Alocasia amazonica의 생물반응기 배양에서 배지 공급 방식이 식물체의 생장과 잎조직 및 기공의 특성에 미치는 영향
Plantlet Growth, and Leaf and Stomatal Characteristics of Alocasia amazonicaas Affected by Medium Supply Methods in Bioreactor Culture
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Abstract
Comparative studies on medium supply in bioreactors (raft, immersion and ebb and flood) have revealed that multiplication and growth of Alocasia Amazonica were greatest in the raft system, while lowest in ebb and flood system. In the raft system, the basal part of the shoots was continuously in contact with medium, which enabled a constant uptake of nutrients as well as aeration to the explants. The number and the size of leaf stomata were higher in the raft system compared with immersion and ebb&flood system. In the immersion system, plantlets were deformed and epidermal cells in leaves were irregular with a large intercellular space. The results suggested that the medium supply should be controlled properly to maintain normal and healthy plantlets during liquid cultures in bioreactors which affects morphology and physiology of the plantlets.