Improvement of cadmium tolerance and accumulation of Phragmites spp. Tabarka by ethyl methane sulfonate mutagenesis
0
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M2 mutant reeds under Cd stress conditions (up to 1,500 μM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.
Abstract
Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M2 mutant reeds under Cd stress conditions (up to 1,500 μM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.
참고문헌 (24)
- Ali H, "Environmental chemistry and ecotoxicology of hazardous heavy metals: environmental persistence, toxicity, and bioaccumulation", J Chem, 2019 (2019)
- Ali NA, "Tolerance and bioaccumulation of cadmium by Phragmites australis grown in the presence of elevated concentrations of cadmium, copper, and zinc", Aquat Bot, 80, pp.163~176 (2004)
- Arthur EL, "Phytoremediation-an overview", Crit Rev Plant Sci, 24, pp.109~122 (2005)
- Benavides MP, "Cadmium toxicity in plants", Braz J Plant Physiol, 17(1), pp.21~34 (2005)
- Chen J, "Phytoremediation of soil and water contaminants", pp.264~ (1997), American Chemical Society
- Cherian S, "Transgenic Plants in Phytoremediation: Recent Advance ad New Possibilities", Environ Sci Technol, 39(24), pp.9377~9390 (2005)
- Cresser MS, "Sulfuric-perchloric acid digestion of plant material for the determination of nitrogen, phosphorus, potassium, calcium and magnesium", Anal Chem Acta, 109, pp.431~436 (1979)
- Das N, "Enhanced cadmium accumulation and tolerance in transgenic tobacco overexpressing rice metal tolerance protein gene OsMTP1 is promising for phytoremediation", Plant Physiol Biochem, 105, pp.297~309 (2016)
- Das P, "Studies on cadmium toxicity in plants: a review", Environ Pollut, 98, pp.29~36 (1997)
- Ederli L, "Response induced by high concentration of cadmium in Phragmites australis roots", Physiol Plant, 121, pp.66~74 (2004)
- Gunarathne V, "Transgenic Plant Technology for Remediation of Toxic Metals and Metalloids", pp.89~102 (2019), Elsevier
- Hill MK, "Understanding environmental pollution" (2010), Cambridge University Press
- Higuchi K, "Common reed accumulates starch in its stem by metabolic adaptation under Cd stress conditions", Front Plant Sci, 6, pp.138~ (2015)
- 김계훈, "Expression of Catalase (CAT) and Ascorbate Peroxidase (APX) in MuSI Transgenic Tobacco under Cadmium Stress", 한국토양비료학회지(Korean Journal of Soil Science and Fertilizer), 44(1), pp.53~57 (2011), 한국토양비료학회
- Young-Nam Kim, "Cadmium resistance in tobacco plants expressing the MuSI gene", Plant Biotechnology Reports, 5(4), pp.323~329 (2011), 한국식물생명공학회
- Maple J, "Circadian Rhythms", pp.197~206 (2007), Humana Press
- Mantovi P, "Application of a horizontal subsurface flow constructed wetland on treatment of dairy parlor wastewater", Bioresource Technol, 88, pp.85~94 (2003)
- Negri MC, "Plants that remove contaminants from the environment", Lab Med, 27, pp.36~39 (1996)
- Nehnevajova E, "Metal accumulation and response of antioxidant enzymes in seedlings and adult sunflower mutants with improved metal removal traits on a metal-contaminated soil", Environ Exp Botany, 76, pp.39~48 (2012)
- Phang IC, "Chemical mutagenesis for improving potential of plants to remediate environments with heavy metal contaminants", Curr Top Med Chem, 11, pp.47~52 (2012)
- Poonawala IS, "Factors influencing bud break and mass-scale micropropagation of three Phragmites species: P. karka, P. communis, and P. australis", Plant Cell Rep, 18, pp.696~700 (1999)
- Stoltz E, "Accumulation properties of As, Cd, Cu, Pb and Zn by four wetland plant species growing on submerged mine tailings", Environ Exp Bot, 47, pp.271~280 (2002)
- Toppi LS, "Response to cadmium in higher plants", Environ Exp Bot, 41(2), pp.105~130 (1999)
- Ullah S, "Comparative study of growth, cadmium accumulation and tolerance of three chickpea (Cicer arietinum L.) cultivars", Plants, 9(3), pp.310~ (2020)