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형질전환 효율성 향상을 위한 국화 최적 품종 선발 및 배지･배양조건설정

Selection of the fittest varieties of chrysanthemum (Dendranthemagrandiflorum Kitamura) and set of culture condition for efficienttransformation

한범수|Mi Hyang Jang|강찬호|Seung Jung Yun|So Gon Han|Sung Hwan Kown|Young Ju Song
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2009.12|Journal of Plant Biotechnology|제36권 제4호|pp. 373-383

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참고문헌

주제분류농학

초록

국화에 있어서 효율적 형질전환 체계를 구축하기 위하 여 국내외에서 육종되고 시장지배력이 강한 34개의 품종 을 수집하고 이 중 우수한 신초생성 능력을 가진 5개 품 종인 수방력, 백마, 신마, 핑크프라이드, 금수 등을 선발 하였다. 이들 품종에 적합한 생장조절제를 선발한 결과 5개 품종에 공통으로 적용할 수 있는 생장조절제 처리는 1.0 mg/L NAA와 1.0 mg/L BA의 혼용처리 이었으며 수방 력에는 3.0 mg/L NAA와 1.0 mg/L kinetin 처리가, 금수에 는 0.5 mg/L NAA와 3.0 mg/L BA 처리가 역시 효율적으로 적용되었다. 캘러스 유도와 재분화 능력을 종합적으로 검토한 결과 기본배지는 MS, 배지응고제는 phytagel 0.3% 그리고 폴리아민인 putrescine을 10-15 mg/L로 처리할 경 우 재분화율이 8% 정도 향상되었으며 배양재료로 줄기 를 사용할 경우 기관분화율이 26% 정도 향상되었으며 기관분화 소요일수도 14일 단축시킬 수 있었다

Abstract

To set efficient transformation system in chrysanthemum, thirty-four chrysanthemum (Dendranthema grandiflorum Kitamura) varieties were collected and cultured for shoot regeneration. Five varieties, ‘Shuho-no-chikara’, ‘Zinba’, ‘Baekma’, ‘Pink pride’ and ‘Keumsu’ of them were selected, because they had a high shoot regeneration efficiency. MS medium containing 1.0 mg/L NAA and BA respectively was very adequate for shoot regeneration in those varieties. MS medium with 3.0 mg/L NAA and 1.0 mg/L kinetin in ‘Shuho-no-chikara’ and the medium with 0.5 mg/L NAA and 3.0 mg/L BA in ‘Keumsu’ were also suitable for shoot regeneration. The most efficient callus induction and shoot regeneration were obtained on MS medium. Shoot regeneration was enhanced more than 8% on MS medium with 0.3% phytagel and 10-15 mg/L putrescine. The best cultural material for shoot regeneration was stem. When stem was used as a culture material, shoot regeneration rate was increased more than 26% and the days to shoot regeneration was shortened about 14 days.

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