resistance to Both Fungal and Bacterial Pathogens in Rice and Arabidopsis", The Plant Pathology Journal, 28(4), pp.364~372 (2012), 한국식물병리학회
- Corazza M, "Allergic contact cheilitis due to carvone in toothpastes", Contact Dermatitis, 46, pp.366~367 (2002)
- Davidson PM, "Phyto-phenols. In: Natural Food Antimicrobial Systems", pp.265~293 (2000), CRC Press
- Dorman HJ, "Antimicrobial agents from plants: antibacterial activity of plant volatile oils", J Appl Microbiol, 88, pp.308~316 (2000)
- Erman WF, "Chemistry of the monoterpenes: an encyclopedic handbook" (1985), Marcel Dekker
- Ghelardini C, "Local anaesthetic activity of beta-caryophyllene", Farmaco, 56, pp.387~389 (2001)
- Gouinguené SP, "The effects of abiotic factors on induced volatile emissions in corn plants", Plant Physiol, 129, pp.1296~1307 (2002)
- Hall RL, "Recent progress in the consideration of flavoring ingredients under the food additives amendment", III GRAS Substances. Food Technol, 19, pp.151~197 (1965)
- Himejima M, "Antimicrobial terpenes from oleoresin of ponderosa pine tree Pinus ponderosa: A defense mechanism against microbial invasion", J Chem Ecol, 18, pp.1809~1818 (1992)
- Julkunen-Tiitto R, "Slight tissue wounding fails to induce consistent chemical defense in three willow(Salix spp. )clones", Oecologia, 101, pp.467~471 (1995)
- Jung HY, "Enhanced production of scopolamine by bacterial elicitors in adventitious hairy root cultures of Scopolia parviflora", Enzyme Microb Tech, 33, pp.987~990 (2003)
- 강영민, "Comparative Analysis of Terpenoids in in vitro Culture Media of Metabolically Engineered Transgenic and Wild Type Spearmint (Mentha spicata L.)", 한국약용작물학회지, 20(5), pp.301~307 (2012), 한국약용작물학회
- Kieslich K, "Transformations of terpenoids" (1986), Walter de Gruyter and Co
- Laitinen ML, "Foliar phenolic composition of European white birch during bud unfolding and leaf development", Physiol Plant, 114, pp.450~460 (2002)
- Laitinen ML, "Variation in birch bark secondary chemistry between and within clones : implications for herbivory by hares", Oikos, 104, pp.316~326 (2004)
- Lamare V, "Microbial transformations. IX : Biohydroxylation of alpha-cedrene and cedrol. Synthesis of an odoriferous minor component of cedar wood essential oil", Tetrahedron Lett, 28, pp.6269~6272 (1987)
- Lamare V, "Bioconversion of sesquiterpenes", Tetrahedron, 46, pp.4109~4132 (1990)
- Lange BM, "Improving peppermint essential oil yield and composition by metabolic engineering", Proc Natl Acad Sci USA, 108, pp.16944~16949 (2011)
- Lawrence RV, "Processing pine gum into turpentine and rosin" (1989), Pulp Chemicals Association
- 이대원, "Identification and Characterization of Expansins from Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae)", The Plant Pathology Journal, 28(4), pp.409~417 (2012), 한국식물병리학회
- Li YH, "Determination of Vanillin, Eugenol and Isoeugenol by RP-HPLC", Chromatographia, 60, pp.709~713 (2004)
- Tasmannia lanceolata: developing a new commercial flavour product: a report for the Rural Industries Research and Development Corporation, "Menary RC", ~ (1999)
- Mitsuhara I, "Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants", Plant Cell Physiol, 37, pp.49~59 (1996)
- Nickerson GB, "Gas chromatographic evidence for the occurrence of hop oil components in beer", J Chromatogr, 21, pp.1~5 (1996)
- Park DJ, "Metabolites profiling and its function through terpenoids biosynthesis genes overexpression of spearmint (MenthaspicataL.)", 석사, pp.2008
- Sambrook J, "A laboratory manual" (1998), Cold Spring Harbor Lab
- Seigler DS, "Plant Secondary Metabolism" (1998), Kluwer Academic Publishers
- Singh HP, "Autotoxicity: concept, organisms, and ecological significance", CR Rev Plant Sci, 18, pp.757~772 (1999)
- Sköld M, "The fragrance chemical beta-caryophyllene-air oxidation and skin sensitization", Food Chem Toxicol, 44, pp.538~545 (2006)
- Smith BPC, "β-caryophyllene in the skin secretion of the Australian green tree frog, Litoria caerulea: an investigation of dietary sources", Aust J Zool, 52, pp.521~530 (2004)
- Tambe Y, "Gastric cytoprotection of the non-steroidal anti-inflammatory sesquiterpene, beta-caryophyllene", Planta Med, 62, pp.469~470 (1996)
- Tegelberg R, "Quantitative changes in secondary metabolites of dark-leaved willow (Salix myrsinifolia) exposed to enhanced ultraviolet-B radiation", Physiologia Plantarum, 113, pp.541~547 (2001)
- Tegelberg R, "The effects of long-term elevated UV-B on the growth and phenolics of field-grown silver birch (Betula pendula)", Global Change Biology, 7, pp.839~848 (2001)
- Ultee A, "The phenolic hydroxyl group of carvacrol is essential for action against the food-borne pathogen Bacillus cereus", Appl Environ Microbiol, 68, pp.1561~1568 (2002)
- Ultee A, "Antimicrobial activity of carvacrol toward Bacillus cereus on rice", J Food Prot, 63, pp.620~624 (2000)
- Waterman PG, "Analysis of phenolic plant metabolites" (1994), Blackwell Scientific Publications
