TgMTP1 과발현 애기장대에서 Nickel 흡수 연구
Studies on nickel uptake in transgenic Arabidopsis thaliana introduced with TgMTP1 gene encoding metal tolerance protein
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초록
To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both TgMTP1 (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that TgMTP1-overexpressing plants may be useful for phytoremediation of soil.
Abstract
To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both TgMTP1 (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that TgMTP1-overexpressing plants may be useful for phytoremediation of soil.
참고문헌 (13)
- Baker AJ, "Accumulators and excluders-strategies in the response of plants to heavy metals", J. Plant Nutrit, 3, pp.643~654 (1981)
- Etim EE, "Phytoremediation and Its Mechanisms", International Journal of Environment and Bioenergy, 2(3), pp.120~136 (2012)
- Gustin JL, "MTP1-dependent Zn sequestration into shoot vacuoles suggests dual roles in Zn tolerance and accumulation in Zn-hyperaccumulating plants", Plant J, 57, pp.1116~1127 (2009)
- Kil EJ, "Research of phytoremediation possibility using TgMTP1 overexpressing plants", Sungkyunkwan University Journal of Biotechnology and Bioengineering, 21, pp.1~5 (2015)
- Kim D, "The plant CDF family member TgMTP1 from the Ni", Zn hyperaccumulator Thlaspi goesingense acts to enhance efflux of Zn at the plasma membrane when expressed in Saccharomyces cerevisiae, 2004, pp.39, 237~251
- Kobae Y, "Zinc transporter of Arabidopsis thaliana AtMTP1 is localized to vacuolar membranes and implicated in zinc homeostasis", Plant Cell Physiol, 45, pp.1749~1758 (2004)
- Kramer, U, "Phytoremediation: novel approaches to cleaning up polluted soils", Curr. Opin. Biotechnol, 16, pp.133~141 (2005)
- Kupper H, "Cellular compartmentation of zinc in leaves of the hyperaccumulator Thlaspi caerulescens", Plant Physiol, 119, pp.305~311 (1999)
- Kupper H, "Cellular compartmentation of cadmium and zinc in relation to other elements in the hyperaccumulator Arabidopsis halleri", Planta, 212, pp.75~84 (2000)
- Kupper H, "Tissue- and age-dependent differences in the complexation of cadmium and zinc in the cadmium", zinc hyperaccumulator Thlaspi caerulescens (Ganges ecotype) revealed by X-ray absorption spectroscopy, 2004, pp.134, 748~757
- Persans MW, "Functional activity and role of cation-efflux family members in Ni hyperaccumulation in Thlaspi goesingense", Proc. Natl Acad. Sci. USA, 98, pp.9995~10000 (2001)
- Salt DE, "Phytoremediation", Annu. Rev.Plant physiol. Plant Mol. Biol, 49, pp.643~668 (1998)
- Weigel D, "Arabidosis A Laboratory Manual. Cold Spring Harbor" (2002), Cold Spring Harbor Laboratory Press