개갑된 인삼종자 휴면 조기타파에 미치는 GA3 및 변온처리 효과
Effects of Gibberellic Acid and Alternating Temperature on Breaking Seed Dormancy of Panax ginseng C. A. Meyer
2
조회
0
다운로드
13
인용
0
참고문헌
키워드
초록
Background: Developing new ginseng cultivars is a significant time-consuming process owing to the three years of growth required for ginseng to flower. To shorten the ginseng breeding process, it is necessary to establish rapid progression through each generation. In this study, we examined it was possible to rapidly break ginseng seed dormancy using gibberellic acid (GA3) treatment and alternating temperature. Methods and Results: Seeds were obtained from local variety. Seeds were treated with either GA3 at a concentration of 100 ㎎/ℓ, constant temperature (−2℃ and 2℃), alternating temperature (2℃ followed by −2℃, followed by 2℃) or a combination GA3 and temperature treatment. Following experimental treatment, seeds were sown into trays and placed in a greenhouse. Low germination rates were observed in seeds that did not receive GA3 treatment, which were similar following 2℃ and −2℃ constant temperature treatment. Germination rates increased in proportion to GA3 and more so when combined with alternating temperature treatment. In additon, stem and leaf lengths of the resulting ginseng plants were increased following GA3 treatment, although no synergistic effect was observed with alternating temperature treatment. Conclusions: These results suggest that a combination GA3 and alternating temperature treatment enhances ginseng seed germination, which can contribute to shortening the time required to progress through a single ginseng generation for breeding.
Abstract
Background: Developing new ginseng cultivars is a significant time-consuming process owing to the three years of growth required for ginseng to flower. To shorten the ginseng breeding process, it is necessary to establish rapid progression through each generation. In this study, we examined it was possible to rapidly break ginseng seed dormancy using gibberellic acid (GA3) treatment and alternating temperature. Methods and Results: Seeds were obtained from local variety. Seeds were treated with either GA3 at a concentration of 100 ㎎/ℓ, constant temperature (−2℃ and 2℃), alternating temperature (2℃ followed by −2℃, followed by 2℃) or a combination GA3 and temperature treatment. Following experimental treatment, seeds were sown into trays and placed in a greenhouse. Low germination rates were observed in seeds that did not receive GA3 treatment, which were similar following 2℃ and −2℃ constant temperature treatment. Germination rates increased in proportion to GA3 and more so when combined with alternating temperature treatment. In additon, stem and leaf lengths of the resulting ginseng plants were increased following GA3 treatment, although no synergistic effect was observed with alternating temperature treatment. Conclusions: These results suggest that a combination GA3 and alternating temperature treatment enhances ginseng seed germination, which can contribute to shortening the time required to progress through a single ginseng generation for breeding.
참고문헌 (29)
- Alpi A, "Growth regulator levels in embryo and suspensor of Phaseolus coccineus at two stages of development", Planta, 127, pp.153~162 (1975)
- Balaguera-López HE, "Effect of gibberellic acid(GA3)on seed germination and growth of tomato(Solanum lycopersicum L. )", Acta Horticulture, 821, pp.141~148 (2009)
- Bello IA, "Effects of stratification, temperature, and oxygen on woolly cupgrass(Eriochloa villosa)seed dormancy", Weed Science, 46, pp.526~529 (1998)
- Choi SY, "Effect of plant growth regulators on the germination and seedling growth of Wasabia japonica Matsum seeds", Korean Journal of Medicinal Crop Science, 3, pp.111~115 (1995)
