들깨 γ-TMT 형질전환 담배의 색소성분 변화 및 염 스트레스 내성 증가
Transgenic tobacco with γ-TMT of perilla showed increased salt resistance and altered pigment synthesis
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Tocopherols are essential lipophilic antioxidant in human cells, while little is known about its function in plant tissues. To study the impact of composition and content of tocopherols on stress tolerance, tobacco (Nicotiana tabacum) was transformed with a construct containing a cDNA insert encoding γ-tocopherol methyltransferase (γ-TMT/VTE4) from perilla under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco was confirmed by PCR and RT-PCR. The total content and composition of tocopherols in the transgenic lines were similar with wild type controls. However, chlorophyll-a and carotenoid content in the transgenic lines were increased by up to 45% (P< 0.01) and 39% (P< 0.02), respectively. Also, the over-expression of γ-TMT increased the salt stress tolerance in tobacco plants. These results demonstrate that over-expression of γ-TMT gene in tocopherol bio-synthetic pathway can increase salt stress tolerance and contents of chlorophyll-a and carotenoid in transgenic tobacco plants.
Abstract
Tocopherols are essential lipophilic antioxidant in human cells, while little is known about its function in plant tissues. To study the impact of composition and content of tocopherols on stress tolerance, tobacco (Nicotiana tabacum) was transformed with a construct containing a cDNA insert encoding γ-tocopherol methyltransferase (γ-TMT/VTE4) from perilla under the control of the cauliflower mosaic virus (CaMV) 35S promoter. The transgenic tobacco was confirmed by PCR and RT-PCR. The total content and composition of tocopherols in the transgenic lines were similar with wild type controls. However, chlorophyll-a and carotenoid content in the transgenic lines were increased by up to 45% (P< 0.01) and 39% (P< 0.02), respectively. Also, the over-expression of γ-TMT increased the salt stress tolerance in tobacco plants. These results demonstrate that over-expression of γ-TMT gene in tocopherol bio-synthetic pathway can increase salt stress tolerance and contents of chlorophyll-a and carotenoid in transgenic tobacco plants.
참고문헌 (22)
- Abbasi A. R., "Specific roles of α- and γ-tocopherol in abiotic stress responses of transgenic tobacco", Plant Physiol, 143, pp.1720~1738 (2007)
- Alscher R. G. E. N., "Role of superoxide dismutases (SODs) in controlling oxidative stress in plants", J Exp Bot, 53, pp.1331~1341 (2002)
- Bergmuller E., "Characterization of an Arabidopsis mutant deficient in gammatocopherol methyltransferase", Plant Mol Biol, 52, pp.1181~1190 (2003)
- Dat J. V. S., "Dual action of the active oxygen species during plant stress responses", Cell Mol Life Sci, 57, pp.779~795 (2000)
- DellaPenna D., "Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol", Plantarum, 126, pp.356~368 (2006)
- DellaPenna D., "Vitamin synthesis in plants: tocopherols and carotenoids", Annu. Rev. Plant Biol, 57, pp.711~738 (2006)
- Fryer M. J., "The antioxidant effects of thylakoid vitamin E (α-tocopherol)", Plant Cell Environ, 15, pp.381~392 (1992)
- Fukuzawa K., "Antioxidant activities of tocopherols on Fe²+-ascorbate induced lipid peroxidation in lecithin liposomes", Lipids, 17, pp.511~513 (1982)
- Grusak M. A., "Improving the nutrient composition of plants to enhance human nutrition and health", Annu Rev Plant Physiol Plant Mol Biol, 50, pp.133~161 (1999)
- Hirschberg J., "Production of high-value compounds: carotenoids and vitamin E", Curr. Opin. Biotech, 10, pp.186~191 (1999)
- Jones A. S., "Isolation and analysis of the deoxyribonucleic acid of mycoplasma mycoides var. capri", Nature, 198, pp.588~589 (1963)
- Kamal-Eldin A., "The chemistry and antioxidant properties of tocopherols and tocotrienols", Lipids, 31, pp.671~701 (1996)
- Kim K. H., "Molecular cloning and characterization of γ -tocopherol methyltransferase cDNA from Perilla frutescens", J. Korean Soc. Agric. Chem. Biotechnol, 45, pp.203~206 (2002)
- Lichtenthaler H. K., "Chlorophylls and carotenoids: pigments of photosynthetic biomembranes", Meth Enzymol, 148, pp.350~382 (1987)
- Liu X., "Enhanced tolerance to drought stress in transgenic tobacco plants overexpressing VTE1 for increased tocopherol production from Arabidopsis thaliana", Biotechnol Lett, 30, pp.1275~1280 (2008)
- Murashige T., "revised medium for rapid growth and bioassays with tobacco tissue cultures", Physiol Plant, 15, pp.473~497 (1962)
- Panfili G., "Normal phase high-performance liquid chromatography method for the determination of tocopherols and tocotrienols in cereals", J Agric Food Chem, 51, pp.3940~3944 (2003)
- Savidge B., "Isolation and characterization of homogentisate phytyltransferase genes from Synechocystis sp. PCC 6803 and Arabidopsis", Plant Physiol, 129, pp.321~332 (2002)
- Rise M., "Accumulation of α-tocopherol in senescing organs as related to chlorophyll degradation", Plant Physiol, 89, pp.1028~1030 (1989)
- Shintani D. K., "Engineering plants for increased nutrition and antioxidant content through the manipulation of the vitamin E pathway", Genet. Eng, 27, pp.231~242 (2006)
- Soll J., "Localization and synthesis of prenylquinones in isolated outer and inner envelope membranes from spinach chloroplasts", Arch. Biochem. Biophys, 238, pp.290~299 (1985)
- Valentin H. E., "Biotechnological production and application of vitamin E: current state and prospects", Appl Microbiol Biotechnol, 68, pp.436~444 (2005)