벼에서 Agrobacterium vector를 이용한 세포사멸 억제 유전자 도입
Transformation of Suppressor Cell Death using Agrobacterium-mediated Vector in Rice
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초록
In this study, we introduced AtBI-1 of Arabidopsis thaliana into week abiotic stress japonica rice to control cell-death expression by Agrobacterium-mediated transformation. The NPT II gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice callus. The introduction of AtBI-1 gene was approved also by PCR using AtBI-1 specific oligonucleotide primers in regenerated plantsand Southern blot analysis. Stable integration and expression of the AtBI-1-GFP gene at root, stem, and root in T0 plants and T1 progeny were confirmed by GFP analysis.
Abstract
In this study, we introduced AtBI-1 of Arabidopsis thaliana into week abiotic stress japonica rice to control cell-death expression by Agrobacterium-mediated transformation. The NPT II gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice callus. The introduction of AtBI-1 gene was approved also by PCR using AtBI-1 specific oligonucleotide primers in regenerated plantsand Southern blot analysis. Stable integration and expression of the AtBI-1-GFP gene at root, stem, and root in T0 plants and T1 progeny were confirmed by GFP analysis.
참고문헌 (20)
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