- Duclos DV, "Investigating seed dormancy in switchgrass(Panicum virgatum L. ) : Elucidating the effect of temperature regimes and plant hormones on embryo dormancy", Industrial Crops and Products, 58, pp.148~159 (2014)
- Finch-Savage WE, "Seed dormancy and the control of germination", New Phytologist, 171, pp.501~523 (2006)
- Groot SP, "Gibberellins regulate seed germination in tomato by endosperm weakening: A study with gibberellin-deficient mutants", Planta, 171, pp.525~531 (1987)
- 최인수, "식물생장조절제 및 priming 처리가 금어초 종자의 발아와 초기생육에 미치는 영향", 생명과학회지, 16(3), pp.493~499 (2006), 한국생명과학회
- Karssen CM, "Key role for endogenous gibberellins in the control of seed germination", Annals of Botany, 63, pp.71~80 (1989)
- 김동휘, "인삼 묘삼 휴면 조기타파에 미치는 GA3 및 변온 처리 효과", 한국약용작물학회지, 23(5), pp.379~384 (2015), 한국약용작물학회[DOI]
- 김명석, "황금 종자 안정생산을 위한 발아특성, 파종적기, 비가림 시설효과", 한국약용작물학회지, 24(2), pp.101~109 (2016), 한국약용작물학회[DOI]
- 김영창, "인삼 종자의 개갑률 향상을 위한 적정 수확시기 및 GA3 처리 효과", 한국약용작물학회지, 22(6), pp.423~428 (2014), 한국약용작물학회[DOI]
- 김윤하, "식물생장조절물질 처리가 서양민들레 종자 발아에 미치는 영향", Weed & Turfgrass Science, 2(2), pp.152~158 (2013), 한국잔디학회
- Kwon WS, "Identification and changes of physiologically active substances during chilling storage of dehisced ginseng seeds", Journal of Ginseng Research, 21, pp.13~18 (1997)
- Kwon WS, "Effects of growth regulators on the germination of Panax ginseng C. A. Meyer", Journal of Ginseng Research, 10, pp.159~166 (1986)
- Kwon WS, "Optimum chilling terms for germination of the dehisced ginseng(Panax ginseng C. A. Meyer)seed", Journal of Ginseng Research, 25, pp.167~170 (2001)
- Kwon WS, "Changes of cytokinins and gebberellin contents during low temperature storage of dehisced ginseng seeds", Journal of Korea Society Horticulture Science and Biotechnology, 38, pp.111~115 (1997)
- Lee JC, "Dormancy of ginseng seed as influenced by temperature and gibberellic acids", Korean Journal of Ginseng Science, 31, pp.220~225 (1986)
- 이수광, "종자저장온도 및 전처리가 야생 하늘타리와 노랑하늘타리의 종자발아 및 유묘 생육특성에 미치는 영향", 한국약용작물학회지, 22(1), pp.53~59 (2014), 한국약용작물학회[DOI]
- Lippai A, "Effects of temperature and water potential on germination of horehound(Marrubium vulgare)seeds from two Australian localities", Weed Science, 44, pp.91~99 (1996)
- 오욱, "Light Intensity and Temperature Regulate Petiole Elongation by Controlling the Content of and Sensitivity to Gibberellin in Cyclamen persicum", Horticulture, Environment, and Biotechnology, 55(3), pp.175~182 (2014), 한국원예학회[DOI]
- Park H, "Effect of gibberellin and kinetin on bud dormancy breaking and growth of Korean ginseng root(Panax ginseng C. A. Meyer)", Korean Journal of Ginseng Science, 3, pp.105~112 (1979)
- Picciarelli P, "Gibberellin-like activity in suspensors of Tropaeolum majus L. and Cytisus laburnum L", Planta, 162, pp.566~568 (1984)
- Ross JD, "Studies in seed dormancy. V. The content of endogenous gibberellins in seeds of Corylus avellana L", Planta, 100, pp.288~302 (1971)
- Scott SJ, "Review of data analysis methods for seed gemination", Crop Science, 24, pp.1192~1199 (1984)
- Son ER, "Preliminary studies on breaking of dormancy and germination of Panax ginseng seeds", Korean Journal of Crop Science, 22, pp.45~51 (1977)
- Sundstrom FJ, "Effect of seed treatment and planting method on tabasco pepper", Journal of the American Society for Horticultural Science, 112, pp.641~644 (1987)
- Toh S, "High temperature-induced abscisic acid biosynthesis and its role in the inhibition of gibberellin action in Arabidopsis seeds", Plant Physiology, 146, pp.1368~1385 (2008)
- Tzortzakis NG, "Effect of pre-sowing treatment on seed germination and seedling vigour in endive and chicory", Horticultural Science(Prague), 36, pp.117~125 (2009)