Functional characterization of gibberellin signaling-related genes in Panax ginseng
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초록
Gibberellins (GAs) are essential phytohormones for plant growth that influence developmental processes and crop yields. Recent functional genomic analyses of model plants have yielded good characterizations of the canonical GA signaling pathways and related genes. Although Panax ginseng has long been considered to have economic and medicinal importance, functional genomic studies of the GA signaling pathways in this crucial perennial herb plant have been rarely conducted. Here, we identified and performed functional analysis of the GA signaling-related genes, including PgGID1s, PgSLY1s, and PgRGAs. We confirmed that the physiological role of GA signaling components in P. ginseng was evolutionarily conserved. In addition, the important functional domains and amino acid residues for protein interactions among active GA, GID1, SCFSLY1, and RGA were also functionally conserved. Prediction and comparison of crystallographic structural similarities between PgGID1s and AtGID1a supported their function as GA receptors. Moreover, the subcellular localization and GAdependent promotion of DELLA degradation in P. ginseng was similar to the canonical GA signaling pathways in other plants. Finally, we found that overexpression of PgRGA2 and PgSLY1-1 was sufficient to complement the GA-related phenotypes of atgid1a/c double- and rga quintuple-mutants, respectively. This critical information for these GA signaling genes has the potential to facilitate future genetic engineering and breeding of P. ginseng for increased crop yield and production of useful substances.
Abstract
Gibberellins (GAs) are essential phytohormones for plant growth that influence developmental processes and crop yields. Recent functional genomic analyses of model plants have yielded good characterizations of the canonical GA signaling pathways and related genes. Although Panax ginseng has long been considered to have economic and medicinal importance, functional genomic studies of the GA signaling pathways in this crucial perennial herb plant have been rarely conducted. Here, we identified and performed functional analysis of the GA signaling-related genes, including PgGID1s, PgSLY1s, and PgRGAs. We confirmed that the physiological role of GA signaling components in P. ginseng was evolutionarily conserved. In addition, the important functional domains and amino acid residues for protein interactions among active GA, GID1, SCFSLY1, and RGA were also functionally conserved. Prediction and comparison of crystallographic structural similarities between PgGID1s and AtGID1a supported their function as GA receptors. Moreover, the subcellular localization and GAdependent promotion of DELLA degradation in P. ginseng was similar to the canonical GA signaling pathways in other plants. Finally, we found that overexpression of PgRGA2 and PgSLY1-1 was sufficient to complement the GA-related phenotypes of atgid1a/c double- and rga quintuple-mutants, respectively. This critical information for these GA signaling genes has the potential to facilitate future genetic engineering and breeding of P. ginseng for increased crop yield and production of useful substances.
참고문헌 (34)
- Achard P, "Releasing the brakes of plant growth : how GAs shutdown DELLA proteins", Journal of experimental botany, 60(4), pp.1085~1092 (2009)
- Ariizumi T, "The role of two F-box proteins, SLEEPY1 and SNEEZY, in Arabidopsis gibberellin signaling", Plant physiology, 155(2), pp.765~775 (2011)
- Bari R, "Role of plant hormones in plant defence responses", Plant molecular biology, 69(4), pp.473~488 (2009)
- Cheng H, "Gibberellin regulates Arabidopsis floral development via suppression of DELLA protein function", Development, 131(5), pp.1055~1064 (2004)
- Dill A, "The DELLA motif is essential for gibberellin-induced degradation of RGA", Proceedings of the National Academy of Sciences, 98(24), pp.14162~14167 (2001)
- Dill A, "Synergistic derepression of gibberellin signaling by removing RGA and GAI function in Arabidopsis thaliana", Genetics, 159(2), pp.777~785 (2001)
