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Paraquat 스트레스를 받는 무 (Raphanus sativus L) 유식물에서 H2O2 분해 효소에 대한 폴리아민의 효과

Effects of polyamines on hydrogen peroxide-scavenging enzymes in radish seedling plants under paraquat stress

진창덕
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2009.06|Journal of Plant Biotechnology|제36권 제2호|pp. 149-156

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초록

무 (Raphanus sativus L.) 유식물의 자엽에 polyamines (PAs) 중 특히 1 mM spermidine (Spd) 전처리 시 paraquat (PQ)에 의해 유도 되는 자엽의 산화적 손상이 경감되었다. 즉, 자엽의 생체량, 엽록 소, 단백질 함량의 소실과 자엽 내 H2O2의 과다축적과 같은 현상이 현저히 완화되었다. 이와 관련해서 H2O2 분해 효소인 catalase (CAT)와 guaiacol peroxidase (GPX)의 총활성도 변화를 조사하였으 며 그 결과, PQ에 노출된 자엽에서 PA 중 1 mM Spd 전처리에 의 하여 CAT 활성도가 크게 증가하였고 또한 PQ에 의한 급속한 GPX 활성도 감소 역시 현저히 억제되어 거의 대조구 수준을 보였다. CAT와 GPX 동위효소 조성에 대한 native gel 분석 결과, PQ에 노 출된 자엽에서 1 mM Spd 전처리 시 CAT 동위효소 1개 (CAT1)와 GPX 동위효소 1개 (GPX1)의 활성도가 현저히 증가하였으며 또한 새로운 GPX 동위효소 1개가 생성된 것을 확인하여 이 들 동위효 소가 PQ 내성 발현에 보다 큰 기여를 하고 있음을 알 수 있었다. 위 결과들로부터, PAs 중 특히 Spd가 PQ에 노출된 무 자엽에서 내 재성 H2O2 함량 수준을 조절하는 CAT와 GPX 효소의 발현 유도를 통해 항산화 보호제로서의 역할을 수행하는 것으로 생각되었다

Abstract

Application of exogenous polyamines (PAs) reduced the paraquat (PQ)-induced cotyledon injuries in radish seedling plants with 1 mM spermidine (Spd) being the most effective protectant. PQ injury symptoms in the cotyledons, e.g., large accumulation of H2O2 , and losses of fresh weight, chlorophyll, and proteins, were significantly alleviated. Likewise, analysis of H2O2-scavenging enzymes such as catalase (CAT) and guaiacol peroxidase (GPX) showed that pretreatment with Spd among PAs remarkably increased total CAT activity and strongly retarded PQ-induced rapid decline in total GPX activity. In a native gel assay, one CAT isozyme (CAT1) and two GPX isozymes (GPX1 and a newly synthesized GPX isozyme) proved to be more responsible for PQ tolerance, as manifested by the strong increases in their activities by Spd pretreatment. Based on these results, we can suggest that PAs (especially 1 mM Spd) may function as antioxidant protectors by invoking CAT and GPX enzymes which control the endogenous H2O2 level in radish cotyledons exposed to PQ.

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