Genetic diversity analysis of Glycyrrhiza uralensis using 8 novel polymorphic microsatellite markers
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Licorice plant (Glycyrrhiza spp.) is an important herb, but the major portion of the national demand is imported to Korea because the domestic production base is vulnerable. We performed basic molecular breeding research for domestic cultivation and production. All publicly available G. uralensis EST sequences, which totaled 56,089, were assembled into 4,821 unigenes and examined for microsatellites. Eight polymorphic microsatellite loci were identified and 16 G. uralensis and 6 G. glabra accessions, which were collected from different locations, were genotyped using the microsatellites. Genetic diversity within the accessions was estimated by construction of a dendrogram. The dendrogram was clustered into two groups. The results showed that there is a correlative genetic relationship between species. The microsatellite markers were found to be useful for diversity analysis as they are able to successfully distinguish the Glycyrrhiza accessions.
Abstract
Licorice plant (Glycyrrhiza spp.) is an important herb, but the major portion of the national demand is imported to Korea because the domestic production base is vulnerable. We performed basic molecular breeding research for domestic cultivation and production. All publicly available G. uralensis EST sequences, which totaled 56,089, were assembled into 4,821 unigenes and examined for microsatellites. Eight polymorphic microsatellite loci were identified and 16 G. uralensis and 6 G. glabra accessions, which were collected from different locations, were genotyped using the microsatellites. Genetic diversity within the accessions was estimated by construction of a dendrogram. The dendrogram was clustered into two groups. The results showed that there is a correlative genetic relationship between species. The microsatellite markers were found to be useful for diversity analysis as they are able to successfully distinguish the Glycyrrhiza accessions.
참고문헌 (24)
- Asl MM, "Review of Pharmacological Effects of Glycyrrhiza sp. and its Bioactive Compounds", Phytother Res, 22, pp.709~724 (2008)
- Chevreux B, "Using the miraEST Assembler for Reliable and Automated mRNA Transcript Assembly and SNP Detection in Sequenced ESTs", Genome Res, 14, pp.1147~1159 (2004)
- Conesa A, "Blast2GO: a Universal Tool for Annotation, Visualization and Analysis in Functional Genomics Research", Bioinformatics, 21, pp.3674~3676 (2005)
- Erayman M, "Transferability of SSR Markers from Distantly Related Legumes to Glycyrrhiza species", Turk J Agric For, 38, pp.32~38 (2014)
- Kalia RK, "Microsatellite markers: An Overview If the Recent Progress in Plants", Euphytica, 177, pp.309~334 (2011)
- Khan S, "RAPD Profile for Authentication of Medicinal Plant Glycirrhiza glabra L", Medicinal Aromatic Plant Sci Biotech, 3, pp.8~51 (2009)
- Koressaar T, "Enhancements and Modifi Cations of Primer Design Program Primer3", Bioinformatics, 23, pp.1289~1291 (2007)
- Liu KJ, "PowerMarker: An Integrated Analysis Environment for Genetic Marker Analysis", Bioinformatics, 21, pp.2128~2129 (2005)
- Liu Y, "Transcriptome Analysis and Development of SSR Molecular Markers in Glycyrrhiza uralensis Fisch", PloS one, 10, pp.e0143017~ (2015)
- Morin RD, "Profiling the HeLa S3 Transcriptome using Randomly Primed cDNA and Massively Parallel Short-read Sequencing", Biotechniques, 45, pp.81~ (2008)
- Rohlf FJ, "Phylogenetic Models and Reticulations", J Classif, 17, pp.185~189 (2000)
- Rohlf FJ, "NTSYS-pc Ver. 2.11 T" (2004), Exter Software
- Roldán-Ruiz I, "AFLP Markers Reveal High Polymorphic Rates in Ryegrasses (Lolium spp.)", Mol Breeding, 6, pp.125~134 (2000)
- Sorrells ME, "Direct Classification and Selection of Superior Alleles for Crop Improvement", Crop Sci, 37, pp.691~697 (1997)
- Sudo H, "Expressed Sequence Tags from Rhizomes of Glycyrrhiza uralensis", Plant Biotechnol, 26, pp.105~107 (2009)
- Temnykh S, "Computational and Experimental Analysis of Microsatellites in Rice (Oryza sativa L.): Frequency, Length Variation, Transposon Associations, and Genetic Marker Potential", Genome Res, 11, pp.1441~1452 (2001)
- Untergasser A, "Primer3-New Capabilities and Interfaces", Nucleic Acids Res, 40, pp.e115~e115 (2012)
- Varshney RK, "Genic Microsatellite Markers: Features and Applications", Trends Biotechnol, 23, pp.48~55 (2005)
- Varshney RK, "Interspecific Transferability and Comparative Mapping of Barley EST-SSR Markers in Wheatrye and Rice", Plant Sci, 168, pp.195~202 (2005)
- Wang ML, "Microsatellite Markers in Plants and Insects. Part I: Applications of Biotechnology", Genes Genom Gen, 3, pp.54~67 (2009)
- Yamazaki M, "Genetic Relationships among Glycyrrhiza Plants Determined by RAPD and RFLP Analyses", Biol Pharm Bull, 17, pp.1529~1531 (1994)
- Yao H, "ISSR Primer Screening and Preliminary Evaluation of Genetic Diversity in Wild Populations of Glycyrrhiza uralensis", Biol Plantarum, 52, pp.117~120 (2008)
- Zhang G, "Development and Characterization of Novel EST-SSRs from Larix gmelinii and Their Cross-Species Transferability", Molecules, 20, pp.12469~12480 (2015)
- Zhang Q, "Chemical Analysis of the Chinese Herbal Medicine Gan-Cao (licorice)", J Chromatogr A, 1216, pp.1954~1569 (2009)