낙엽송의 체세포배 발생 및 발아에 미치는 TIBA, PCIB 및 phloroglucinol의 효과
Effect of TIBA, PCIB and phloroglucinol on somatic embryo maturation and germination in Japanese larch (Larix leptolepis)
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낙엽송의 체세포배 발생 및 발아에 미치는 항옥신제 (auxin antagonist) (TIBA 및 PCIB) 혹은 옥신상승제 (auxin synergist) (phloroglucinol)의 효과를 조사하였다. 체세포배 발생 비교에는 15.8 mg/L ABA+5.0 mg/L PCIB의 첨가시 가장 많은 자엽단계의 체세포배 (177.7/90 mg ESM) 유도 가 가능하였으나 5.0 mg/L PCIB or 5.0 mg/L TIBA 첨가시 에는 체세포배가 전혀 발생되지 않았다. 이 결과는 체세 포배 발생을 위해서는 PCIB 혹은 TIBA의 단독처리만으 로는 안되고 ABA첨가 또한 필수적이라는 것을 암시한 다. 5.0 mg/L PCIB 이하의 농도 비교실험에서는 15.8 mg/L ABA+2.0 mg/L PCIB (109.3/90 mg ESM) 혹은 15.8 mg/L ABA+5.0 mg/L PCIB (103.7/90 mg ESM)의 처리구에서 가 장 높은 체세포배 유도수를 보였다. 그러나 5.0 mg/L phloroglucinol (0/90 mg ESM) 혹은 ABA 무첨가구 (3/90 mg ESM)에서는 체세포배 발생효과가 거의 없었다. 체세 포배의 발아실험에서는 가장 높은 식물체 전환율은 15.8 mg/L ABA+5.0 mg/L PCIB (67.9%)의 처리구에서 나타난 반면 5.0 mg/L PCIB 혹은 5.0 mg/L TIBA 처리구 유래 체 세포배로부터는 식물체로 전혀 전환되지 않았다.
Abstract
The effect of auxin transport inhibitor (TIBA and PCIB) or auxin synergist (phloroglucinol) on somatic embryo maturation and germination in Japanese larch (Larix leptolepis) was examined. The addition of 15.8 mg/L ABA+5.0 mg/L PCIB showed most promoted the maturation of cotyledon -staged somatic embryos (177.7/90 mg ESM). In contrast, with treatment of 5.0 mg/L PCIB or 5.0 mg/L TIBA, no somatic embryos were obtained. Considering from this result, PCIB or TIBA alone could not substitute for exogenously supplied ABA for maturation of somatic embryos. In the test of below concentration of 5.0 mg/L PCIB, the highest results were recorded in 15.8 mg/L ABA+2.0 mg/L PCIB (109.3/90 mg ESM) or 15.8 mg/L ABA+5.0 mg/L PCIB (103.7/90 mg ESM). However, 5.0 mg/L phloroglucinol (0/90 mg ESM) or no ABA addition (3/90 mg ESM) had little influence on somatic embryos maturation. In germination study, the highest frequency of plantlet regeneration obtained from the somatic embryos which had matured on 15.8 mg/L ABA+5.0 mg/L PCIB (67.9%). However, either 5.0 mg/L PCIB nor 5.0 mg/L TIBA resulted in obtained from plantlets.
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