꿩고비(Osmunda cinnamomea var. forkiensis) 기내 포자체 재생에 영향을 미치는 배양부위와 배지구성물질
Effect of explant parts and medium components on in vitro regeneration in Osmunda cinnamomea var. forkiensis
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초록
This study was carried out to find culture materials (explant parts) and medium components (medium type, sucrose and NaH2PO4 concentration) for in vitro propagation of Osmunda cinnamomea var. forkiensis sporophyte. The results of study: chopped segments of leaf blades, stipes, rhizomes and roots were cultured on a 1/2MS medium supplemented with 0.1% activated charcoal. Among these explant types, only the rhizome segments produced young sporophyte, regenerating vigorously on a 1/8MS medium. Adjusting the sucrose concentration to 2% and supplement to 50 mg･L-1 NaH2PO4 in the 1/8MS medium proved to be more efficient for plant regeneration. Consequently, the addition of 0.1% activated charcoal to a modified 1/8MS medium (2% sucrose, 50 mg･L-1 NaH2PO4, pH 5.8 and 0.8% agar) yielded the highest sporophyte regeneration.
Abstract
This study was carried out to find culture materials (explant parts) and medium components (medium type, sucrose and NaH2PO4 concentration) for in vitro propagation of Osmunda cinnamomea var. forkiensis sporophyte. The results of study: chopped segments of leaf blades, stipes, rhizomes and roots were cultured on a 1/2MS medium supplemented with 0.1% activated charcoal. Among these explant types, only the rhizome segments produced young sporophyte, regenerating vigorously on a 1/8MS medium. Adjusting the sucrose concentration to 2% and supplement to 50 mg･L-1 NaH2PO4 in the 1/8MS medium proved to be more efficient for plant regeneration. Consequently, the addition of 0.1% activated charcoal to a modified 1/8MS medium (2% sucrose, 50 mg･L-1 NaH2PO4, pH 5.8 and 0.8% agar) yielded the highest sporophyte regeneration.
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