- Dill A, "The Arabidopsis F-box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation", The Plant Cell, 16(6), pp.1392~1405 (2004)
- Fu X, "Gibberellin-mediated proteasome-dependent degradation of the barley DELLA protein SLN1 repressor", The Plant Cell, 14(12), pp.3191~3200 (2002)
- Griffiths J, "Genetic characterization and functional analysis of the GID1 gibberellin receptors in Arabidopsis", The Plant Cell, 18(12), pp.3399~3414 (2006)
- Harberd NP, "The angiosperm gibberellin-GID1-DELLA growth regulatory mechanism : how an"inhibitor of an inhibitor"enables flexible response to fluctuating environments", The Plant Cell, 21(5), pp.1328~1339 (2009)
- Hirano K, "The GID1-mediated gibberellin perception mechanism is conserved in the lycophyte Selaginella moellendorffii but not in the bryophyte Physcomitrella patens", The Plant Cell, 19(10), pp.3058~3079 (2007)
- Hong CP, "Gibberellin signaling promotes the secondary growth of storaage roots in Panax ginseng", International Journal of Molecular Science, 22, pp.8694~ (2021)
- 홍정의, "Identification of ABSCISIC ACID (ABA) signaling related genes in Panax ginseng", Journal of Plant Biotechnology, 45(4), pp.306~314 (2018), 한국식물생명공학회
- Hu SY, "The genusPanax(ginseng)in Chinese medicine", Economic Botany, 30(1), pp.11~28 (1976)
- Jang H-J, "Anticarcinogenic effects of products of heat-processed ginsenoside Re, a major constituent of ginseng berry, on human gastric cancer cells", Journal of agricultural and food chemistry, 62(13), pp.2830~2836 (2014)
- Jo I-H, "Isoform sequencing provides a more comprehensive view of the panax ginseng transcriptome", Genes, 8(9), pp.228~ (2017)
- King KE, "Gibberellins are not required for normal stem growth in Arabidopsis thaliana in the absence of GAI and RGA", Genetics, 159(2), pp.767~776 (2001)
- Ku mar S, "M EGA7 : molecu lar evolutionary genetics analysis version 7. 0 for bigger datasets", Molecular biology and evolution, 33(7), pp.1870~1874 (2016)
- Lee J, "Brassinosteroid-BZR1", 2-WAT1module determines the high level of auxin signalling in vascular cambium during wood formation, 2021, pp.230(4), 1503~1516
- Lee S, "Gibberellin regulates Arabidopsis seed germination via RGL2, a GAI", RGA-like gene whose expression is up-regulated following imbibition, 2002, pp.16(5), 646~658
- McGinnis KM, "The Arabidopsis SLEEPY1 gene encodes a putative F-box subunit of an SCF E3 ubiquitin ligase", The Plant Cell, 15(5), pp.1120~1130 (2003)
- Nelson SK, "Gibberellin hormone signal perception: down‐regulating DELLA repressors of plant growth and development", Annual Plant Reviews online, pp.153~187 (2018)
- Olszewski N, "Gibberellin signaling:biosynthesis, catabolism, and response pathways", The Plant Cell, 14(suppl 1), pp.S61~S80 (2002)
- Reid JB, "Plant hormone mutants", Journal of Plant Growth Regulation, 12(4), pp.207~226 (1993)
- Ross JJ, "Gibberellin mutants", Physiologia Plantarum, 100(3), pp.550~560 (1997)
- Sasaki A, "Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant", Science, 299(5614), pp.1896~1898 (2003)
- Seo E, "Ginseng berry extract supplementation improves age-related decline of insulin signaling in mice", Nutrients, 7(4), pp.3038~3053 (2015)
- Sun T-p, "Gibberellin-GID1-DELLA : a pivotal regulatory module for plant growth and development", Plant physiology, 154(2), pp.567~570 (2010)
- Sun T-p, "The molecular mechanism and evolution of the GA –GID1–DELLA signaling module in plants", Current Biology, 21(9), pp.R338~R345 (2011)
- Tyler L, "DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis", Plant physiology, 135(2), pp.1008~1019 (2004)
- Ueguchi-Tanaka M, "GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin", Nature, 437(7059), pp.693~ (2005)
- Nomar Espinosa Waminal, "Characterization of Chromosome-Specific Microsatellite Repeats and Telomere Repeats Based on Low-Coverage Whole-Genome Sequence Reads in Panax ginseng", Plant Breeding and Biotechnology, 6(1), pp.74~81 (2018), 한국육종학회[DOI]
- Xu J, "Panax ginseng genome examination for ginsenoside biosynthesis", Gigascience, 6(11) (2017)
- Yasukawa K, "Inhibitory effects of ginseng(Panax ginseng)berry on tumour promotion and inflammatory ear oedema induced by TPA", Journal of Nutritional Therapeutics, 4(4), pp.143~148 (2016)