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배 검은별무늬병(Venturia nashicola) 고도 저항성 ‘93-3-98’ 유래 PR-10 유전자의 특성

Characterization of PR-10 gene derived from highly resistant ‘93-3-98’ pear inoculated with scab (Venturia nashicola)

조강희|김세희|김대현|최인명|신일섭|천재안
한국식물생명공학회 (Korean Society for Plant Biotechnology)|2015.03|Journal of Plant Biotechnology|제42권 제1호|pp. 25-33|DOI DOI:http://dx.doi.org/10.5010/JPB.2015.42.1.25

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참고문헌

키워드

Gene expressionPyrcpPR-10Resistant degreePear Scab
주제분류농학

초록

A PyrcpPR-10 gene with differentially expressedwas isolated by using the suppression subtractive hybridizationassay between ‘93-3-98’ (highly resistant against scab causedby Venturia nashicola) and ‘Sweat Skin’(highly susceptible)and analyzed the expression pattern according to organs andcultivars. The full length of PyrcpPR-10 was cloned as743bp with 480bp’s ORP, and was determined to encode aprotein of 159 amino acid residues. On analyzing PyrcpPR-10gene sequence compared with resistant and susceptiblecultivars, ‘Hwangsilri’ (resistant), ‘Gamcheonbae’ (moderatelyresistant), ‘Wonhwang’ (moderately susceptible), ‘Niitaka’(highly susceptible), and ‘Sweat Skin’ (highly susceptible)had identical gene sequence but ‘Bartlett’ (highly resistant)showed partly different sequences. The deduced amino acidsequence showed 64 ~ 98% homology and had the GXGGXGmotif to known amino acid of other plants PR-10 by theBLAST X analysis. Among several organs or tissues, petalwas showed highest expression level of PyrcpPR-10 genefollowed by leaf, floral axis, bud, and bark. The expressionlevel of PyrcpPR-10 gene was dramatically increased at 24hr after inoculation in all cultivars and also up-regulated inaccordance with resistant degree of cultivars. While resistant cultivars (‘Bartlett’, ‘93-3-98’, and ‘Hwangsilri’) inducedrelatively high expression level of PyrcpPR-10 gene, susceptiblecultivars (‘Niitaka’, and ‘Sweat Skin’) showed lowexpression level. PyrcpPR-10 gene is assumed that it isdirectly connected with defense mechanisms to pear scab.

Abstract

A PyrcpPR-10 gene with differentially expressedwas isolated by using the suppression subtractive hybridizationassay between ‘93-3-98’ (highly resistant against scab causedby Venturia nashicola) and ‘Sweat Skin’(highly susceptible)and analyzed the expression pattern according to organs andcultivars. The full length of PyrcpPR-10 was cloned as743bp with 480bp’s ORP, and was determined to encode aprotein of 159 amino acid residues. On analyzing PyrcpPR-10gene sequence compared with resistant and susceptiblecultivars, ‘Hwangsilri’ (resistant), ‘Gamcheonbae’ (moderatelyresistant), ‘Wonhwang’ (moderately susceptible), ‘Niitaka’(highly susceptible), and ‘Sweat Skin’ (highly susceptible)had identical gene sequence but ‘Bartlett’ (highly resistant)showed partly different sequences. The deduced amino acidsequence showed 64 ~ 98% homology and had the GXGGXGmotif to known amino acid of other plants PR-10 by theBLAST X analysis. Among several organs or tissues, petalwas showed highest expression level of PyrcpPR-10 genefollowed by leaf, floral axis, bud, and bark. The expressionlevel of PyrcpPR-10 gene was dramatically increased at 24hr after inoculation in all cultivars and also up-regulated inaccordance with resistant degree of cultivars. While resistant cultivars (‘Bartlett’, ‘93-3-98’, and ‘Hwangsilri’) inducedrelatively high expression level of PyrcpPR-10 gene, susceptiblecultivars (‘Niitaka’, and ‘Sweat Skin’) showed lowexpression level. PyrcpPR-10 gene is assumed that it isdirectly connected with defense mechanisms to pear scab.

참고문헌 (35)

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